Messenger RNA-based RT-PCR detection of viable Salmonella

International Dairy Journal

Volumn 12, Issue 2-3, 2002, Pages 233-238

  Rijpens, Nancy ^a   Jannes, Geert ^b   Herman, Lieve ^a  

^a Centrum voor Landbouwkundig Onderzoek Gent CLO / Centre de Recherches Agronomiques Gand   (Belgium)

^b INNOGENETICS   (Belgium)

Author keywords

MRNA; RT PCR; Salmonella

Indexed keywords

ENTEROBACTERIACEAE; SALMONELLA;

EID: 0036128379     PISSN: 09586946     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0958-6946(01)00158-3     Document Type: Conference Paper

References (23)

1. Detection of viable Legionella pneumophila in water by polymerase chain reaction and gene probe methods
2. A nucleic acid sequence-based amplification system for detection of Listeria monocytogenes hlyA sequences
3. Introduction of pAMβ1 into Listeria monocytogenes by conjugation and homology between native L. monocytogenes plasmids
4. Detection of viable and dead Listeria monocytogenes by PCR
5. Direct detection of Listeria monocytogenes in 25 milliliters of raw milk by a two-step PCR with nested primers
6. Physiological states of individual Salmonella typhimurium cells monitored by in situ reverse transcription-PCR
7. Polymerase chain reaction detection of nonviable bacterial pathogens
8. Sensitive detection of viable Listeria monocytogenes by reverse transcription-PCR
9. Detection of bacterial mRNA using polymerase chain reaction
10. Effect of stress treatments on the detection of Listeria monocytogenes and enterotoxigenic Escherichia coli by the polymerase chain reaction
11. rRNA stability in heat-killed and UV-irradiated enterotoxigenic Staphylococcus aureus and E. coli O157:H7
12. Detection of viable Listeria monocytogenes with a 5′nuclease PCR assay
13. Rapid detection of stressed Salmonella spp. in dairy and egg products using immunomagnetic separation and PCR
14. Direct detection of Brucella spp. in raw milk using PCR and reverse hybridisation with 16S-23S rRNA spacer probes
15. Detection of mRNA by reverse transcription-PCR as an indicator of viability in Escherichia coli cells
16. Effect of post-treatment holding conditions on detection of tufA mRNA in ethanol-treated Escherichia coli: Implications for RT-PCR based indirect viability tests
17. Dev-elopment of a NASBA-based protocol for detection of Salmonella in liquid whole egg
18. Effects of stress treatments on the detection of Salmonella typhimurium by in situ hybridization
19. Evaluation of the NASBA nucleic acid amplification system for assesment of the viability of Campylobacter jejuni
20. Direct detection of viable bacteria, molds and yeasts by reverse transcriptase PCR in contaminated milk samples after heat treatment
21. Assesment of mycobacterial viability by RNA amplification