Fluorometric detection of protein kinase Cα activity based on phosphorylation-induced dissociation of a polyion complex

Analytical Biochemistry

Volumn 424, Issue 2, 2012, Pages 130-136

  Toita, Riki ^a,b   Mori, Takeshi ^a   Naritomi, Yuki ^a   Kang, Jeong Hun ^a,c   Shiosaki, Shujiro ^a   Niidome, Takuro ^a   Katayama, Yoshiki ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b KYUSHU UNIVERSITY   (Japan)

^c NATIONAL CEREBRAL AND CARDIOVASCULAR CENTER   (Japan)

Author keywords

Cell based assay; Fluorometric detection; Polyion complex; Protein kinase

Indexed keywords

DISSOCIATION; ENERGY TRANSFER; ENZYMES; FLUORESCENCE QUENCHING; INFRARED DEVICES; PEPTIDES; PHOSPHORYLATION; SULFUR COMPOUNDS;

CELL-BASED ASSAYS; CHONDROITIN SULFATES; CONCENTRATION-DEPENDENT MANNERS; FLUORESCENCE INTENSITIES; FLUOROMETRIC DETECTION; POLYION COMPLEX; PROTEIN KINASE; RESONANCE ENERGY TRANSFER;

FLUORESCENCE;

CHONDROITIN SULFATE; DENDRIMER; PROTEIN KINASE C ALPHA;

ARTICLE; ENZYME ACTIVITY; ENZYME PHOSPHORYLATION; ENZYME PURIFICATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUOROMETRY; INFRARED SPECTROSCOPY; PRIORITY JOURNAL;

EID: 84859042294     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2012.01.036     Document Type: Article

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