Preliminary development and validation of a real-time reverse transcription PCR assay for the semi-quantification of viable Campylobacter jejuni in water samples

Water Science and Technology

Volumn 60, Issue 12, 2009, Pages 3151-3158

  Lin, S ^a   Gilpin, B ^a   Scholes, P ^a   Podivinsky, E ^a   Klena, J ^b   Savill, M ^a  

^a ESR Christchurch Science Centre   (New Zealand)

^b PSC452 ^*  (United States)

Author keywords

Campylobacter jejuni; Real time quantitative RT PCR; TaqMan; Viability; Water

Indexed keywords

CELL CULTURE; CLONING; DNA; GENES; RIVERS; RNA; TRANSCRIPTION;

AVERAGE RECOVERY; CAMPYLOBACTER JEJUNI; COLONY FORMING UNITS; CONTROL STANDARDS; DNA FRAGMENT; GENOME EQUIVALENT; HEAT-SHOCK; MESSENGER RNAS (MRNA); MOST PROBABLE NUMBER; MULTI-STEP; NATURAL RIVER; PCR METHOD; PLASMID DNA; REAL-TIME QUANTITATIVE RT-PCR; REAL-TIME REVERSE TRANSCRIPTION PCR ASSAYS; RIVER WATER; RIVER WATER SAMPLES; SEMI-QUANTITATIVE; VIABLE CELLS; WATER SAMPLES;

DYES;

MESSENGER RNA; RIVER WATER; BACTERIAL DNA; BACTERIAL RNA; CHAPERONIN;

BACTERIUM; BIOASSAY; DNA; GENOME; HEAT SHOCK; PLASMID; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; REAL TIME; RIVER WATER;

BACTERIAL CELL; BACTERIAL STRAIN; BACTERIAL VIABILITY; BACTERIUM ISOLATE; CAMPYLOBACTER JEJUNI; CELL VIABILITY; COLONY FORMING UNIT; CONFERENCE PAPER; CONTROLLED STUDY; GENE SEQUENCE; GENOME; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; VALIDATION PROCESS; WATER MANAGEMENT; WATER SAMPLING; ARTICLE; BIOSYNTHESIS; GENETICS; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MICROBIOLOGY; PLASMID; REPRODUCIBILITY; RIVER; STANDARD; TIME;

CAMPYLOBACTER JEJUNI;

CAMPYLOBACTER JEJUNI; CHAPERONIN 60; DNA, BACTERIAL; PLASMIDS; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RIVERS; RNA, BACTERIAL; RNA, MESSENGER; TIME FACTORS; WATER MICROBIOLOGY;

EID: 77649205641     PISSN: 02731223     EISSN: None     Source Type: Book Series    
DOI: 10.2166/wst.2009.811     Document Type: Article

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