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Volumn 163, Issue 1, 2012, Pages 64-72

Reverse transcriptase real-time PCR for detection and quantification of viable Campylobacter jejuni directly from poultry faecal samples

Author keywords

Campylobacter jejuni; Campylobacter survival; Chicken faeces; MRNA; RT qPCR

Indexed keywords

DNA; RNA;

EID: 84855920658     PISSN: 09232508     EISSN: 17697123     Source Type: Journal    
DOI: 10.1016/j.resmic.2011.10.007     Document Type: Article
Times cited : (17)

References (41)
  • 3
    • 6944242331 scopus 로고    scopus 로고
    • Detection of seven virulence and toxin genes of Campylobacter jejuni and Campylobacter coli isolates from different sources and cytolethal distending toxin production suggest potential diversity of pathogenic properties among isolates
    • Bang D.D., Scheutz F., Gradel K.O., Nielsen E.M., Pedersen K., Engberg J., Gerner-Smidt P., Handberg K., Madsen M. Detection of seven virulence and toxin genes of Campylobacter jejuni and Campylobacter coli isolates from different sources and cytolethal distending toxin production suggest potential diversity of pathogenic properties among isolates. Genome Lett. 2003, 2:62-72.
    • (2003) Genome Lett. , vol.2 , pp. 62-72
    • Bang, D.D.1    Scheutz, F.2    Gradel, K.O.3    Nielsen, E.M.4    Pedersen, K.5    Engberg, J.6    Gerner-Smidt, P.7    Handberg, K.8    Madsen, M.9
  • 4
    • 0026071369 scopus 로고
    • Detection of viable Legionella pneumophila in water by polymerase chain reaction and gene probe methods
    • Bej A.K., Mahbubani M.H., Atlas R.M. Detection of viable Legionella pneumophila in water by polymerase chain reaction and gene probe methods. Appl. Environ. Microbiol. 1991, 57:597-600.
    • (1991) Appl. Environ. Microbiol. , vol.57 , pp. 597-600
    • Bej, A.K.1    Mahbubani, M.H.2    Atlas, R.M.3
  • 5
    • 0031938501 scopus 로고    scopus 로고
    • Extended survival and persistence of Campylobacter spp. in water and aquatic biofilms and their detection by immunofluorescent-antibody and -rRNA staining
    • Buswell C.M., Herlihy Y.M., Lawrence L.M., McGuiggan J.T.M., Marsh P.D., Keevil C.W., Leach S.A. Extended survival and persistence of Campylobacter spp. in water and aquatic biofilms and their detection by immunofluorescent-antibody and -rRNA staining. Appl. Environ. Microbiol. 1998, 64:733-741.
    • (1998) Appl. Environ. Microbiol. , vol.64 , pp. 733-741
    • Buswell, C.M.1    Herlihy, Y.M.2    Lawrence, L.M.3    McGuiggan, J.T.M.4    Marsh, P.D.5    Keevil, C.W.6    Leach, S.A.7
  • 6
    • 34248212653 scopus 로고    scopus 로고
    • Detection of Campylobacter jejuni and Campylobacter coli in chicken meat samples by real-time nucleic acid sequence-based amplification with molecular beacons
    • Churruca E., Girbau C., Martínez I., Mateo E., Alonso R., Fernández-Astorga A. Detection of Campylobacter jejuni and Campylobacter coli in chicken meat samples by real-time nucleic acid sequence-based amplification with molecular beacons. Int. J. Food Microbiol. 2007, 117:85-90.
    • (2007) Int. J. Food Microbiol. , vol.117 , pp. 85-90
    • Churruca, E.1    Girbau, C.2    Martínez, I.3    Mateo, E.4    Alonso, R.5    Fernández-Astorga, A.6
  • 8
    • 16444362516 scopus 로고    scopus 로고
    • MRNA detection by reverse transcription-PCR for monitoring viability and potential virulence in a pathogenic strain of Vibrio parahaemolyticus in viable but nonculturable state
    • Coutard F., Pommepuy M., Loaec S., Hervio-Heath D. mRNA detection by reverse transcription-PCR for monitoring viability and potential virulence in a pathogenic strain of Vibrio parahaemolyticus in viable but nonculturable state. J. Appl. Microbiol. 2005, 98:951-961.
    • (2005) J. Appl. Microbiol. , vol.98 , pp. 951-961
    • Coutard, F.1    Pommepuy, M.2    Loaec, S.3    Hervio-Heath, D.4
  • 9
    • 0037396433 scopus 로고    scopus 로고
    • Prevalence of 11 pathogenic genes of Campylobacter jejuni by PCR in strains isolated from humans, poultry meat and broiler and bovine faeces
    • Datta S., Niwa H., Itoh K. Prevalence of 11 pathogenic genes of Campylobacter jejuni by PCR in strains isolated from humans, poultry meat and broiler and bovine faeces. J. Med. Microbiol. 2003, 52:345-348.
    • (2003) J. Med. Microbiol. , vol.52 , pp. 345-348
    • Datta, S.1    Niwa, H.2    Itoh, K.3
  • 11
    • 34249908634 scopus 로고    scopus 로고
    • Insufficient differentiation of live and dead Campylobacter jejuni and Listeria monocytogenes cells by ethidium monoazide (EMA) compromises EMA/real-time PCR
    • Flekna G., Stefanic P., Wagner M., Smulders F.J.M., Mozina S.S., Hein I. Insufficient differentiation of live and dead Campylobacter jejuni and Listeria monocytogenes cells by ethidium monoazide (EMA) compromises EMA/real-time PCR. Res. Microbiol. 2007, 158:405-412.
    • (2007) Res. Microbiol. , vol.158 , pp. 405-412
    • Flekna, G.1    Stefanic, P.2    Wagner, M.3    Smulders, F.J.M.4    Mozina, S.S.5    Hein, I.6
  • 15
    • 3242881429 scopus 로고    scopus 로고
    • Direct quantification of Campylobacter jejuni and Campylobacter lanienae in feces of cattle by real-time quantitative PCR
    • Inglis G.D., Kalischuk L.D. Direct quantification of Campylobacter jejuni and Campylobacter lanienae in feces of cattle by real-time quantitative PCR. Appl. Environ. Microbiol. 2004, 70:2296-2306.
    • (2004) Appl. Environ. Microbiol. , vol.70 , pp. 2296-2306
    • Inglis, G.D.1    Kalischuk, L.D.2
  • 16
    • 0035172404 scopus 로고    scopus 로고
    • Macrolide resistance in Campylobacter coli of animal origin in Denmark
    • Jensen L.B., Aarestrup F.M. Macrolide resistance in Campylobacter coli of animal origin in Denmark. Antimicrob. Agents Chemother. 2001, 45:371-372.
    • (2001) Antimicrob. Agents Chemother. , vol.45 , pp. 371-372
    • Jensen, L.B.1    Aarestrup, F.M.2
  • 17
    • 77955580586 scopus 로고    scopus 로고
    • Rapid quantification of viable Campylobacter bacteria on chicken carcasses, using real-time PCR and propidium monoazide treatment, as a tool for quantitative risk assessment
    • Josefsen M.H., Lofstrom C., Hansen T.B., Christensen L.S., Olsen J.E., Hoorfar J. Rapid quantification of viable Campylobacter bacteria on chicken carcasses, using real-time PCR and propidium monoazide treatment, as a tool for quantitative risk assessment. Appl. Environ. Microbiol. 2010, 76:5097-5104.
    • (2010) Appl. Environ. Microbiol. , vol.76 , pp. 5097-5104
    • Josefsen, M.H.1    Lofstrom, C.2    Hansen, T.B.3    Christensen, L.S.4    Olsen, J.E.5    Hoorfar, J.6
  • 18
    • 0027363920 scopus 로고
    • Polymerase chain reaction detection of nonviable bacterial pathogens
    • Josephson K.L., Gerba C.P., Pepper I.L. Polymerase chain reaction detection of nonviable bacterial pathogens. Appl. Environ. Microbiol. 1993, 59:3513-3515.
    • (1993) Appl. Environ. Microbiol. , vol.59 , pp. 3513-3515
    • Josephson, K.L.1    Gerba, C.P.2    Pepper, I.L.3
  • 19
    • 0031873016 scopus 로고    scopus 로고
    • Characterisation of the thermal stress response of Campylobacter jejuni
    • Konkel M.E., Kim B.J., Klena J.D., Young C.R., Ziprin R. Characterisation of the thermal stress response of Campylobacter jejuni. Infect. Immun. 1998, 66:3666-3672.
    • (1998) Infect. Immun. , vol.66 , pp. 3666-3672
    • Konkel, M.E.1    Kim, B.J.2    Klena, J.D.3    Young, C.R.4    Ziprin, R.5
  • 20
    • 77955959302 scopus 로고    scopus 로고
    • Detection of human intestinal catalase-negative, gram-positive cocci by rRNA-targeted reverse transcription-PCR
    • Kubota H., Tsuji H., Matsuda K., Kurakawa T., Asahara T., Nomoto K. Detection of human intestinal catalase-negative, gram-positive cocci by rRNA-targeted reverse transcription-PCR. Appl. Environ. Microbiol. 2010, 76:5440-5451.
    • (2010) Appl. Environ. Microbiol. , vol.76 , pp. 5440-5451
    • Kubota, H.1    Tsuji, H.2    Matsuda, K.3    Kurakawa, T.4    Asahara, T.5    Nomoto, K.6
  • 21
    • 46749145029 scopus 로고    scopus 로고
    • Cytokine responses in primary chicken embryo intestinal cells infected with Campylobacter jejuni strains of human and chicken origin and the expression of bacterial virulence-associated genes
    • Li Y.-P., Ingmer H., Madsen M., Bang D. Cytokine responses in primary chicken embryo intestinal cells infected with Campylobacter jejuni strains of human and chicken origin and the expression of bacterial virulence-associated genes. BMC Microbiol. 2008, 8:107.
    • (2008) BMC Microbiol. , vol.8 , pp. 107
    • Li, Y.-P.1    Ingmer, H.2    Madsen, M.3    Bang, D.4
  • 22
    • 77649205641 scopus 로고    scopus 로고
    • Preliminary development and validation of a real-time reverse transcription PCR assay for the semi-quantification of viable Campylobacter jejuni in water samples
    • Lin S., Gilpin B., Scholes P., Podivinsky E., Klena J., Savill M. Preliminary development and validation of a real-time reverse transcription PCR assay for the semi-quantification of viable Campylobacter jejuni in water samples. Water Sci. Technol. 2009, 60:3151-3158.
    • (2009) Water Sci. Technol. , vol.60 , pp. 3151-3158
    • Lin, S.1    Gilpin, B.2    Scholes, P.3    Podivinsky, E.4    Klena, J.5    Savill, M.6
  • 23
    • 77950426109 scopus 로고    scopus 로고
    • Quantification of viable but nonculturable Escherichia coli O157:H7 by targeting the rpoS mRNA
    • Liu Y., Wang C., Fung C., Li X.-F. Quantification of viable but nonculturable Escherichia coli O157:H7 by targeting the rpoS mRNA. Anal. Chem. 2010, 82:2612-2615.
    • (2010) Anal. Chem. , vol.82 , pp. 2612-2615
    • Liu, Y.1    Wang, C.2    Fung, C.3    Li, X.-F.4
  • 24
    • 8644265908 scopus 로고    scopus 로고
    • Detection of Campylobacter spp. in chicken fecal samples by real-time PCR
    • Lund M., Nordentoft S., Pedersen K., Madsen M. Detection of Campylobacter spp. in chicken fecal samples by real-time PCR. J. Clin. Microbiol. 2004, 42:5125-5132.
    • (2004) J. Clin. Microbiol. , vol.42 , pp. 5125-5132
    • Lund, M.1    Nordentoft, S.2    Pedersen, K.3    Madsen, M.4
  • 26
    • 36749036945 scopus 로고    scopus 로고
    • The mythology of PCR: a warning to the wise
    • Springer, US, J. Maurer (Ed.)
    • Maurer J.J. The mythology of PCR: a warning to the wise. PCR Methods in Foods 2006, 27-40. Springer, US. J. Maurer (Ed.).
    • (2006) PCR Methods in Foods , pp. 27-40
    • Maurer, J.J.1
  • 27
    • 0004883372 scopus 로고    scopus 로고
    • Distribution of serotypes of Campylobacter jejuni and C. coli from Danish patients, poultry, cattle and swine
    • Møller Nielsen E., Engberg J., Madsen M. Distribution of serotypes of Campylobacter jejuni and C. coli from Danish patients, poultry, cattle and swine. FEMS Immunol. Med. Microbiol. 1997, 19:47-56.
    • (1997) FEMS Immunol. Med. Microbiol. , vol.19 , pp. 47-56
    • Møller Nielsen, E.1    Engberg, J.2    Madsen, M.3
  • 29
    • 42349094631 scopus 로고    scopus 로고
    • Real-time PCR approach for detection of environmental sources of Campylobacter strains colonizing broiler flocks
    • Ridley A.M., Allen V.M., Sharma M., Harris J.A., Newell D.G. real-time PCR approach for detection of environmental sources of Campylobacter strains colonizing broiler flocks. Appl. Environ. Microbiol. 2008, 74:2492-2504.
    • (2008) Appl. Environ. Microbiol. , vol.74 , pp. 2492-2504
    • Ridley, A.M.1    Allen, V.M.2    Sharma, M.3    Harris, J.A.4    Newell, D.G.5
  • 30
    • 0036128379 scopus 로고    scopus 로고
    • Messenger RNA-based RT-PCR detection of viable Salmonella
    • Rijpens N., Jannes G., Herman L. Messenger RNA-based RT-PCR detection of viable Salmonella. Int. Dairy J. 2002, 12:233-238.
    • (2002) Int. Dairy J. , vol.12 , pp. 233-238
    • Rijpens, N.1    Jannes, G.2    Herman, L.3
  • 31
    • 77956926265 scopus 로고    scopus 로고
    • An evaluation of survival and detection of Campylobacter jejuni and C. coli in broiler caecal contents using culture-based methods
    • Rodgers J.D., Clifton-Hadley F.A., Marin C., Vidal A.B. An evaluation of survival and detection of Campylobacter jejuni and C. coli in broiler caecal contents using culture-based methods. J. Appl. Microbiol. 2010, 109:1244-1252.
    • (2010) J. Appl. Microbiol. , vol.109 , pp. 1244-1252
    • Rodgers, J.D.1    Clifton-Hadley, F.A.2    Marin, C.3    Vidal, A.B.4
  • 32
    • 34250369243 scopus 로고    scopus 로고
    • Quantitative detection of Campylobacter jejuni on fresh chicken carcasses by real-time PCR
    • Rönner A.-C., Lindmark H. Quantitative detection of Campylobacter jejuni on fresh chicken carcasses by real-time PCR. J. Food Prot. 2007, 70:1373-1378.
    • (2007) J. Food Prot. , vol.70 , pp. 1373-1378
    • Rönner, A.-C.1    Lindmark, H.2
  • 33
    • 2342597065 scopus 로고    scopus 로고
    • Direct real-time PCR quantification of Campylobacter jejuni in chicken fecal and cecal samples by integrated cell concentration and DNA purification
    • Rudi K., Hoidal H.K., Katla T., Johansen B.K., Nordal J., Jakobsen K.S. Direct real-time PCR quantification of Campylobacter jejuni in chicken fecal and cecal samples by integrated cell concentration and DNA purification. Appl. Environ. Microbiol. 2004, 70:790-797.
    • (2004) Appl. Environ. Microbiol. , vol.70 , pp. 790-797
    • Rudi, K.1    Hoidal, H.K.2    Katla, T.3    Johansen, B.K.4    Nordal, J.5    Jakobsen, K.S.6
  • 34
    • 13544254304 scopus 로고    scopus 로고
    • Use of ethidium monoazide and PCR in combination for quantification of viable and dead cells in complex samples
    • Rudi K., Moen B., Dromtorp S.M., Holck A.L. Use of ethidium monoazide and PCR in combination for quantification of viable and dead cells in complex samples. Appl. Environ. Microbiol. 2005, 71:1018-1024.
    • (2005) Appl. Environ. Microbiol. , vol.71 , pp. 1018-1024
    • Rudi, K.1    Moen, B.2    Dromtorp, S.M.3    Holck, A.L.4
  • 35
    • 0031957717 scopus 로고    scopus 로고
    • Detection of mRNA by reverse transcription-PCR as an indicator of viability in Escherichia coli cells
    • Sheridan G.E.C., Masters C.I., Shallcross J.A., Mackey B.M. Detection of mRNA by reverse transcription-PCR as an indicator of viability in Escherichia coli cells. Appl. Environ. Microbiol. 1998, 64:1313-1318.
    • (1998) Appl. Environ. Microbiol. , vol.64 , pp. 1313-1318
    • Sheridan, G.E.C.1    Masters, C.I.2    Shallcross, J.A.3    Mackey, B.M.4
  • 36
    • 0032950736 scopus 로고    scopus 로고
    • Evaluation of sampling methods for the detection of Salmonella in broiler flocks
    • Skov M.N., Carstensen B., Tornøe N., Madsen M. Evaluation of sampling methods for the detection of Salmonella in broiler flocks. J. Appl. Microbiol. 1999, 86:695-700.
    • (1999) J. Appl. Microbiol. , vol.86 , pp. 695-700
    • Skov, M.N.1    Carstensen, B.2    Tornøe, N.3    Madsen, M.4
  • 37
    • 0026594004 scopus 로고
    • Genome maps of Campylobacter jejuni and Campylobacter coli
    • Taylor D.E., Eaton M., Yan W., Chang N. Genome maps of Campylobacter jejuni and Campylobacter coli. J. Bacteriol. 1992, 174:2332-2337.
    • (1992) J. Bacteriol. , vol.174 , pp. 2332-2337
    • Taylor, D.E.1    Eaton, M.2    Yan, W.3    Chang, N.4
  • 38
    • 77949572522 scopus 로고    scopus 로고
    • Real-time reverse transcriptase PCR for the rapid and sensitive detection of Salmonella typhimurium from pork
    • Techathuvanan C., Draughon F.A., D'Souza D.H. Real-time reverse transcriptase PCR for the rapid and sensitive detection of Salmonella typhimurium from pork. J. Food Prot. 2010, 73:507-514.
    • (2010) J. Food Prot. , vol.73 , pp. 507-514
    • Techathuvanan, C.1    Draughon, F.A.2    D'Souza, D.H.3
  • 40
    • 12144272204 scopus 로고    scopus 로고
    • Risk assessment of false-positive quantitative real-time PCR results in food, due to detection of DNA originating from dead cells
    • Wolffs P., Norling B., Rådström P. Risk assessment of false-positive quantitative real-time PCR results in food, due to detection of DNA originating from dead cells. J. Microbiol. Methods 2005, 60:315-323.
    • (2005) J. Microbiol. Methods , vol.60 , pp. 315-323
    • Wolffs, P.1    Norling, B.2    Rådström, P.3


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