One-step RNA pathogen detection with reverse transcriptase activity of a mutated thermostable Thermus aquaticus DNA polymerase

Biotechnology Journal

Volumn 5, Issue 2, 2010, Pages 224-231

  Kranaster, Ramon ^a   Drum, Matthias ^a   Engel, Nicole ^a   Weidmann, Manfred ^b   Hufert, Frank T ^b   Marx, Andreas ^a  

^a UNIVERSITY OF KONSTANZ   (Germany)

^b UNIVERSITY MEDICAL CENTER GÖTTINGEN   (Germany)

Author keywords

DNA polymerase; G Quadruplex; RNA pathogen detection; RNA secondary structures; RT PCR

Indexed keywords

DNA POLYMERASE; G-QUADRUPLEX; G-QUADRUPLEXES; PATHOGEN DETECTION; RNA SECONDARY STRUCTURES;

CLONING; COMPUTER VIRUSES; DNA; DNA SEQUENCES; GENES; PLANTS (BOTANY); PROBES; RNA; VIRUSES;

PATHOGENS;

DNA POLYMERASE; GUANINE QUADRUPLEX; NUCLEASE; RNA; RNA DIRECTED DNA POLYMERASE; SHORT HAIRPIN RNA; TAQ POLYMERASE;

ANALYTICAL PARAMETERS; ARTICLE; BACTERIUM DETECTION; CONTROLLED STUDY; ENZYME ACTIVITY; GENE MUTATION; HIGH TEMPERATURE; MOLECULAR CLONING; NONHUMAN; PATHOGENESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; THERMOSTABILITY; THERMUS AQUATICUS; WILD TYPE;

HANTAVIRUS; MODELS, MOLECULAR; MUTATION; NUCLEIC ACID CONFORMATION; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; RNA-DIRECTED DNA POLYMERASE; TAQ POLYMERASE;

DOBRAVA-BELGRADE VIRUS; THERMUS AQUATICUS;

EID: 76649110196     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.200900200     Document Type: Article

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