Design, modeling, and expression of erythropoietin cysteine analogs in Pichia pastoris: Improvement of mean residence times and in vivo activities through cysteine-specific PEGylation

European Journal of Pharmaceutics and Biopharmaceutics

Volumn 80, Issue 3, 2012, Pages 499-507

  Maleki, Ahmad ^a,b   Madadkar Sobhani, Armin ^c,d   Roohvand, Farzin ^b   Najafabadi, Abdolhossein R ^a   Shafiee, Abbas ^a   Khanahmad, Hossein ^b   Cohan, Reza A ^b   Namvar, Nabiallah ^b   Tajerzadeh, Hosnieh ^a  

^a TEHRAN UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^b PASTEUR INSTITUTE OF IRAN   (Iran)

^c UNIVERSITY OF TEHRAN   (Iran)

^d BARCELONA SUPERCOMPUTING CENTER   (Spain)

Author keywords

Bioactivity; Cysteine specific PEGylation; Erythropoietin cysteine analogs; Pharmacokinetics; Pichia pastoris

Indexed keywords

COMPLEMENTARY DNA; CYSTEINE; MACROGOL; MALEIMIDE DERIVATIVE; METHOXYPOLYETHYLENE GLYCOL; PROPYLENE GLYCOL; RECOMBINANT ERYTHROPOIETIN; RECOMBINANT ERYTHROPOIETIN CYSTEINE DERIVATIVE; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; AREA UNDER THE CURVE; ARTICLE; CELL PROLIFERATION; CHEMICAL MODIFICATION; CONJUGATION; CONTROLLED STUDY; COST CONTROL; CULTURE OPTIMIZATION; DRUG ACTIVITY; DRUG BLOOD LEVEL; DRUG CLEARANCE; DRUG CONFORMATION; DRUG DISTRIBUTION; DRUG DOSAGE FORM COMPARISON; DRUG HALF LIFE; DRUG STRUCTURE; EXPERIMENTAL RABBIT; EXPRESSION VECTOR; FUNGUS CULTURE; IN VIVO STUDY; MALE; MEAN RESIDENCE TIME; MOLECULAR CLONING; MOLECULAR DYNAMICS; MOLECULAR MODEL; MOLECULAR WEIGHT; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PEGYLATION; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN SECRETION; PROTEIN STABILITY; SIMULATION; SITE DIRECTED MUTAGENESIS; STRUCTURAL HOMOLOGY;

ANIMALS; CELL LINE, TUMOR; COMPUTATIONAL BIOLOGY; CYSTEINE; DRUG DESIGN; ERYTHROPOIETIN; GENETIC VECTORS; HEMATINICS; HUMANS; MALE; MALEIMIDES; MICE; MOLECULAR DYNAMICS SIMULATION; MUTAGENESIS, SITE-DIRECTED; PICHIA; POLYETHYLENE GLYCOLS; RABBITS; RECOMBINANT PROTEINS;

EID: 84858150416     PISSN: 09396411     EISSN: 18733441     Source Type: Journal    
DOI: 10.1016/j.ejpb.2011.10.017     Document Type: Article

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