메뉴 건너뛰기




Volumn 8, Issue 1, 2012, Pages 14-20

Pyrosequencing-an alternative to traditional Sanger sequencing

Author keywords

Advantage; Alternative; Pyrosequencing; Sanger sequencing

Indexed keywords

DNA; DNA SEQUENCES; ELECTROPHORESIS; NUCLEIC ACID SEQUENCES; NUCLEIC ACIDS; NUCLEOTIDES;

EID: 84857949441     PISSN: 15533468     EISSN: None     Source Type: Journal    
DOI: 10.3844/ajbbsp.2012.14.20     Document Type: Review
Times cited : (47)

References (51)
  • 1
    • 15944386270 scopus 로고    scopus 로고
    • Simultaneous detection of herpes simplex virus types 1 and 2 by real-time PCR and pyrosequencing
    • Adelson, M.E., M. Feola, J. Trama, R.C. Tilton and E. Mordechai, 2005. Simultaneous detection of herpes simplex virus types 1 and 2 by real-time PCR and pyrosequencing. J. Clin. Virol., 33: 25-34.
    • (2005) J. Clin. Virol. , vol.33 , pp. 25-34
    • Adelson, M.E.1    Feola, M.2    Trama, J.3    Tilton, R.C.4    Mordechai, E.5
  • 4
    • 67349209853 scopus 로고    scopus 로고
    • Next-generation DNA sequencing Techniques
    • Ansorge, W.J., 2009. Next-generation DNA sequencing Techniques. New Biotechnol., 25: 195-203.
    • (2009) New Biotechnol , vol.25 , pp. 195-203
    • Ansorge, W.J.1
  • 5
    • 0024263226 scopus 로고
    • A novel method for nucleic acid sequence determination
    • DOI: 10.1016/S0022-5193(88)80246-7
    • Bains, W and G.C. Smith, 1988. A novel method for nucleic acid sequence determination. J. Theoretical Biol., 135: 303-307. DOI: 10.1016/S0022-5193(88)80246-7
    • (1988) J. Theoretical Biol. , vol.135 , pp. 303-307
    • Bains, W.1    Smith, G.C.2
  • 6
    • 0028829315 scopus 로고
    • Kinetic analysis of nucleotide incorporation and misincorporation by Klenow fragment of Escherichia coli DNA polymerase I
    • DOI: 10.1016/0076-6879(95)62022-2
    • Benkovic, S.J. and C.E. Cameron, 1995. Kinetic analysis of nucleotide incorporation and misincorporation by Klenow fragment of Escherichia coli DNA polymerase I. Methods Enzymol., 262: 257-269. DOI: 10.1016/0076-6879(95)62022-2
    • (1995) Methods Enzymol , vol.262 , pp. 257-269
    • Benkovic, S.J.1    Cameron, C.E.2
  • 7
    • 12944293179 scopus 로고    scopus 로고
    • In vitro cloning of complex mixtures of DNA on microbeads: Physical separation of differentially expressed cDNAs
    • DOI: 10.1073/pnas.97.4.1665
    • Brenner, S., S.R. Williams, E.H. Vermaas, T. Storck and K. Moon et al., 2000. In vitro cloning of complex mixtures of DNA on microbeads: Physical separation of differentially expressed cDNAs. Proc. Natl. Acad. Sci., 97: 1665-1670. DOI: 10.1073/pnas.97.4.1665
    • (2000) Proc. Natl. Acad. Sci. , vol.97 , pp. 1665-1670
    • Brenner, S.1    Williams, S.R.2    Vermaas, E.H.3    Storck, T.4    Moon, K.5
  • 8
    • 0024615295 scopus 로고
    • Sequencing of megabase plus DNA by hybridization: Theory of the method
    • Doi: 10.1016/0888-7543(89)90290-5
    • Drmanac, R., I. Labat, I. Brukner and R. Crkvenjakov, 1989. Sequencing of megabase plus DNA by hybridization: Theory of the method. Genomics, 4: 114-128. Doi: 10.1016/0888-7543(89)90290-5
    • (1989) Genomics , vol.4 , pp. 114-128
    • Drmanac, R.1    Labat, I.2    Brukner, I.3    Crkvenjakov, R.4
  • 9
    • 0037403289 scopus 로고    scopus 로고
    • Differences in nucleotide-binding site of isoapyrases deduced from tryptophan fluorescence
    • Espinosa, V., A.M. Kettlun, A. Zanocco, E. Cardemil and M.A. Valenzuela, 2003. Differences in nucleotide-binding site of isoapyrases deduced from tryptophan fluorescence. Photochemistry, 63: 7-14.
    • (2003) Photochemistry , vol.63 , pp. 7-14
    • Espinosa, V.1    Kettlun, A.M.2    Zanocco, A.3    Cardemil, E.4    Valenzuela, M.A.5
  • 10
    • 0034622620 scopus 로고    scopus 로고
    • Mutation detection by pyrosequencing: Sequencing of exons 5-8 of the p53 tumour suppressor gene
    • Garcia, C.A., A. Ahmadian, B. Gharizadeh, J. Lundeberg and M. Ronaghi et al., 2000. Mutation detection by pyrosequencing: Sequencing of exons 5-8 of the p53 tumour suppressor gene. Gene, 253: 249-257.
    • (2000) Gene , vol.253 , pp. 249-257
    • Garcia, C.A.1    Ahmadian, A.2    Gharizadeh, B.3    Lundeberg, J.4    Ronaghi, M.5
  • 12
    • 0036467709 scopus 로고    scopus 로고
    • Long-read pyrosequencing using pure 2'-deoxyadenosine-5'-O'-(1-thiotriphosphate) Spisomer
    • Anal Biochem. 2002
    • Gharizadeh, B., T. Nordstrom, A. Ahmadian, M. Ronaghi and P. Nyren, 2002. Anal Biochem. 2002. Long-read pyrosequencing using pure 2'-deoxyadenosine-5'-O'-(1-thiotriphosphate) Spisomer. Anal. Biochem., 301: 82-90.
    • (2002) Anal. Biochem. , vol.301 , pp. 82-90
    • Gharizadeh, B.1    Nordstrom, T.2    Ahmadian, A.3    Ronaghi, M.4    Nyren, P.5
  • 14
    • 2142744331 scopus 로고    scopus 로고
    • Viral and microbial genotyping by a combination of multiplex competitive hybridization and specific extension followed by hybridization to generic tag arrays
    • DOI: 10.1093/nar/gng147
    • Gharizadeh, B., M. Kaller, P. Nyren, A. Andersson and M. Uhlen et al., 2003a. Viral and microbial genotyping by a combination of multiplex competitive hybridization and specific extension followed by hybridization to generic tag arrays. Nucl. Acids Res., 31: 146-146. DOI: 10.1093/nar/gng147
    • (2003) Nucl. Acids Res. , vol.31 , pp. 146
    • Gharizadeh, B.1    Kaller, M.2    Nyren, P.3    Andersson, A.4    Uhlen, M.5
  • 15
    • 0043237239 scopus 로고    scopus 로고
    • Multiple group-specific sequencing primers for reliable and rapid DNA sequencing
    • Gharizadeh, B., A. Ohlin, P. Molling, A. Backman and B. Amini et al., 2003b. Multiple group-specific sequencing primers for reliable and rapid DNA sequencing. Mol. Cell Probes, 17: 203-210.
    • (2003) Mol. Cell Probes. , vol.17 , pp. 203-210
    • Gharizadeh, B.1    Ohlin, A.2    Molling, P.3    Backman, A.4    Amini, B.5
  • 16
    • 0347134737 scopus 로고    scopus 로고
    • Multiple-primer DNA sequencing method
    • DOI: 10.1002/elps.200390147
    • Gharizadeh, B., M. Ghaderi, D. Donnelly, B. Amini and K.L. Wallin et al., 2003c. Multiple-primer DNA sequencing method. Electrophoresis, 24: 1145-1151. DOI: 10.1002/elps.200390147
    • (2003) Electrophoresis , vol.24 , pp. 1145-1151
    • Gharizadeh, B.1    Ghaderi, M.2    Donnelly, D.3    Amini, B.4    Wallin, K.L.5
  • 17
    • 2942525596 scopus 로고    scopus 로고
    • Improvements in pyrosequencing technology by employing sequenase polymerase
    • DOI: 10.1016/j.ab.2004.03.018
    • Gharizadeh, B., J. Eriksson, N. Nourizad, T. Nordstrom and P. Nyren, 2004. Improvements in pyrosequencing technology by employing sequenase polymerase. Anal. Biochem., 330: 272-280. DOI: 10.1016/j.ab.2004.03.018
    • (2004) Anal. Biochem. , vol.330 , pp. 272-280
    • Gharizadeh, B.1    Eriksson, J.2    Nourizad, N.3    Nordstrom, T.4    Nyren, P.5
  • 18
    • 18744396366 scopus 로고    scopus 로고
    • Type-specific multiple sequencing primers: A novel strategy for reliable and rapid genotyping of human papillomaviruses by pyrosequencing technology
    • Gharizadeh, B., M. Oggionni, B. Zheng, E. Akom and N. Pourmand, 2005. Type-specific multiple sequencing primers: A novel strategy for reliable and rapid genotyping of human papillomaviruses by pyrosequencing technology. J. Mol. Diagn., 7: 198-205.
    • (2005) J. Mol. Diagn. , vol.7 , pp. 198-205
    • Gharizadeh, B.1    Oggionni, M.2    Zheng, B.3    Akom, E.4    Pourmand, N.5
  • 19
    • 33646252262 scopus 로고    scopus 로고
    • Sentinel-base DNA genotyping using multiple sequencing primers for high-risk human papillomaviruses
    • DOI: 10.1016/j.mcp.2006.01.002
    • Gharizadeh, B., B. Zheng, M. Akhras, M. Ghaderi and O. Jejelowo, 2006. Sentinel-base DNA genotyping using multiple sequencing primers for high-risk human papillomaviruses. Mol. Cell Probes, 20: 230-238. DOI: 10.1016/j.mcp.2006.01.002
    • (2006) Mol. Cell Probes. , vol.20 , pp. 230-238
    • Gharizadeh, B.1    Zheng, B.2    Akhras, M.3    Ghaderi, M.4    Jejelowo, O.5
  • 20
    • 84857929318 scopus 로고    scopus 로고
    • Pyrosequencing technology for short DNA sequencing and whole genome sequencing
    • DOI: 10.2142/biophys.47.129
    • Gharizadeh, B., M. Ghaderi and P. Nyren, 2007. Pyrosequencing technology for short DNA sequencing and whole genome sequencing. Tech., 47: 129-132. DOI: 10.2142/biophys.47.129
    • (2007) Tech , vol.47 , pp. 129-132
    • Gharizadeh, B.1    Ghaderi, M.2    Nyren, P.3
  • 21
    • 0037436159 scopus 로고    scopus 로고
    • Identification of mixed bacterial DNA contamination in broad-range PCR amplification of 16S rDNA V1 and V3 variable regions by pyrosequencing of cloned amplicons
    • DOI: 10.1016/S0378-1097(02)01190-4
    • Grahn, N., M. Olofsson, K. Ellnebo-Svedlund, H.J. Monstein and J. Jonasson, 2003. Identification of mixed bacterial DNA contamination in broad-range PCR amplification of 16S rDNA V1 and V3 variable regions by pyrosequencing of cloned amplicons. FEMS Microbiol. Lett., 219: 87-91. DOI: 10.1016/S0378-1097(02)01190-4
    • (2003) FEMS Microbiol. Lett. , vol.219 , pp. 87-91
    • Grahn, N.1    Olofsson, M.2    Ellnebo-Svedlund, K.3    Monstein, H.J.4    Jonasson, J.5
  • 22
    • 34249723655 scopus 로고    scopus 로고
    • Advanced sequencing technologies and their wider impact in microbiology
    • DOI: 10.1242/ jeb.001370
    • Hall, N., 2007. Advanced sequencing technologies and their wider impact in microbiology. J. Exp. Biol., 209: 1518-1525. DOI: 10.1242/ jeb.001370
    • (2007) J. Exp. Biol. , vol.209 , pp. 1518-1525
    • Hall, N.1
  • 23
    • 0023715281 scopus 로고
    • A new method of sequencing DNA
    • DOI: 10.1016/0003-2697(88)90041-3
    • Hyman, E.D., 1988. A new method of sequencing DNA. Anal. Biochem., 174: 423-436. DOI: 10.1016/0003-2697(88)90041-3
    • (1988) Anal. Biochem. , vol.174 , pp. 423-436
    • Hyman, E.D.1
  • 24
    • 0036240638 scopus 로고    scopus 로고
    • Classification, identification and subtyping of bacteria based on pyrosequencing and signature matching of 16S rDNA fragments
    • DOI: 10.1034/j.1600-0463.2002.100309.x
    • Jonasson, J., M. Olofsson and H.J. Monstein, 2002. Classification, identification and subtyping of bacteria based on pyrosequencing and signature matching of 16S rDNA fragments. APMIS, 110: 263-272. DOI: 10.1034/j.1600-0463.2002.100309.x
    • (2002) APMIS , vol.110 , pp. 263-272
    • Jonasson, J.1    Olofsson, M.2    Monstein, H.J.3
  • 25
    • 0033117544 scopus 로고    scopus 로고
    • Production, purification and luminometric analysis of recombinant Saccharomyces cerevisiae MET3 adenosine triphosphate sulfurylase expressed in Escherichia coli
    • Karamohamed, S., J. Nilsson, K. Nourizad, M. Ronaghi and B. Pettersson et al., 1999. Production, purification and luminometric analysis of recombinant Saccharomyces cerevisiae MET3 adenosine triphosphate sulfurylase expressed in Escherichia coli. Protein Exp. Purif., 15: 381-388.
    • (1999) Protein Exp. Purif. , vol.15 , pp. 381-388
    • Karamohamed, S.1    Nilsson, J.2    Nourizad, K.3    Ronaghi, M.4    Pettersson, B.5
  • 26
    • 0024441819 scopus 로고
    • An oligonucleotide hybridization approach to DNA sequencing
    • DOI: 10.1016/0014-5793(89)81730-2
    • Khrapko, K.R., Y.P. Lysov, A.A. Khorlyn, V.V. Shick and V.L. Florentiev et al., 1989. An oligonucleotide hybridization approach to DNA sequencing. FEBS Lett., 256: 118-122. DOI: 10.1016/0014-5793(89)81730-2
    • (1989) FEBS Lett , vol.256 , pp. 118-122
    • Khrapko, K.R.1    Lysov, Y.P.2    Khorlyn, A.A.3    Shick, V.V.4    Florentiev, V.L.5
  • 28
    • 0037809252 scopus 로고    scopus 로고
    • The NudA protein in the gastric pathogen Helicobacter pylori is an ubiquitous and constitutively expressed dinucleoside polyphosphate hydrolase
    • DOI: 10.1074/jbc.M212542200
    • Lundin, A., C. Nilsson, M. Gerhard, D.I. Andersson and M. Krabbe et al., 2003. The NudA protein in the gastric pathogen Helicobacter pylori is an ubiquitous and constitutively expressed dinucleoside polyphosphate hydrolase. J. Biol. Chem., 278: 12574-12578. DOI: 10.1074/jbc.M212542200
    • (2003) J. Biol. Chem. , vol.278 , pp. 12574-12578
    • Lundin, A.1    Nilsson, C.2    Gerhard, M.3    Andersson, D.I.4    Krabbe, M.5
  • 29
    • 24044455869 scopus 로고    scopus 로고
    • Genome sequencing in microfabricated high-density picolitre reactors
    • DOI: 10.1038/nature03959
    • Margulies, M.M., M. Egholm, W.E. Altman, S. Attiya and J.S. Bader et al., 2005. Genome sequencing in microfabricated high-density picolitre reactors. Nature, 437: 376-380. DOI: 10.1038/nature03959
    • (2005) Nature , vol.437 , pp. 376-380
    • Margulies, M.M.1    Egholm, M.2    Altman, W.E.3    Attiya, S.4    Bader, J.S.5
  • 31
    • 0034685949 scopus 로고    scopus 로고
    • A mutation in PRKAG3 associated with excess glycogen content in Pig Skeletal Muscle
    • DOI: 10.1126/science.288.5469.1248
    • Milan, D., J.T. Jeon, C. Looft, V. Amarger and A. Robic, 2000. A mutation in PRKAG3 associated with excess glycogen content in Pig Skeletal Muscle. Science, 288: 1248-1251. DOI: 10.1126/science.288.5469.1248
    • (2000) Science , vol.288 , pp. 1248-1251
    • Milan, D.1    Jeon, J.T.2    Looft, C.3    Amarger, V.4    Robic, A.5
  • 32
    • 0035873176 scopus 로고    scopus 로고
    • Rapid molecular identification and subtyping of Helicobacter pylori by pyrosequencing of the 16S rDNA variable V1 and V3 regions
    • DOI: 10.1016/S0378-1097(01)00171-9
    • Monstein, H.J., S. Nikpour-Badr and J. Jonasson, 2001. Rapid molecular identification and subtyping of Helicobacter pylori by pyrosequencing of the 16S rDNA variable V1 and V3 regions. FEMS Microbiol. Lett., 199: 103-107. DOI: 10.1016/S0378-1097(01)00171-9
    • (2001) FEMS Microbiol. Lett. , vol.199 , pp. 103-107
    • Monstein, H.J.1    Nikpour-Badr, S.2    Jonasson, J.3
  • 33
    • 0036244765 scopus 로고    scopus 로고
    • Rapid SNP allele frequency determination in genomic DNA pools by pyrosequencing
    • Neve, B., P. Froguel, L. Corset, E. Vaillant and V. Vatin et al., 2002. Rapid SNP allele frequency determination in genomic DNA pools by pyrosequencing. Biotechniques, 32: 1138-1142.
    • (2002) Biotechniques , vol.32 , pp. 1138-1142
    • Neve, B.1    Froguel, P.2    Corset, L.3    Vaillant, E.4    Vatin, V.5
  • 34
    • 0035873505 scopus 로고    scopus 로고
    • Method enabling fast partial sequencing of cDNA clones
    • DOI: 10.1006/abio.2001.5094
    • Nordstrom, T., B. Gharizadeh, N. Pourmand, P. Nyren and M. Ronaghi, 2001. Method enabling fast partial sequencing of cDNA clones. Anal. Biochem., 292: 266-271. DOI: 10.1006/abio.2001.5094
    • (2001) Anal. Biochem. , vol.292 , pp. 266-271
    • Nordstrom, T.1    Gharizadeh, B.2    Pourmand, N.3    Nyren, P.4    Ronaghi, M.5
  • 35
    • 0347369488 scopus 로고    scopus 로고
    • Direct analysis of single-nucleotide polymorphism on doublestranded DNA by pyrosequencing
    • DOI: 10.1042/BA19990104
    • Nordstrom, T., M. Ronaghi, L. Forsberg, U. Faire and R. Morgenstern et al., 2000. Direct analysis of single-nucleotide polymorphism on doublestranded DNA by pyrosequencing. Biotechnol. Applied Biochem., 31: 107-112. DOI: 10.1042/BA19990104
    • (2000) Biotechnol. Applied Biochem. , vol.31 , pp. 107-112
    • Nordstrom, T.1    Ronaghi, M.2    Forsberg, L.3    Faire, U.4    Morgenstern, R.5
  • 36
    • 0037296231 scopus 로고    scopus 로고
    • Methylotrophic yeast Pichia pastoris as a host for production of ATPdiphosphohydrolase (apyrase) from potato tubers (Solanum tuberosum)
    • Nourizad N., M. Ehn, B. Gharizadeh, S. Hober and P. Nyren, 2003. Methylotrophic yeast Pichia pastoris as a host for production of ATPdiphosphohydrolase (apyrase) from potato tubers (Solanum tuberosum). Protein Exp. Purif., 27: 229-237.
    • (2003) Protein Exp. Purif. , vol.27 , pp. 229-237
    • Nourizad, N.1    Ehn, M.2    Gharizadeh, B.3    Hober, S.4    Nyren, P.5
  • 37
    • 0022313129 scopus 로고
    • Enzymatic method for continuous monitoring of inorganic pyrophosphate synthesis
    • DOI: 10.1016/0003-2697(85)90211-8
    • Nyren, P and A. Lundin, 1985. Enzymatic method for continuous monitoring of inorganic pyrophosphate synthesis. Anal. Biochem., 151: 504-509. DOI: 10.1016/0003-2697(85)90211-8
    • (1985) Anal. Biochem. , vol.151 , pp. 504-509
    • Nyren, P.1    Lundin, A.2
  • 39
    • 0035129508 scopus 로고    scopus 로고
    • Monitoring resistance to human immunodeficiency virus type 1 protease inhibitors by pyrosequencing
    • DOI: 10.1128/ JCM.39.2.464-473.2001
    • O'Meara, D., K. Wilbe, T. Leitner, B. Hejdeman and J. Albert et al., 2001. Monitoring resistance to human immunodeficiency virus type 1 protease inhibitors by pyrosequencing. J. Clin. Microbiol., 39: 464-473. DOI: 10.1128/ JCM.39.2.464-473.2001
    • (2001) J. Clin. Microbiol. , vol.39 , pp. 464-473
    • O'Meara, D.1    Wilbe, K.2    Leitner, T.3    Hejdeman, B.4    Albert, J.5
  • 40
    • 0034669368 scopus 로고    scopus 로고
    • Improved performance of pyrosequencing using single-stranded DNAbinding protein
    • Ronaghi, M., 2000. Improved performance of pyrosequencing using single-stranded DNAbinding protein. Anal. Biochem., 286: 282-288.
    • (2000) Anal. Biochem. , vol.286 , pp. 282-288
    • Ronaghi, M.1
  • 41
    • 0035153070 scopus 로고    scopus 로고
    • Pyrosequencing sheds light on DNA sequencing
    • DOI: 10.1101/gr.150601
    • Ronaghi, M., 2001. Pyrosequencing sheds light on DNA sequencing. Genome Res., 11: 3-11. DOI: 10.1101/gr.150601
    • (2001) Genome Res , vol.11 , pp. 3-11
    • Ronaghi, M.1
  • 42
    • 0033081736 scopus 로고    scopus 로고
    • Analyses of secondary structures in DNA by pyrosequencing
    • DOI: 10.1006/abio.1998.2978
    • Ronaghi, M., M. Nygren, J. Lundeberg and P. Nyren, 1999. Analyses of secondary structures in DNA by pyrosequencing. Anal. Biochem., 267: 65-71. DOI: 10.1006/abio.1998.2978
    • (1999) Anal. Biochem. , vol.267 , pp. 65-71
    • Ronaghi, M.1    Nygren, M.2    Lundeberg, J.3    Nyren, P.4
  • 43
    • 0032540905 scopus 로고    scopus 로고
    • A sequencing method based on real-time pyrophosphate
    • DOI: 10.1126/science.281.5375.363
    • Ronaghi, M., M. Uhlen and P. Nyren, 1998. A sequencing method based on real-time pyrophosphate. Sci., 281: 363-365. DOI: 10.1126/science.281.5375.363
    • (1998) Sci , vol.281 , pp. 363-365
    • Ronaghi, M.1    Uhlen, M.2    Nyren, P.3
  • 44
    • 0030298490 scopus 로고    scopus 로고
    • Real-time DNA sequencing using detection of pyrophosphate release
    • DOI: 10.1006/abio.1996.0432
    • Ronaghi, M., S. Karamohamed, B. Pettersson, M. Uhlen and P. Nyren, 1996. Real-time DNA sequencing using detection of pyrophosphate release. Anal. Biochem., 242: 84-89. DOI: 10.1006/abio.1996.0432
    • (1996) Anal. Biochem. , vol.242 , pp. 84-89
    • Ronaghi, M.1    Karamohamed, S.2    Pettersson, B.3    Uhlen, M.4    Nyren, P.5
  • 47
    • 0242416570 scopus 로고    scopus 로고
    • Profiling and identification of Eubacteria in the stomach of Mongolian gerbils with and without Helicobacter pylori infection
    • DOI: 10.1046/j.1523-5378.2003.00136.x
    • Sun, Y.Q., H.J. Monstein, L.E. Nilsson, F. Petersson and K. Borch, 2003. Profiling and identification of Eubacteria in the stomach of Mongolian gerbils with and without Helicobacter pylori infection. Helicobacter, 8: 149-157. DOI: 10.1046/j.1523-5378.2003.00136.x
    • (2003) Helicobacter , vol.8 , pp. 149-157
    • Sun, Y.Q.1    Monstein, H.J.2    Nilsson, L.E.3    Petersson, F.4    Borch, K.5
  • 48
    • 0036876247 scopus 로고    scopus 로고
    • Identification of randomly selected colonies of lactobacilli from normal vaginal fluid by pyrosequencing of the 16S rDNA variable V1 and V3 regions
    • Tarnberg, M., T. Jakobsson, J. Jonasson and U. Forsum, 2002. Identification of randomly selected colonies of lactobacilli from normal vaginal fluid by pyrosequencing of the 16S rDNA variable V1 and V3 regions. APMIS, 110: 801-810.
    • (2002) APMIS , vol.110 , pp. 801-810
    • Tarnberg, M.1    Jakobsson, T.2    Jonasson, J.3    Forsum, U.4
  • 49
    • 13844275378 scopus 로고    scopus 로고
    • Detection of Aspergillus fumigatus and a mutation that confers reduced susceptibility to itraconazole and posaconazole by real-time PCR and pyrosequencing
    • DOI: 10.1128/ JCM.43.2.906-908.2005
    • Trama, J.P., E. Mordechai and M.E. Adelson, 2005. Detection of Aspergillus fumigatus and a mutation that confers reduced susceptibility to itraconazole and posaconazole by real-time PCR and pyrosequencing. J. Clin. Microbiol., 43: 906-908. DOI: 10.1128/ JCM.43.2.906-908.2005
    • (2005) J. Clin. Microbiol. , vol.43 , pp. 906-908
    • Trama, J.P.1    Mordechai, E.2    Adelson, M.E.3
  • 50
    • 0035511962 scopus 로고    scopus 로고
    • Pyrosequencing as a method for grouping of Listeria monocytogenes strains on the basis of single-nucleotide polymorphisms in the inlB gene
    • DOI: 10.1128/AEM.67.11.5339-5342.2001a
    • Unnerstad, H., H. Ericsson, A. Alderborn, W. Tham and M.L. Danielsson-Tham et al., 2001. Pyrosequencing as a method for grouping of Listeria monocytogenes strains on the basis of single-nucleotide polymorphisms in the inlB gene. Applied Environ. Microbiol., 67: 5339-5342. DOI: 10.1128/AEM.67.11.5339-5342.2001a
    • (2001) Applied Environ. Microbiol. , vol.67 , pp. 5339-5342
    • Unnerstad, H.1    Ericsson, H.2    Alderborn, A.3    Tham, W.4    Danielsson-Tham, M.L.5
  • 51
    • 0036962761 scopus 로고    scopus 로고
    • Evaluation of a potential epigenetic biomarker by quantitative methyl-single nucleotide polymorphism analysis
    • DOI: 10.1002/elps.200290023
    • Uhlmann, K., A. Brinckmann, M.R. Toliat, H. Ritter and P. Nurnberg, 2002. Evaluation of a potential epigenetic biomarker by quantitative methyl-single nucleotide polymorphism analysis. Electrophoresis, 23: 4072-4079. DOI: 10.1002/elps.200290023
    • (2002) Electrophoresis , vol.23 , pp. 4072-4079
    • Uhlmann, K.1    Brinckmann, A.2    Toliat, M.R.3    Ritter, H.4    Nurnberg, P.5


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.