A discussion of molecular biology methods for protein engineering

Molecular Biotechnology

Volumn 51, Issue 1, 2012, Pages 67-102

  Zawaira, Alexander ^a   Pooran, Anil ^b   Barichievy, Samantha ^a   Chopera, Denis ^c  

^a DSI CSIR Nanotechnology Innovation Centre   (South Africa)

^b UNIVERSITY OF CAPE TOWN   (South Africa)

^c UNIVERSITY OF CAPE TOWN   (South Africa)

Author keywords

Deletion mutagenesis; Insertion mutagenesis; Molecular biology; Molecular cloning; Mutagenesis; Polymerase chain reaction; Protein engineering; Recombinant plasmid; Recombinant protein expression; Site directed mutagenesis

Indexed keywords

DELETION MUTAGENESIS; MOLECULAR CLONING; PROTEIN ENGINEERING; RECOMBINANT PLASMID; RECOMBINANT PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS;

BIOMOLECULES; BIOPHYSICS; CLONING; GENE EXPRESSION; MOLECULAR BIOLOGY; MOLECULAR STRUCTURE; MUTAGENESIS; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS;

BIOCHEMICAL ENGINEERING;

AURORA B KINASE; DNA POLYMERASE; DOUBLE STRANDED DNA; EXONUCLEASE; GLUTATHIONE TRANSFERASE; INTERLEUKIN 4; METHYLTRANSFERASE; NUCLEOTIDYLTRANSFERASE; PLASMID DNA;

AGAR GEL ELECTROPHORESIS; ESCHERICHIA COLI; INVERSE POLYMERASE CHAIN REACTION; LUNG NON SMALL CELL CANCER; MOLECULAR BIOLOGY; MOLECULAR CLONING; MUTAGENESIS; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PLASMODIUM FALCIPARUM; PROTEIN ENGINEERING; REVIEW; TH2 CELL;

BASE SEQUENCE; DNA PRIMERS; MOLECULAR BIOLOGY; MOLECULAR SEQUENCE DATA; MUTAGENESIS; POLYMERASE CHAIN REACTION; PROTEIN ENGINEERING;

EID: 84859428492     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-011-9448-9     Document Type: Review

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