A multiplexed homogeneous fluorescence-based assay for protein kinase activity in cell lysates

Nature Methods

Volumn 2, Issue 4, 2005, Pages 277-283

  Shults, Melissa D ^a   Janes, Kevin A ^a   Lauffenburger, Douglas A ^a   Imperiali, Barbara ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL EXTRACT; CYCLIC AMP DEPENDENT PROTEIN KINASE; MITOGEN ACTIVATED PROTEIN KINASE KINASE 2; PROTEIN KINASE; PROTEIN KINASE B; RECOMBINANT ENZYME;

ARTICLE; CELL ACTIVITY; CELL FRACTIONATION; CELL LYSATE; CHEMOSENSITIVITY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME PHOSPHORYLATION; FLUORESCENCE ANALYSIS; HUMAN; HUMAN CELL; LINEAR SYSTEM; MAMMAL CELL; PRIORITY JOURNAL; PROCESS DESIGN; PROCESS DEVELOPMENT; PROCESS MONITORING; PROTEIN TARGETING; REPORTER GENE; REPRODUCIBILITY; SENSOR; SIGNAL TRANSDUCTION; CELL FREE SYSTEM; CELL LINE; ENZYME ACTIVATION; ENZYMOLOGY; EPITHELIUM CELL; EVALUATION; GENETIC PROCEDURES; METABOLISM; METHODOLOGY; SPECTROFLUOROMETRY;

MAMMALIA;

BIOSENSING TECHNIQUES; CELL EXTRACTS; CELL LINE; CELL-FREE SYSTEM; ENZYME ACTIVATION; EPITHELIAL CELLS; HUMANS; PROTEIN KINASES; SPECTROMETRY, FLUORESCENCE;

EID: 18744408814     PISSN: 15487091     EISSN: None     Source Type: Journal    
DOI: 10.1038/nmeth747     Document Type: Article

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