An evaluation of fluorescence polarization and lifetime discriminated polarization for high throughput screening of serine/threonine kinases

Analytical Biochemistry

Volumn 308, Issue 2, 2002, Pages 223-231

  Fowler, Ann ^a,b   Swift, Denise ^a   Longman, Emma ^a   Acornley, Anne ^a   Hemsley, Paul ^a   Murray, Dave ^a   Unitt, John ^a   Dale, Ian ^a   Sullivan, Elaine ^a   Coldwell, Martin ^a  

^a ASTRAZENECA   (United Kingdom)

^b F HOFFMANN LA ROCHE LTD   (Switzerland)

Author keywords

FLARe; Fluorescence lifetime; Fluorescence polarization; HTS; Kinases

Indexed keywords

AMINO ACIDS; ENZYME KINETICS; EXCITED STATES; FLUOROPHORES; POLARIZATION;

C-JUN N-TERMINAL KINASE; COMPOUND INTERFERENCE; FLARE; FLUORESCENCE LIFETIMES; FLUORESCENCE POLARIZATION; FLUORESCENT COMPOUNDS; HIGH THROUGHPUT SCREENING; KINASES;

FLUORESCENCE;

ADENOSINE TRIPHOSPHATE; PROTEIN KINASE C; PROTEIN SERINE THREONINE KINASE; STRESS ACTIVATED PROTEIN KINASE;

ARTICLE; ASSAY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME KINETICS; FLUORESCENCE; FLUORESCENCE POLARIZATION; IC 50; INSTRUMENT; LIFESPAN; PRIORITY JOURNAL; SCREENING;

EID: 18644384952     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2697(02)00245-2     Document Type: Article

References (6)

1. T. French, J.C. Owicki, D.N. Modlin, S.S. Deshpande, I. Mineyev, K. Crawford, W. Burton, Fluorescence-lifetime technologies for high-throughput screening, Proc. SPIE 3259 (1998) 209-218.
2. T. French, B. Bailey, D.P. Stumbo, D.N. Modlin, A time-resolved fluorometer for high-throughput screening, Proc. SPIE 3603 (1999) 272-280.
3. Y. Fleming, C.G. Armstrong, N. Morrice, A. Paterson, M. Goedert, P. Cohen, Synergistic activation of stress-activated protein kinase 1/c-Jun N-terminal kinase (SAPK1/JNK) isoforms by mitogen-activated protein kinase kinase 4 (MKK4) and MKK7, Biochem. 352 (2000) 145-154.
4. J.H. Zhang, T.D.Y. Chung, K.R. Oldenburg, A simple statistical parameter for use in evaluation and validation of high throughput screening assays, J. Biomol. Screening 4 (1999) 67-73.
5. G.R. Fleming, A.W.E. Knight, J.M. Morris, R.J.S. Morrison, G.W. Robinson, Picosecond fluorescence studies of xanthene dyes, J. Am. Chem. Soc. 99 (1977) 4306-4311.
6. N. Periasamy, A.S. Verkman, Subtraction of background fluorescence in multiharmonic frequency-domain fluorimetry, Anal. Biochem. 201 (1992) 107-113.