3D multicolor super-resolution imaging offers improved accuracy in neuron tracing

PLoS ONE

Volumn 7, Issue 1, 2012, Pages

  Lakadamyali, Melike ^a   Babcock, Hazen ^b   Bates, Mark ^a   Zhuang, Xiaowei ^b,c,d   Lichtman, Jeff ^a  

^a HARVARD UNIVERSITY   (United States)

^b HARVARD UNIVERSITY   (United States)

^c HARVARD UNIVERSITY   (United States)

^d HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE; PROTEIN ANTIBODY;

ANIMAL CELL; ARTICLE; BRAIN MAPPING; BRAIN NERVE CELL; CELL ACTIVITY; CELL DENSITY; CELL FUNCTION; CELL LABELING; CELL MEMBRANE POTENTIAL; CELLULAR DISTRIBUTION; CONFOCAL MICROSCOPY; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; EMBRYO; HIPPOCAMPUS; IMAGE ANALYSIS; INTERMETHOD COMPARISON; MULTICOLOR SUPER RESOLUTION IMAGING; NEUROANATOMICAL TRACT TRACING; NEUROIMAGING; NONHUMAN; PROTEIN TARGETING; RAT; STOCHASTIC OPTICAL RECONSTRUCTION MICROSCOPY; THREE DIMENSIONAL IMAGING; WILD TYPE; ANIMAL; CELL CULTURE; CELL TRACKING; COLOR; COMPARATIVE STUDY; CYTOLOGY; EVALUATION; FEMALE; IMAGE ENHANCEMENT; METHODOLOGY; NERVE CELL; NEWBORN; PREGNANCY; PRIMARY CELL CULTURE; SENSITIVITY AND SPECIFICITY; SPRAGUE DAWLEY RAT; ULTRASTRUCTURE;

RATTUS;

ANIMALS; ANIMALS, NEWBORN; CELL TRACKING; CELLS, CULTURED; COLOR; FEMALE; HIPPOCAMPUS; IMAGE ENHANCEMENT; IMAGING, THREE-DIMENSIONAL; MICROSCOPY, CONFOCAL; NEUROANATOMICAL TRACT-TRACING TECHNIQUES; NEURONS; PREGNANCY; PRIMARY CELL CULTURE; RATS; RATS, SPRAGUE-DAWLEY; SENSITIVITY AND SPECIFICITY;

EID: 84856194192     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0030826     Document Type: Article

References (28)

1. Lichtman JW, Livet J, Sanes JR, (2008) A technicolour approach to the connectome. Nat Rev Neurosci 9: 417-422.
2. Smith SJ, (2007) Circuit reconstruction tools today. Curr Opin Neurobiol 17: 601-608.
3. Helmstaedter M, Briggman KL, Denk W, (2008) 3D structural imaging of the brain with photons and electrons. Curr Opin Neurobiol 18: 633-641.
4. Coulson A, Sulston J, Brenner S, Karn J, (1986) Toward a physical map of the genome of the nematode Caenorhabditis elegans. Proc Natl Acad Sci U S A 83: 7821-7825.
5. Denk W, Horstmann H, (2004) Serial block-face scanning electron microscopy to reconstruct three-dimensional tissue nanostructure. PLoS Biol 2: e329.
6. Bock DD, Lee WC, Kerlin AM, Andermann ML, Hood G, et al. (2011) Network anatomy and in vivo physiology of visual cortical neurons. Nature 471: 177-182.
7. Briggman KL, Helmstaedter M, Denk W, (2011) Wiring specificity in the direction-selectivity circuit of the retina. Nature 471: 183-188.
8. Webster P, Schwarz H, Griffiths G, (2008) Preparation of cells and tissues for immuno EM. Methods Cell Biol 88: 45-58.
9. Lichtman JW, Conchello JA, (2005) Fluorescence microscopy. Nat Methods 2: 910-919.
10. Micheva KD, Busse B, Weiler NC, O'Rourke N, Smith SJ, (2010) Single-synapse analysis of a diverse synapse population: proteomic imaging methods and markers. Neuron 68: 639-653.
11. Micheva KD, Smith SJ, (2007) Array tomography: a new tool for imaging the molecular architecture and ultrastructure of neural circuits. Neuron 55: 25-36.
12. Livet J, Weissman TA, Kang H, Draft RW, Lu J, et al. (2007) Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. Nature 450: 56-62.
13. Mishchenko Y, (2010) On optical detection of densely labeled synapses in neuropil and mapping connectivity with combinatorially multiplexed fluorescent synaptic markers. PLoS One 5: e8853.
14. Huang B, Babcock H, Zhuang X, (2010) Breaking the diffraction barrier: super-resolution imaging of cells. Cell 143: 1047-1058.
15. Hell SW, (2007) Far-field optical nanoscopy. Science 316: 1153-1158.
16. Rust MJ, Bates M, Zhuang X, (2006) Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). Nat Methods 3: 793-795.
17. Betzig E, Patterson GH, Sougrat R, Lindwasser OW, Olenych S, et al. (2006) Imaging intracellular fluorescent proteins at nanometer resolution. Science 313: 1642-1645.
18. Hess ST, Girirajan TP, Mason MD, (2006) Ultra-high resolution imaging by fluorescence photoactivation localization microscopy. Biophys J 91: 4258-4272.
19. Huang B, Wang W, Bates M, Zhuang X, (2008) Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy. Science 319: 810-813.
20. Bates M, Huang B, Dempsey GT, Zhuang X, (2007) Multicolor super-resolution imaging with photo-switchable fluorescent probes. Science 317: 1749-1753.
21. Shroff H, Galbraith CG, Galbraith JA, Betzig E, (2008) Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics. Nat Methods 5: 417-423.
22. Jones SA, Shim SH, He J, Zhuang X, (2011) Fast, three-dimensional super-resolution imaging of live cells. Nat Methods 8: 499-508.
23. Huang B, Jones SA, Brandenburg B, Zhuang X, (2008) Whole-cell 3D STORM reveals interactions between cellular structures with nanometer-scale resolution. Nat Methods 5: 1047-1052.
24. Dempsey GT, Vaughan JC, Chen KH, Bates M, Zhuang X, (2011) Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging. Nat Methods.
25. Kay JN, Roeser T, Mumm JS, Godinho L, Mrejeru A, et al. (2004) Transient requirement for ganglion cells during assembly of retinal synaptic layers. Development 131: 1331-1342.
26. Dani A, Huang B, Bergan J, Dulac C, Zhuang X, (2010) Super-resolution imaging of chemical synapses in the brain. Neuron 68: 843-856.
27. Banker G, Goslin K, (1998) Culturing Nerve Cells. Cambridge The MIT Press.
28. Huang B, Bates M, Zhuang X, (2009) Super-resolution fluorescence microscopy. Annu Rev Biochem 78: 993-1016.