In vivo genotoxicity study of titanium dioxide nanoparticles using comet assay following intratracheal instillation in rats

Regulatory Toxicology and Pharmacology

Volumn 62, Issue 1, 2012, Pages 1-6

  Naya, Masato ^a   Kobayashi, Norihiro ^a   Ema, Makoto ^a   Kasamoto, Sawako ^b   Fukumuro, Masahito ^b   Takami, Shigeaki ^b   Nakajima, Madoka ^b   Hayashi, Makoto ^b   Nakanishi, Junko ^a  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^b Biosafety Research Center Foods Drugs and Pesticides   (Japan)

Author keywords

Genotoxicity; In vivo; Nanomaterials; Titanium dioxide

Indexed keywords

DNA; MESYLIC ACID ETHYL ESTER; NANOPARTICLE; TITANIUM DIOXIDE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; COMET ASSAY; CONTROLLED STUDY; GENOTOXICITY; HISTOPATHOLOGY; IN VIVO STUDY; LUNG ALVEOLUS MACROPHAGE; MALE; NEUTROPHIL; NONHUMAN; PRIORITY JOURNAL; RAT;

ADMINISTRATION, INHALATION; ANIMALS; COMET ASSAY; MALE; NANOPARTICLES; PNEUMONIA; RATS; RATS, SPRAGUE-DAWLEY; TITANIUM; TOXICITY TESTS, ACUTE; TOXICITY TESTS, SUBACUTE;

RATTUS;

EID: 84856200028     PISSN: 02732300     EISSN: 10960295     Source Type: Journal    
DOI: 10.1016/j.yrtph.2011.12.002     Document Type: Article

References (38)

1. Bermudez E., Mangum J.B., Asgharian B., Wong B.A., Reverdy E.E., Janszen D.B., Hext P.M., Warheit D.B., Everitt J.I. Long-term pulmonary responses of three laboratory rodent species to subchronic inhalation of pigmentary titanium dioxide particles. Toxicol. Sci. 2002, 70:86-97.
2. 2 on bottle nose dolphin leukocytes. Anal. Bioanal. Chem. 2010, 396:619-623.
3. Bhattacharya K., Davoren M., Boertz J., Schins R.P.E., Hoffmann E., Dopp E. Titanium dioxide nanoparticles induce oxidative stress and DNA-adduct formation but not DNA-breakage in human lung cells. Part. Fibre Toxicol. 2009, 6:1-17.
4. Burlinson B., Tice R.R., Spite G., Agurell E., Brendler-Schwaab S.Y., Collins A.R., Escobar P., Honma M., Kumaravel T.S., Nakajima M., Sasaki Y.F., Thybaud V., Uno Y., Vasquez M., Hartmann A. Fourth International Workgroup on Genotoxicity testing: results of the in vivo Comet assay workgroup. Mutat. Res. 2007, 627:31-35.
5. Dunford R., Salinaro A., Cai L., Serpone N., Horikoshi S., Hidaka H., Knowland J. Chemical oxidation and DNA damage catalysed by inorganic sunscreen ingredients. FEBS Lett. 1997, 418:87-90.
6. 2. Hum. Exp. Toxicol. 2009, 28:339-352.
7. 2) nanoparticles at two trophic levels: plant and human lymphocytes. Chemosphere 2010, 81:1253-1262.
8. Gopalan R.C., Osman I.F., Amani A., de Matas M., Anderson D. The effect of zinc oxide and titanium dioxide nanoparticles in the comet assay with UVA photoactivation of human sperm and lymphocytes. Nanotoxicology 2009, 3:33-39.
9. Gurr J.-R., Wang A.S.S., Chen C.-H., Jan K.-Y. Ultrafine titanium dioxide particles in the absence of photoactivation can induce oxidative damage to human bronchial epithelial cells. Toxicology 2005, 213:66-73.
10. Hackenberg S., Friehs G., Froelich K., Ginzkey C., Koehler C., Scherzed A., Burghartz M., Hagen R., Kleinsasser N. Intracellular distribution, geno- and cytotoxic effects of nanosized titanium dioxide particles in the anatase crystal phase on human nasal mucosa cells. Toxicol. Lett. 2010, 195:9-14.
11. Hartman A., Agurell E., Beevers C., Brendler-Schwaab S.Y., Burlinson B., Collins A.R., Smith A., Spite G., Thybaud V., Tice R.R. Recommendations for conducting the in vivo alkaline Comet assay. Mutagenesis 2003, 18(1):45-51.
12. ICH Harmonised Tripartite Guideline 1998. http://www.ich.org/products/guidelines/safety/article/safety-guidelines.html.
13. Kang S.J., Kim B.M., Lee Y.J., Chung H.W. Titanium dioxide nanoparticles trigger P53-mediated damage response in peripheral blood lymphocytes. Environ. Mol. Mutagen. 2008, 49:399-405.
14. Karlsson H.L., Gustafsson J., Cronholm P., Moller L. Size-dependent toxicity of metal oxide particles - a comparison between nano- and micrometer size. Toxicol. Lett. 2009, 188:112-118.
15. 2 particles of different sizes and agglomerations in rats: different short- and long-term post-instillation results. Toxicology 2009, 264:110-118.
16. Landsiedel R., Ma-Hock L., Van Ravenzwaay B., Schulz M., Wiench K., Champ S., Schulte S., Wohllenben W., Oesch F. Gene toxicity studies on titanium dioxide and zinc oxide nanomaterials used for UV-protection in cosmetic formulations. Nanotoxicology 2010, 4:364-381.
17. Linnainmaa K., Kivipensas P., Vainio H. Toxicity and cytogenetic studies of ultrafine titanium dioxide in cultured rat liver epithelial cells. Toxicol. In Vitro 1997, 11:329-335.
18. Lu P.-L., Ho I.-C., Loe T.-C. Induction of sister chromatid exchanges and micronuclei by titanium dioxide in Chinese hamster ovary-Kl cells. Mutat. Res. 1998, 414:15-20.
19. Nakagawa Y., Wakuri S., Sakamoto K., Tanaka N. The photogenotoxicity of titanium dioxide particles. Mutat. Res. 1997, 394:125-132.
20. OECD-SIDS (2003) http://www.oecd.org/document/7/0,3746,en_2649_34379_1947463_1_1_1_1,00.html#Chapter_2.
21. Rahman O., Lohani M., Dopp E., Pemsel H., Jonas L., Weiss D.G., Schiffmann D. Evidence that ultrafine titanium dioxide induces micronuclei and apoptosis in Syrian hamster embryo fibroblasts. Environ. Health Perspect. 2002, 110:797-800.
22. 2) nanoparticle-induced cytotoxicity and oxidative DNA damage in fish cells. Mutat. Res. 2008, 640:113-122.
23. Shukla R.K., Sharma V., Pandey A.K., Singh S., Sultana S., Dhawan A. ROS-mediated genotoxicity induced by titanium dioxide nanoparticles in human epidermal cells. Toxicol. In Vitro 2011, 25:231-241.
24. Singh N., Manshian B., Jenkins G.L.S., Griffiths S.M., Williams P.M., Maffeis T.G.G., Wright C.J., Doak S.H. NanoGenotoxicology: the DNA damaging potential of engineered nanomaterials. Biomaterials 2009, 30:3891-3914.
25. Struwe M.S., Greulich K.-O., Suter W., Plappert-Helbig U. The photo comet assay - a fast screening assay for the determination of photogenotoxicity in vitro. Mutat. Res. 2007, 632:44-57.
26. Theogaraj E., Riley S., Hughes L., Maier M., Kirkland D. An investigation of the photo-clastogenic potential of ultrafine titanium dioxide particles. Mutat. Res. 2007, 634:205-219.
27. 2 particles differentially affect human skin fibroblasts exposed to UVA light. Free Radic. Biol. Med. 2010, 49:408-415.
28. Trouiller, B., Reliene, R., Westbrook, A., Solaimani, P., Schiestl, R.H., 2009. Titanium dioxide nanoparticles induce DNA damage and genetic instability in vivo in mice. Cancer Res. 69 (22), doi:10.1158/0008-5472.CAN-09-2496.
29. Turkez H. The role of ascorbic acid on titanium dioxide-induced genetic damage assessed by the comet assay and cytogenetic tests. Exp. Toxicol. Pathol. 2011, 63:453-457.
30. 2) nanoparticles on fish cells in vitro. Ecotoxicology 2008, 17:410-420.
31. 2 particles in cultured human lymphoblastoid cells. Mutat. Res. 2007, 628:99-106.
32. Warheit D.B., Hansen J.F., Yuen I.S., Kelly D.P., Snajdr S., Hartsky M.A. Inhalation of high concentrations of low toxicity dusts in rats results in pulmonary and macrophage clearance impairments. Toxicol. Appl. Pharmacol. 1997, 145:10-22.
33. 2 particle formulations: impact of surface treatments on particle toxicity. Toxicol. Sci. 2005, 88:514-524.
34. 2 Particles as a component of nanoparticle risk management. Toxicol. Lett. 2007, 171:99-110.
35. Warheit D.B., Webb T.R., Colvin V.L., Reed K.L., Sayes C.M. Pulmonary bioassay studies with nanoscale and fine quartz particles in rats: toxicity is not dependent upon particle size but on surface characteristics. Toxicol. Sci. 2007, 95:270-280.
36. 2 particles: differential responses related to surface properties. Toxicology 2007, 230:90-104.
37. Warheit D.B., Sayes C.M., Reed K.L., Swain K.A. Health effects related to nanoparticle exposures: environmental, health and safety considerations for assessing hazards and risks. Pharmacol. Ther. 2008, 120:35-42.
38. Xu A., Chai Y., Nohmi T., Hei T.K. Genotoxic response to titanium dioxide nanoparticles and fullerene in gpt delta transgenic MEF cells. Part. Fibre Toxicol. 2009, 6:3. 10.1186/1743-8977-63.