Molecular analysis of a Type I fatty acid synthase in Cryptosporidium parvum

Molecular and Biochemical Parasitology

Volumn 105, Issue 2, 2000, Pages 253-260

  Zhu, Guan ^a   Marchewka, Mary J ^a   Woods, Keith M ^b   Upton, Steve J ^b   Keithly, Janet S ^a  

^a WADSWORTH CENTER   (United States)

^b KANSAS STATE UNIVERSITY   (United States)

Author keywords

Cryptosporidium parvum; Molecular analysis; Type I fatty acid synthase

Indexed keywords

ACYL CARRIER PROTEIN; ACYLTRANSFERASE; CERULENIN; FATTY ACID SYNTHASE; LIGASE; OXIDOREDUCTASE; SYNTHETASE; THIOLACTOMYCIN;

ARTICLE; CONTROLLED STUDY; CRYPTOSPORIDIOSIS; CRYPTOSPORIDIUM PARVUM; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME SUBUNIT; GENE EXPRESSION; GENE LOCATION; GENE TARGETING; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; ANIMALS; CERULENIN; CRYPTOSPORIDIUM PARVUM; FATTY ACID SYNTHETASE COMPLEX; FLUORESCENT ANTIBODY TECHNIQUE; GENES, PROTOZOAN; HUMANS; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, TERTIARY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; THIOPHENES;

CRYPTOSPORIDIUM PARVUM; MAMMALIA; PLASMODIUM FALCIPARUM; PROTOZOA; TOXOPLASMA GONDII;

EID: 0033977411     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(99)00183-8     Document Type: Article

References (36)

1. Smith S. The animal fatty acid synthase: one gene, one polypeptide, seven enzymes. FASEB J. 8:1994;1248-1259.
2. Rawlings M., Cronan Jr J.E. The gene encoding Escherichia coli acyl carrier protein lies within a cluster of fatty acid biosynthetic genes. J. Biol. Chem. 267:1992;5751-5754.
3. Harwood J.L. Recent advances in the biosynthesis of plant fatty acids. Biochim. Biophys. Acta. 1301:1996;7-56.
4. Waller R.F., Keeling P.J., Donald R.G.K. et al. Nuclear-encoded proteins target to the plastid in Toxoplasma gondii and Plasmodium falciparum. Proc. Natl. Acad. Sci. USA. 95:1998;12352-12357.
5. Gardner M.J., Tettelin H., Carucci D.J. et al. Chromsome 2 sequence of the human malaria parasite Plasmodium faciparum. Science. 282:1998;1126-1132.
6. Taghi-Kilani R., Sekla L. Purification of Cryptosporidium parvum oocysts and sporozoites by cesium chloride and percoll gradients. Am. J. Trop. Med. Gyg. 36:1987;505-508.
7. Zhu G., Keithly J.S. Molecular analysis of a P-type ATPase from Cryptosporidium parvum. Mol. Biochem. Parasitol. 90:1997;307-316.
8. Nesterenko M.V., Upton S.J. A rapid microcentrifuge procedure for purification of Cryptosporidium sporozoites. J. Microbiol. Methods. 25:1996;87-89.
9. Funabashi H., Kawaguchi A., Tomoda H. et al. Binding site of cerulenin in fatty acid synthetase. J. Biochem. 105:1989;751-755.
10. Arimura N., Kaneda T. Type selective inhibition of microbial fatty acid synthases by thiolactomycin. Arch. Microbiol. 160:1993;158-161.
11. Upton S.J., Tilley M., Brillhart D.B. Effects of select medium supplements on in vitro development of Cryptosporidium parvum in HCT-8 cells. J. Clin. Microbiol. 33:1995;371-375.
12. Woods K.M., Nesterenko M.V., Upton S.J. Development of a microtitre ELISA to quantify development of Cryptosporidium parvum in vitro. FEMS Microbiol. Lett. 128:1995;89-94.
13. Woods K.M., Nesterenko M.V., Upton S.J. Efficacy of 101 antimicrobials and other agents on the development of Cryptosporidium parvum in vitro. Ann. Trop. Med. Parasitol. 90:1996;603-615.
14. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods. 65:1983;55-63.
15. Vistica D.T., Skehan P., Scudiero D. et al. Tetrazolium-based assays for cellular viability: a critical examination of selected parameters affecting formazan production. Cancer Res. 51:1991;2515-2520.
16. Blunt D.S., Khramtsov N.V., Upton S.J., Montelone B.A. Molecular karyotype analysis of Cryptosporidium parvum - evidence for eight chromosomes and a low-molecular-size molecule. Clin. Diag. Lab. Immunol. 4:1997;11-13.
17. Zhu G., Marchewka M.J., Keithly J.S. Cryptosporidium parvum possesses a short-type replication protein a large subunit that differs from its host. FEMS Microbiol. Lett. 176:1999;367-372.
18. Donadio S., Katz L. Organization of the enzymatic domains in the multifunctional polyketide synthase involved in erythromycin formation in Saccharopolyspora erythraea. Gene. 111:1992;51-60.
19. Aparicio J.F., Molnar I., Schwecke T. et al. Organization of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: analysis of the enzymatic domains in the modular polyketide synthase. Gene. 169:1996;9-16.
20. Amy C.M., Witkowski A., Naggert J. et al. Molecular cloning and sequencing of cDNAs encoding the entire rat fatty acid synthase. Proc. Natl. Acad. Sci. USA. 86:1989;3114-3118.
21. Schweizer M., Roberts L.M., Holtke H.J. et al. The pentafunctional FAS1 gene of yeast: its nucleotide sequence and order of the catalytic domains. Mol. Gen. Genet. 203:1986;479-486.
22. Wilson R.J., Williamson D.H. Extrachromosomal DNA in the Apicomplexa. Microbiol. Mol. Biol. Rev. 61:1997;1-16.
23. Zhu G, Marchewka MJ, Keithly JS. Cryptosporidium parvum appears to lack a plastid genome, Microbiology 1999; (in press).
24. Keithly J.S., Zhu G., Upton S.J. et al. Polyamine biosynthesis in Cryptosporidium parvum and its implications for chemotherapy. Mol. Biochem. Parasitol. 88:1997;35-42.
25. Coombs G.H., Denton H., Brown S.M., Thong K.W. Biochemistry of the coccidia. Adv. Parasitol. 39:1997;141-226.
26. Van de Peer Y., De Wachter R. Evolutionary relationships among the eukaryotic crown taxa taking into account site-to-site rate variation in 18S rRNA. J. Mol. Evol. 45:1997;619-630.
27. Lingelbach K., Joiner K.A. The parasitophorous vacuole membrane surrounding Plasmodium and Toxoplasma: an unusual compartment in infected cells. J. Cell Sci. 111:1998;1467-1475.
28. Broedel S.E.J., Zhao X.J., Cihlar R.L. Fatty acid synthase as a target in the development of new antifungals. Rec. Res. Devel. Antimicrob. Agents Chemother. 1:1996;25-33.
29. Zhao X.J., McElhaney-Feser G.E., Bowen W.H. et al. Requirement for the Candida. albicans FAS2 gene for infection in a rat model of oropharyngeal candidiasis. Microbiology. 142:1996;2509-2514.
30. Pizer E.S., Jackisch C., Wood F.D. et al. Inhibition of fatty acid synthesis induces programmed cell death in human breast cancer cells. Cancer Res. 56:1996;2745-2747.
31. Pizer E.S., Chrest F.J., DiGiuseppe J.A., Han W.F. Pharmacological inhibitors of mammalian fatty acid synthase suppress DNA replication and induce apoptosis in tumor cell lines. Cancer Res. 58:1998;4611-4615.
32. Baldock C., de Boer G.J., Rafferty J.B., Stuitje A.R., Rice D.W. Mechanism of action of diazaborines. Biochem. Pharmacol. 55:1998;1541-1549.
33. Mdluli K., Slayden R.A., Zhu Y. et al. Inhibition of a Mycobacterium tuberculosis β-ketoacyl ACP synthase by isoniazid. Science. 280:1998;1607-1610.
34. Heath R.J., Yu Y.T., Shapiro M.A., Olson E., Rock C.O. Broad spectrum antimicrobial biocides target the FabI component of fatty acid synthesis. J. Biol. Chem. 273:1998;30316-30320.
35. Heath R.J., Rubin J.R., Holland D.R. et al. Mechanism of triclosan inhibition of bacterial fatty acid synthesis. J. Biol. Chem. 274:1999;11110-11114.
36. McMurry L.M., McDermott P.F., Levy S.B. Genetic evidence that InhA of Mycobacterium smegmatis is a target for triclosan. Antimicrob. Agents Chemother. 43:1999;711-713.