An evaluation of survival and detection of Campylobacter jejuni and C. coli in broiler caecal contents using culture-based methods

Journal of Applied Microbiology

Volumn 109, Issue 4, 2010, Pages 1244-1252

  Rodgers, J D ^a   Clifton Hadley, F A ^a   Marin, C ^b   Vidal, A B ^a  

^a VETERINARY LABORATORIES AGENCY   (United Kingdom)

^b UNIVERSIDAD CEU CARDENAL HERRERA   (Spain)

Author keywords

broiler; caeca; Campylobacter coli; Campylobacter jejuni; detection; enrichment; enumeration

Indexed keywords

ALGAE; POLYSACCHARIDES;

BROILER; CAECUM; CAMPYLOBACTER COLI; CAMPYLOBACTER JEJUNI; CAMPYLOBACTERS; DETECTION; ENRICHMENT; ENUMERATION; PRESTON; THERMOPHILIC CAMPYLOBACTERS;

BACTERIA;

AGAR; PHOSPHATE BUFFERED SALINE;

AGAR; EUROPEAN UNION; SURVIVAL; THERMOPHILIC BACTERIUM;

ARTICLE; BACTERIAL SURVIVAL; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM ISOLATION; BROILER; CAMPYLOBACTER COLI; CAMPYLOBACTER JEJUNI; NONHUMAN; STORAGE TEMPERATURE; THERMOPHILIC BACTERIUM;

CAMPYLOBACTER; CAMPYLOBACTER COLI; CAMPYLOBACTER JEJUNI;

EID: 77956926265     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2010.04748.x     Document Type: Article

References (42)

1. Anon. (2007) Commission Decision 2007516EC of 19 July 2007 concerning a financial contribution from the Community towards a survey on the prevalence and antimicrobial resistance of Campylobacter spp. in broiler flocks and on the prevalence of Campylobacter spp. and Salmonella spp. in broiler carcasses to be carried out in the Member States. Available at the European Commission website: http://eurlex.europa.eu/LexUriServ/LexUriServ.do?uri=CELEX:32007D0516:EN:NOT.
2. Anon. (2009) The Community summary report on trends and sources of zoonoses and zoonotic agents in the European Union in 2007. EFSA J (2009), 223. Available at the EFSA website: http://www.efsa.europa.eu/EFSA/efsa-locale- 1178620753812-1211902269834.htm.
3. Arsenault, J., Letellier, A., Quessy, S., Normand, V. Boulianne, M. (2007) Prevalence and risk factors for Salmonella spp. and Campylobacter spp. caecal colonization in broiler chicken and turkey flocks slaughtered in Quebec, Canada. Prev Vet Med 81, 250 264.
4. Barrios, P.R., Reiersen, J., Lowman, R., Bisaillon, J.R., Michel, P., Fridriksdottir, V., Gunnarsson, E., Stern, N. et al. (2006) Risk factors for Campylobacter spp. colonization in broiler flocks in Iceland. Prev Vet Med 74, 264 278.
5. Berndtson, E., Emmanuelson, U., Engvall, A. Daneielsson-Tham, M.L. (1996) A 1-year epidemiological study of campylobacters in 18 Swedish Farms. Prev Vet Med 26, 167 185.
6. Best, E.L., Powell, E.J., Swift, C., Grant, K.A. Frost, J.A. (2003) Applicability of a rapid duplex real-time PCR assay for speciation of Campylobacter jejuni and Campylobacter coli directly from culture plates. FEMS Microbiol Lett 229, 237 241.
7. Blaser, M.J., Hardesty, H.L., Powers, B. Wang, W.L. (1980) Survival of Campylobacter fetus subsp. jejuni in biological milieus. J Clin Microbiol 11, 309 313.
8. Borck, B., Stryhn, H., Ersboll, A.K. Pedersen, K. (2002) Thermophilic Campylobacter spp. in turkey samples: evaluation of two automated enzyme immunoassays and conventional microbiological techniques. J Appl Microbiol 92, 574 582.
9. Chan, K.F., Le Tran, H., Kanenaka, R.Y. Kathariou, S. (2001) Survival of clinical and poultry-derived isolates of Campylobacter jejuni at a low temperature (4 degrees C). Appl Environ Microbiol 67, 4186 4191.
10. Corry, J.E.L., Post, D.E., Colin, P. Laisney, M.J. (1995) Culture media for the isolation of campylobacters. Int J Food Microbiol 26, 43 76.
11. Denis, M., Refregier-Petton, J., Laisney, M.J., Ermel, G. Salvat, G. (2001) Campylobacter contamination in French chicken production from farm to consumers. Use of a PCR assay for detection and identification of Campylobacter jejuni and Camp. coli. J Appl Microbiol 91, 255 267.
12. Evans, S.J. Sayers, A.R. (2000) A longitudinal study of campylobacter infection of broiler flocks in Great Britain. Prev Vet Med 46, 209 223.
13. Feldsine, P., Abeyta, C. Andrews, W.H. (2002) AOAC International methods committee guidelines for validation of qualitative and quantitative food microbiological official methods of analysis. J AOAC Int 85, 1187 1200.
14. Fitzgerald, C., Whichard, J. Nachamkin, I. (2008) Diagnosis and antimicrobial susceptibility of Campylobacter species. In Campylobacter, 3rd edn ed. Nachamkin, I., Szymanski, C.M. Blaser, M.J. pp. 227 243.
15. Georgsson, F., Thornorkelsson, A.E., Geirsdottir, M., Reiersen, J. Stern, N.J. (2006) The influence of freezing and duration of storage on Campylobacter and indicator bacteria in broiler carcasses. Food Microbiol 23, 677 683.
16. Gilpin, B.J., Robson, B., Scholes, P., Nourozi, F. Sinton, L.W. (2009) Survival of Campylobacter spp. in bovine faeces on pasture. Lett Appl Microbiol 48, 162 166.
17. Gun-Munro, J., Rennie, R.P., Thornley, J.H., Richardson, H.L., Hodge, D. Lynch, J. (1987) Laboratory and clinical evaluation of isolation media for Campylobacter jejuni. J Clin Microbiol 25, 2274 2277.
18. Habib, I., Sampers, I., Uyttendaele, M., Berkvens, D. De Zutter, L. (2008) Baseline data from a Belgium-wide survey of Campylobacter species contamination in chicken meat preparations and considerations for a reliable monitoring program. Appl Environ Microbiol 74, 5483 5489.
19. Hald, B., Wedderkopp, A. Madsen, M. (2000) Thermophilic Campylobacter spp. in Danish broiler production: a cross-sectional survey and a retrospective analysis of risk factors for occurrence in broiler flocks. Avian Pathol 29, 123 131.
20. Hudock, J.F., Borger, A.C. Kaspar, C.W. (2005) Temperature-dependent genome degradation in the coccoid form of Campylobacter jejuni. Curr Microbiol 50, 110 113.
21. Humphrey, T.J. (1989) An appraisal of the efficacy of pre-enrichment for the isolation of Campylobacter jejuni from water and food. J Appl Bacteriol 66, 119 126.
22. Humphrey, T., Mason, M. Martin, K. (1995) The isolation of Campylobacter jejuni from contaminated surfaces and its survival in diluents. Int J Food Microbiol 26, 295 303.
23. Karenlampi, R. Hanninen, M.L. (2004) Survival of Campylobacter jejuni on various fresh produce. Int J Food Microbiol 97, 187 195.
24. Lawson, A.J., Shafi, M.S., Pathak, K. Stanley, J. (1998) Detection of campylobacter in gastroenteritis: comparison of direct PCR assay of faecal samples with selective culture. Epidemiol Infect 121, 547 553.
25. Luechtefeld, N.W., Wang, W.L., Blaser, M.J. Reller, L.B. (1981) Evaluation of transport and storage techniques for isolation of Campylobacter fetus subsp. jejuni from turkey cecal specimens. J Clin Microbiol 13, 438 443.
26. Lund, M., Wedderkopp, A., Waino, M., Nordentoft, S., Bang, D.D., Pedersen, K. Madsen, M. (2003) Evaluation of PCR for detection of Campylobacter in a national broiler surveillance programme in Denmark. J Appl Microbiol 94, 929 935.
27. Lund, M., Nordentoft, S., Pedersen, K. Madsen, M. (2004) Detection of Campylobacter spp. in chicken fecal samples by real-time PCR. J Clin Microbiol 42, 5125 5132.
28. Maher, M., Finnegan, C., Collins, E., Ward, B., Carroll, C. Cormican, M. (2003) Evaluation of culture methods and a DNA probe-based PCR assay for detection of Campylobacter species in clinical specimens of feces. J Clin Microbiol 41, 2980 2986.
29. McDowell, S.W., Menzies, F.D., McBride, S.H., Oza, A.N., McKenna, J.P., Gordon, A.W. Neill, S.D. (2008) Campylobacter spp. in conventional broiler flocks in Northern Ireland: epidemiology and risk factors. Prev Vet Med 84, 261 276.
30. Merino, F.J., Agulla, A., Villasante, P.A., Diaz, A., Saz, J.V. Velasco, A.C. (1986) Comparative efficacy of seven selective media for isolating Campylobacter jejuni. J Clin Microbiol 24, 451 452.
31. Morales-Rayas, R., Wolffs, P.F. Griffiths, M.W. (2008) Immunocapture and real-time PCR to detect Campylobacter spp. J Food Prot 71, 2543 2547.
32. Musgrove, M.T., Berrang, M.E., Byrd, J.A., Stern, N.J. Cox, N.A. (2001) Detection of Campylobacter spp. in ceca and crops with and without enrichment. Poult Sci 80, 825 828.
33. Newell, D.G., Shreeve, J.E., Toszeghy, M., Domingue, G., Bull, S., Humphrey, T. Mead, G. (2001) Changes in the carriage of Campylobacter strains by poultry carcasses during processing in abattoirs. Appl Environ Microbiol 67, 2636 2640.
34. Ng, L.K., Taylor, D.E. Stiles, M.E. (1988) Characterization of freshly isolated Campylobacter coli strains and suitability of selective media for their growth. J Clin Microbiol 26, 518 523.
35. Oyarzabal, O.A., Macklin, K.S., Barbaree, J.M. Miller, R.S. (2005) Evaluation of agar plates for direct enumeration of Campylobacter spp. from poultry carcass rinses. Appl Environ Microbiol 71, 3351 3354.
36. Rosenquist, H., Sommer, H.M., Nielsen, N.L. Christensen, B.B. (2006) The effect of slaughter operations on the contamination of chicken carcasses with thermotolerant Campylobacter. Int J Food Microbiol 108, 226 232.
37. Russa, A.D., Bouma, A., Vernooij, J.C., Jacobs-Reitsma, W. Stegeman, J.A. (2005) No association between partial depopulation and Campylobacter spp. colonization of Dutch broiler flocks. Lett Appl Microbiol 41, 280 285.
38. Stern, N.J., Bannov, V.A., Svetoch, E.A., Mitsevich, E.V., Mitsevich, I.P., Volozhantsev, N.V., Gusev, V.V. Perelygin, V.V. (2004) Distribution and characterization of Campylobacter spp. from Russian poultry. J Food Prot 67, 239 245.
39. Van Overbeke, I., Duchateau, L., De Zutter, L., Albers, G. Ducatelle, R. (2006) A comparison survey of organic and conventional broiler chickens for infectious agents affecting health and food safety. Avian Dis 50, 196 200.
40. Wagenaar, J.A., Jacobs-Reitsma, W., Hofshagen, M. Newell, D. (2008) Poultry colonization with Campylobacter and its control at the primary production level. In Campylobacter, 3rd edn ed Nachamkin, I., Szymanski, C.M. Blaser, M.J. pp. 667 678.
41. Williams, L.K., McMeechan, A., Baalham, T., Ward, L., Humphrey, T.J. Jorgensen, F. (2008) Detection of Campylobacter jejuni and Campylobacter coli from broiler chicken-related samples using BAX PCR and conventional International Organization for Standardization culture. J Food Prot 71, 835 838.
42. Woldemariam, E., Bouma, A., Vernooij, J.C. Stegeman, A. (2008) The sensitivity and specificity of fecal and cecal culture for the detection of Campylobacter in Dutch broiler flocks quantified by Bayesian analysis. Int J Food Microbiol 121, 308 312.