Megaprimer mutagenesis using very long primers

BioTechniques

Volumn 34, Issue 1, 2003, Pages 52-56

  Lai, R ^a   Bekessy, A ^a   Chen, C C ^a   Walsh, T ^b   Barnard, Ross ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b QUEENSLAND UNIVERSITY OF TECHNOLOGY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

MAGNESIUM CHLORIDE; PRIMER DNA;

ANALYTIC METHOD; ARTICLE; DNA BINDING; DNA TEMPLATE; GENE AMPLIFICATION; POLYMERASE CHAIN REACTION; SITE DIRECTED MUTAGENESIS;

EID: 12244290342     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/03341bm07     Document Type: Article

References (7)

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2. Colosimo, A., Z. Xu, G. Novelli, B. Dallapiccola, and D.C. Gruenert. 1999. Simple version of "megaprimer" PCR for site-directed mutagenesis. BioTechniques 26:870-873.
3. Datta, A.K. 1995. Efficient amplification using "megaprimer" by asymmetric polymerase chain reaction. Nucleic Acids Res. 23:4530-4531.
4. Giebel, L.B. and R.A. Spritz. 1990. Site-directed mutagenesis using a double-stranded DNA fragment as a PCR primer. Nucleic Acids Res. 25:4947.
5. Ling, M.M. and B.H. Robinson. 1997. Approaches to DNA mutagenesis: an overview. Anal. Biochem. 254:157-178.
6. Sarkar, G. and S.S. Sommer. 1990. The "megaprimer" method of site-directed mutagenesis. BioTechniques 8:404-407.
7. Smith, A.M. and K.P. Klugman. "Megaprimer" method of PCR-based mutagenesis: the concentration of megaprimer is a critical factor. BioTechniques 22:438-442.