Expression in Escherichia coli of the catalytic domain of human proline oxidase

Protein Expression and Purification

Volumn 82, Issue 2, 2012, Pages 345-351

  Tallarita, Elena ^a,b   Pollegioni, Loredano ^a   Servi, Stefano ^a,b   Molla, Gianluca ^a  

^a UNIVERSITY OF INSUBRIA   (Italy)

^b POLITECNICO DI MILANO   (Italy)

Author keywords

Amino acid metabolism; Amino acid oxidase; l Proline; Neurotransmitter; Schizophrenia

Indexed keywords

HYBRID PROTEIN; PEPTIDE FRAGMENT; PROLINE DEHYDROGENASE;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; CHEMISTRY; ENZYME ACTIVE SITE; ESCHERICHIA COLI; GENE EXPRESSION; HUMAN; ISOLATION AND PURIFICATION; KINETICS; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; SOLUBILITY;

AMINO ACID SEQUENCE; BASE SEQUENCE; CATALYTIC DOMAIN; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; KINETICS; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; PROLINE OXIDASE; RECOMBINANT FUSION PROTEINS; SOLUBILITY;

ESCHERICHIA COLI;

EID: 84857469557     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2012.01.021     Document Type: Article

References (37)

1. J.A. Gogos, and D.J. Gerber Schizophrenia susceptibility genes: emergence of positional candidates and future directions Trends Pharmacol. Sci. 27 2006 226 233
2. A. Willis, H.U. Bender, G. Steel, and D. Valle PRODH variants and risk for schizophrenia Amino Acids 35 2008 673 679
3. H.J. Tuominen, J. Tiihonen, and K. Wahlbeck Glutamatergic drugs for schizophrenia: a systematic review and meta-analysis Schizophr. Res. 72 2005 225 234
4. K.C. Murphy Schizophrenia and velo-cardio-facial syndrome Lancet 359 2002 426 430
5. T.M. Maynard, D.W. Meechan, M.L. Dudevoir, D. Gopalakrishna, A.Z. Peters, C.C. Heindel, T.J. Sugimoto, Y. Wu, J.A. Lieberman, and A.S. LaMantia Mitochondrial localization and function of a subset of 22q11 deletion syndrome candidate genes Mol. Cell. Neurosci. 39 2008 439 451
6. J.J. Tanner Structural biology of proline catabolism Amino Acids 35 2008 719 730
7. J. Pandhare, S.P. Donald, S.K. Cooper, and J.M. Phang Regulation and function of proline oxidase under nutrient stress J. Cell. Biochem. 107 2009 759 768
8. C.A. Hu, D.B. Williams, S. Zhaorigetu, S. Khalil, G.H. Wan, and D. Valle Functional genomics and SNP analysis of human genes encoding proline metabolic enzymes Amino Acids 35 2008 655 664
9. Y.H. Lee, S. Nadaraia, D. Gu, D.F. Becker, and J.J. Tanner Structure of the proline dehydrogenase domain of the multifunctional PutA flavoprotein Nat. Struct. Mol. Biol. 10 2003 109 114
10. J. Phang, C.A. Hu, and D. Valle Disorders of proline and hydroxyproline metabolism C.R. Scriver, W.S. Sly, D. Valle, The Metabolic and Molecular Bases of Inherited Disease 2001 McGraw Hill, B.A. New York 1821 1838
11. H. Jacquet, G. Raux, F. Thibaut, B. Hecketsweiler, E. Houy, C. Demilly, S. Haouzir, G. Allio, G. Fouldrin, V. Drouin, J. Bou, M. Petit, D. Campion, and T. Frebourg PRODH mutations and hyperprolinemia in a subset of schizophrenic patients Hum. Mol. Genet. 11 2002 2243 2249
12. H. Liu, S.C. Heath, C. Sobin, J.L. Roos, B.L. Galke, M.L. Blundell, M. Lenane, B. Robertson, E.M. Wijsman, J.L. Rapoport, J.A. Gogos, and M. Karayiorgou Genetic variation at the 22q11 PRODH2/DGCR6 locus presents an unusual pattern and increases susceptibility to schizophrenia Proc. Natl. Acad. Sci. USA 99 2002 3717 3722
13. H.J. Williams, N. Williams, G. Spurlock, N. Norton, S. Zammit, G. Kirov, M.J. Owen, and M.C. O'Donovan Detailed analysis of PRODH and PsPRODH reveals no association with schizophrenia Am. J. Med. Gen. B - Neuropsychiatr. Genet. 120B 2003 42 46
14. H.U. Bender, S. Almashanu, G. Steel, C.A. Hu, W.W. Lin, A. Willis, A. Pulver, and D. Valle Functional consequences of PRODH missense mutations Am. J. Hum. Genet. 76 2005 409 420
15. Y. Liu, G.L. Borchert, S.P. Donald, B.A. Diwan, M. Anver, and J.M. Phang Proline oxidase functions as a mitochondrial tumor suppressor in human cancers Cancer Res. 69 2009 6414 6422
16. N. Krishnan, M.B. Dickman, and D.F. Becker Proline modulates the intracellular redox environment and protects mammalian cells against oxidative stress Free Radic. Biol. Med. 44 2008 671 681
17. S. Wanduragala, N. Sanyal, X.W. Liang, and D.F. Becker Purification and characterization of Put1p from Saccharomyces cerevisiae Arch. Biochem. Biophys. 498 2010 136 142
18. G. Molla, C. Vegezzi, M.S. Pilone, and L. Pollegioni Overexpression in Escherichia coli of a recombinant chimeric Rhodotorula gracilis D-amino acid oxidase Protein Expr. Purif. 14 1998 289 294
19. G. Molla, S. Sacchi, M. Bernasconi, M.S. Pilone, K. Fukui, and L. Pollegioni Characterization of human d-amino acid oxidase FEBS Lett. 580 2006 2358 2364
20. F. Volont, L. Pollegioni, G. Molla, L. Frattini, F. Marinelli, and L. Piubelli Production of recombinant cholesterol oxidase containing covalently bound FAD in Escherichia coli BMC Biotechnol. 10 2010 33
21. V. Job, G. Molla, M.S. Pilone, and L. Pollegioni Overexpression of a recombinant wild-type and His-tagged Bacillus subtilis glycine oxidase in Escherichia coli Eur. J. Biochem. 269 2002 1456 1463
22. V. Job, G.L. Marcone, M.S. Pilone, and L. Pollegioni Glycine oxidase from Bacillus subtilis. Characterization of a new flavoprotein J. Biol. Chem. 277 2002 6985 6993
23. N. Krishnan, and D.F. Becker Oxygen reactivity of PutA from Helicobacter species and proline-linked oxidative stress J. Bacteriol. 188 2006 1227 1235
24. M.G. Claros, and P. Vincens Computational method to predict mitochondrially imported proteins and their targeting sequences Eur. J. Biochem. 241 1996 779 786
25. T.A. White, N. Krishnan, D.F. Becker, and J.J. Tanner Structure and kinetics of monofunctional proline dehydrogenase from Thermus thermophilus J. Biol. Chem. 282 2007 14316 14327
26. A. Roy, A. Kucukural, and Y. Zhang I-TASSER: a unified platform for automated protein structure and function prediction Nat. Protoc. 5 2010 725 738
27. Z.R. Yang, R. Thomson, P. McNeil, and R.M. Esnouf RONN: the bio-basis function neural network technique applied to the detection of natively disordered regions in proteins Bioinformatics 21 2005 3369 3376
28. G. Molla, M. Bernasconi, S. Sacchi, M.S. Pilone, and L. Pollegioni Expression in Escherichia coli and in vitro refolding of the human protein pLG72 Protein Expr. Purif. 46 2006 150 155
29. C. Perez-Iratxeta, and M.A. Andrade-Navarro K2D2: estimation of protein secondary structure from circular dichroism spectra BMC Struct. Biol. 8 2008 25
30. V. Massey, F. Muller, R. Feldberg, M. Schuman, P.A. Sullivan, L.G. Howell, S.G. Mayhew, R.G. Matthews, and G.P. Foust Reactivity of flavoproteins with sulfite - possible relevance to problems of oxygen reactivity J. Biol. Chem. 244 1969 3999 4006
31. L. Pollegioni, S. Sacchi, L. Caldinelli, A. Boselli, M.S. Pilone, L. Piubelli, and G. Molla Engineering the properties of D-amino acid oxidases by a rational and a directed evolution approach Curr. Protein Pept. Sci. 8 2007 600 618
32. M.A. Moxley, J.J. Tanner, and D.F. Becker Steady-state kinetic mechanism of the proline:ubiquinone oxidoreductase activity of proline utilization A (PutA) from Escherichia coli Arch. Biochem. Biophys. 516 2011 113 120
33. R. Sherrington, J. Brynjolfsson, H. Petursson, M. Potter, K. Dudleston, B. Barraclough, J. Wasmuth, M. Dobbs, and H. Gurling Localization of a susceptibility locus for schizophrenia on chromosome-5 Nature 336 1988 164 167
34. T.R. Insel Rethinking schizophrenia Nature 468 2010 187 193
35. S. Sacchi, M. Bernasconi, M. Martineau, J.P. Mothet, M. Ruzzene, M.S. Pilone, L. Pollegioni, and G. Molla pLG72 modulates intracellular D-serine levels through its interaction with d-amino acid oxidase: effect on schizophrenia susceptibility J. Biol. Chem. 283 2008 22244 22256
36. J.A. Gogos, M. Santha, Z. Takacs, K.D. Beck, V. Luine, L.R. Lucas, J.V. Nadler, and M. Karayiorgou The gene encoding proline dehydrogenase modulates sensorimotor gating in mice Nat. Genet. 21 1999 434 439
37. J.D. Thompson, T.J. Gibson, F. Plewniak, F. Jeanmougin, and D.G. Higgins The CLUSTAL-X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools Nucleic Acids Res. 25 1997 4876 4882