Improved performance of pyrosequencing using single-stranded DNA-binding protein

Analytical Biochemistry

Volumn 286, Issue 2, 2000, Pages 282-288

  Ronaghi, Mostafa ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Apyrase; ATP sulfurylase; Bioassay; DNA polymerase; DNA sequencing; Luciferase; Luciferin; Nucleotides; Pyrosequencing; Single stranded DNA binding protein

Indexed keywords

BIOASSAY; DNA SEQUENCES; ENZYMES; GENE ENCODING; NUCLEOTIDES;

APYRASE; ATP SULFURYLASE; DNA POLYMERASE; DNA SEQUENCING; LUCIFERASE; LUCIFERIN; PYROSEQUENCING; SINGLE-STRANDED DNA BINDING PROTEINS;

DNA;

SINGLE STRANDED DNA BINDING PROTEIN;

ARTICLE; DNA SEQUENCE; DNA SYNTHESIS; DNA TEMPLATE; PRIORITY JOURNAL;

EID: 0034669368     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2000.4808     Document Type: Article

References (21)

1. DNA sequencing with chain-terminating inhibitors
2. A novel method for nucleic acid sequence determination
3. Sequencing of megabase plus DNA by hybridization: Theory of the method
4. An oligonucleotide hybridization approach to DNA sequencing
5. A sequencing method based on real-time pyrophosphate
6. PCR-introduced loop structure as primer in DNA sequencing
7. Analyses of secondary structures in DNA by pyrosequencing
8. Direct solid-phase sequence analysis of the human p53 gene by use of multiplex polymerase chain reaction and alpha-thiotriphosphate nucleotides
9. Production, purification, and luminometric analysis of recombinant Saccharomyces cerevisiae MET3 adenosine triphosphate sulfurylase expressed in Escherichia coli
10. Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support
11. Escherichia coli single-strand binding protein organizes single-stranded DNA in nucleosome-like units
12. Enhancing PCR amplification and sequencing using DNA-binding proteins
13. DNA sequencing by primer walking with strings of contiguous hexamers
14. Automated DNA sequencing using 4-color fluorescent detection of reactions primed with hexamer strings
15. Single-stranded DNA binding proteins required for DNA replication
16. Determination of single-nucleotide polymorphisms by real-time pyrophosphate DNA sequencing
17. Single-nucleotide polymorphism analysis by pyrosequencing
18. Direct analysis of single-nucleotide polymorphism on double-stranded DNA by pyrosequencing
19. Kinetic studies and properties of potato apyrase
20. Kinetic mechanism of DNA polymerase I (Klenow fragment): Identification of a second conformational change and evaluation of the internal equilibrium constant