Analysis of the p53 tumor suppressor gene by pyrosequencing

BioTechniques

Volumn 28, Issue 1, 2000, Pages 140-147

  Ahmadian, A ^a   Lundeberg, J ^a   Nyren P ^a   Uhlen M ^a   Ronaghi, M ^a,b  

^a ROYAL INSTITUTE OF TECHNOLOGY   (Sweden)

^b STANFORD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN P53;

ALLELE; ARTICLE; CELL CYCLE; GENE AMPLIFICATION; GENE INACTIVATION; GENE MUTATION; GENE SEQUENCE; GENETIC VARIABILITY; HUMAN; HUMAN TISSUE; TUMOR SUPPRESSOR GENE;

EID: 0033988809     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/00281rr02     Document Type: Article

References (16)

1. Berg, C., A. Hedrum, A. Holmberg, F. Pontén, M. Uhlén and J. Lundeberg. 1995. Direct solid-phase sequence analysis of the human p53 gene by use of multiplex polymerase chain reaction and alpha-thiotriphosphate nucleotides. Clin. Chem. 41:1461-1466.
2. Fearon, E.R. and B. Vogelstein. 1990. A genetic model for colorectal tumorigenesis. Cell 61:759-767.
3. Harris, C.C. 1993. p53: at the crossroads of molecular carcinogenesis and risk assessment. Science 262:1980-1981.
4. Hedrum, A., F. Pontén, Z. Ren, J. Lundeberg, J. Pontén and M. Uhlén. 1994. Sequence-based analysis of the human p53 gene based on microdissection of tumor biopsy samples. BioTechniques 17:118-129.
5. Hollstein, M., D. Sidransky, B. Vogelstein and C.C. Harris. 1991. p53 mutations in human cancers. Science 253:49-53.
6. Hultman, T., S. Stahl, E. Homes and M. Uhlén. 1989. Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support. Nucleic Acids. Res. 17:4937-4946.
7. Karamohamed, S., J. Nilsson, K. Nourizad, M. Ronaghi, B. Pettersson and P. Nyrén. 1999. Production, purification, and luminometric analysis of recombinant saccharomyces cerevisiae MET3 adenosine triphosphate sulfurylase expressed in Escherichia coli. Protein Expr. Purif. 15:381-388.
8. Knudson, A.G., Jr. 1971. Mutation and cancer: statistical study of retinoblastoma. Proc. Natl. Acad. Sci. USA. 68:820-823.
9. Pontén, F., C. Berg, A. Ahmadian, M. Nister, J. Lundeberg, M. Uhlén and J. Pontén. 1997. Molecular pathology in basal cell cancer with p53 as a genetic marker. Oncogene 15:1059-1067.
10. Ren, Z.P., A. Ahmadian, F. Pont,n, M. Nister, C. Berg, J. Lundeberg, M. Uhlén and J. Pontén. 1997. Benign clonal keratinocyte patches with p53 mutations show no genetic link to synchronous squamous cell precancer or cancer in human skin. Am. J. Pathol. 150:1791-1803.
11. Ren, Z.P., A. Hedrum, F. Pontén, M. Nister, A. Ahmadian, J. Lundeberg, M. Uhlén and J. Pontén. 1996. Human epidermal cancer and accompanying precursors have identical p53 mutations different from p53 mutations in adjacent areas of clonally expanded non-neoplastic keratinocytes. Oncogene 12:765-773.
12. Ronaghi, M., M. Nygren, J. Lundeberg and P. Nyrén. 1999. Analyses of secondary structures in DNA by pyrosequencing. Anal. Biochem. 267:65-71.
13. Ronaghi, M., B. Pettersson, M. Uhlén and P. Nyrén. 1998. PCR-introduced loop structure as primer in DNA sequencing. BioTechniques 25:876-884.
14. Ronaghi, M., M. Uhlén and P. Nyrén. 1998. A sequencing method based on real-time pyrophosphate. Science 281:363, 365.
15. Satake, N., S. Urakami, Y. Hirayama, K. Izumi and O. Hino. 1998. Biallelic mutations of the Tsc2 gene in chemically induced rat renal cell carcinoma. Int. J. Cancer 77:895-900.
16. Westberg, J., A. Holmberg, M. Uhlén and B. Pettersson. 1999. Truncated fragments in polymerase chain reaction-based DNA sequencing. Electrophoresis 20:502-510.