Presence and removal of a contaminating NADH oxidation activity in recombinant maltose-binding protein fusion proteins expressed in Escherichia coli

BioTechniques

Volumn 52, Issue 4, 2012, Pages

  Guo, Fengguang ^a   Zhu, Guan ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

Author keywords

Contamination; Fusion protein purification; His tag fusion proteins; Maltose binding protein (MBP) fusion proteins; NADH oxidation activity

Indexed keywords

AMYLOSE; DIAZEPAM BINDING INHIBITOR; FATTY ACID SYNTHASE; GLYCEROL; HYBRID PROTEIN; LIGASE; MALTOSE BINDING PROTEIN; OXIDOREDUCTASE; POLYHISTIDINE TAG; POLYKETIDE SYNTHASE; RECOMBINANT PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; RESIN; THIOL ESTER HYDROLASE; TRITON X 100; ACYL COENZYME A; CROTONYL COENZYME A; CROTONYL-COENZYME A; NICOTINAMIDE ADENINE DINUCLEOTIDE; OCTOXINOL; PROTOZOAL PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; ARTIFACT; CONTROLLED STUDY; CRYPTOSPORIDIUM PARVUM; ELUTION; ESCHERICHIA COLI; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATION; PROTEIN DOMAIN; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN PURIFICATION; ISOLATION AND PURIFICATION; METABOLISM; OXIDATION REDUCTION REACTION;

CRYPTOSPORIDIUM PARVUM; ESCHERICHIA COLI;

ACYL COENZYME A; ARTIFACTS; CRYPTOSPORIDIUM PARVUM; ESCHERICHIA COLI; GLYCEROL; MALTOSE-BINDING PROTEINS; NAD; OCTOXYNOL; OXIDATION-REDUCTION; PROTOZOAN PROTEINS; RECOMBINANT FUSION PROTEINS;

EID: 84859450213     PISSN: 07366205     EISSN: 19409818     Source Type: Journal    
DOI: 10.2144/0000113822     Document Type: Article

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