Mutations to create thermostable reverse transcriptase with bacterial family A DNA polymerase from Thermotoga petrophila K4

Journal of Bioscience and Bioengineering

Volumn 113, Issue 3, 2012, Pages 315-321

  Sano, Sotaro ^a   Yamada, Yosuke ^a   Shinkawa, Tomoki ^a   Kato, Satoru ^a   Okada, Takashi ^a   Higashibata, Hiroki ^a   Fujiwara, Shinsuke ^a  

^a KWANSEI GAKUIN UNIVERSITY   (Japan)

Author keywords

DNA polymerase; PCR; Reverse transcriptase; RT PCR; Thermophilic bacteria; Thermostable enzyme

Indexed keywords

DNA POLYMERASE; PCR; REVERSE TRANSCRIPTASE; RT-PCR; THERMOPHILIC BACTERIA; THERMOSTABLE ENZYMES;

AMINO ACIDS; DNA; ENZYMES; POLYMERS; RNA;

POLYMERASE CHAIN REACTION;

ALANINE; BACTERIAL ENZYME; DNA POLYMERASE; FAMILY A DNA POLYMERASE; GLYCINE; HYDROXYL GROUP; LEUCINE; METHIONINE; MUTANT PROTEIN; PHENYLALANINE; PHOSPHODIESTERASE I; RNA DIRECTED DNA POLYMERASE; SPLEEN EXONUCLEASE; THREONINE; TYROSINE; UNCLASSIFIED DRUG;

ARTICLE; COMPUTER MODEL; DNA TEMPLATE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME BINDING; ENZYME CONFORMATION; GENE AMPLIFICATION; GENE MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN LOCALIZATION; THERMOSTABILITY; THERMOTOGA; THERMOTOGA PETROPHILA;

DNA PRIMERS; DNA-DIRECTED DNA POLYMERASE; EXONUCLEASES; GRAM-NEGATIVE ANAEROBIC BACTERIA; MOLECULAR SEQUENCE DATA; MUTATION; RECOMBINANT PROTEINS; RNA, RIBOSOMAL, 16S; RNA-DIRECTED DNA POLYMERASE;

BACTERIA (MICROORGANISMS); THERMOTOGA PETROPHILA;

EID: 84857687551     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2011.11.001     Document Type: Article

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