Chimeric thermostable DNA polymerases with reverse transcriptase and attenuated 3′-5′ exonuclease activity

Biochemistry

Volumn 45, Issue 42, 2006, Pages 12786-12795

  Schönbrunner, Nancy J ^a   Fiss, Ellen H ^a   Budker, Olga ^a   Stoffel, Susanne ^a   Sigua, Christopher L ^a,b   Gelfand, David H ^a   Myers, Thomas W ^a  

^a ROCHE MOLECULAR SYSTEMS   (United States)

^b CELERA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; DNA; MIXTURES; MOLECULAR BIOLOGY; RNA; SYNTHESIS (CHEMICAL);

DNA POLYMERASE; EXONUCLEASE ACTIVITIES; MUTATIONS; REVERSE TRANSCRIPTASE;

ENZYME KINETICS;

COMPLEMENTARY DNA; DNA POLYMERASE; METAL ION; MUTANT PROTEIN; PHOSPHODIESTERASE I; RNA; RNA DIRECTED DNA POLYMERASE; SPLEEN EXONUCLEASE;

ARCHAEBACTERIUM; ARTICLE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME PURIFICATION; ENZYMOLOGY; MUTATION; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; THERMOSTABILITY; THERMOTOGA MARITIMA; THERMUS; WILD TYPE;

BACTERIAL PROTEINS; BASE SEQUENCE; DNA PRIMERS; DNA-DIRECTED DNA POLYMERASE; ENZYME STABILITY; EXONUCLEASES; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA-DIRECTED DNA POLYMERASE; SUBSTRATE SPECIFICITY; TEMPLATES, GENETIC; THERMODYNAMICS; THERMOTOGA MARITIMA; THERMUS;

HUMAN IMMUNODEFICIENCY VIRUS 1; THERMOTOGA MARITIMA; THERMUS;

EID: 33750373694     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi0609117     Document Type: Article

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