A protocol for differentiation of human intestinal Caco-2 cells in asymmetric serum-containing medium

Toxicology in Vitro

Volumn 26, Issue 8, 2012, Pages 1252-1255

  Ferruzza, Simonetta ^a   Rossi, Carlotta ^a   Scarino, Maria Laura ^a   Sambuy, Yula ^a  

^a ISTITUTO NAZIONALE DELLA NUTRIZIONE   (Italy)

Author keywords

3Rs; Alkaline phosphatase; Basolateral medium; Differentiation protocol; Enterocytes; Foetal bovine serum; Permeability; Serum reduction

Indexed keywords

ALKALINE PHOSPHATASE;

APICAL MEMBRANE; ARTICLE; BASOLATERAL MEMBRANE; CACO-2 CELL LINE; CELL CULTURE; CELL DIFFERENTIATION; CELL FUNCTION; CELL MEMBRANE PERMEABILITY; CELL STRUCTURE; CLINICAL PROTOCOL; COLON ADENOCARCINOMA; CONTROLLED STUDY; ENZYME ACTIVITY; HUMAN; HUMAN CELL; IN VITRO STUDY; INTESTINE CELL; SERUM CONTAINING MEDIUM; TIGHT JUNCTION; ANIMAL; BIOLOGICAL MODEL; CATTLE; CELL STRAIN CACO 2; COMPARATIVE STUDY; CULTURE MEDIUM; CULTURE TECHNIQUE; ENZYMOLOGY; FETUS BLOOD; METABOLISM; PERMEABILITY; PHYSIOLOGY;

ALKALINE PHOSPHATASE; ANIMALS; CACO-2 CELLS; CATTLE; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CULTURE MEDIA; ENTEROCYTES; FETAL BLOOD; HUMANS; MODELS, BIOLOGICAL; PERMEABILITY; TIGHT JUNCTIONS;

EID: 84856153560     PISSN: 08872333     EISSN: 18793177     Source Type: Journal    
DOI: 10.1016/j.tiv.2012.01.008     Document Type: Article

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