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Volumn 827, Issue , 2012, Pages 253-270

High-throughput flow cytometry bead-based multiplex assay for identification of Rho GTPase inhibitors

Author keywords

Bead based multiplex assay; Cdc42; Flow cytometry; Fluorescent GTP binding; Rho GTPases; Screen

Indexed keywords

ENZYME INHIBITOR; RHO GUANINE NUCLEOTIDE BINDING PROTEIN;

EID: 84855905989     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-442-1_17     Document Type: Article
Times cited : (25)

References (20)
  • 1
    • 0034306013 scopus 로고    scopus 로고
    • Function of Rho family proteins in actin dynamics during phagocytosis and engulfment
    • Chimini, G., and Chavrier, P. (2000) Function of Rho family proteins in actin dynamics during phagocytosis and engulfment. Nature Cell Biol 2, E191-196.
    • (2000) Nature Cell Biol , vol.2
    • Chimini, G.1    Chavrier, P.2
  • 2
    • 0037069690 scopus 로고    scopus 로고
    • Rho GTPases in cell biology
    • DOI 10.1038/nature01148
    • Etienne-Manneville, S., and Hall, A. (2002) Rho GTPases in cell biology. Nature 420, 629-635. (Pubitemid 36764489)
    • (2002) Nature , vol.420 , Issue.6916 , pp. 629-635
    • Etienne-Manneville, S.1    Hall, A.2
  • 3
    • 0345731237 scopus 로고    scopus 로고
    • Cell migration: Rho GTPases lead the way
    • DOI 10.1016/j.ydbio.2003.06.003
    • Raftopoulou, M., and Hall, A. (2004) Cell migration: Rho GTPases lead the way. Dev Biol 265, 23-32. (Pubitemid 38018819)
    • (2004) Developmental Biology , vol.265 , Issue.1 , pp. 23-32
    • Raftopoulou, M.1    Hall, A.2
  • 4
    • 0037139607 scopus 로고    scopus 로고
    • The role of Rho GTPases in disease development
    • DOI 10.1016/S0378-1119(02)00426-2, PII S0378111902004262
    • Boettner, B., and Van Aelst, L. (2002) The role of Rho GTPases in disease development. Gene 286, 155-174. (Pubitemid 34273873)
    • (2002) Gene , vol.286 , Issue.2 , pp. 155-174
    • Boettner, B.1    Van Aelst, L.2
  • 5
    • 21244478650 scopus 로고    scopus 로고
    • Rho GTPase expression in tumourigenesis: Evidence for a significant link
    • DOI 10.1002/bies.20238
    • Gomez del Pulgar, T., Benitah, S.A., Valeron, P.F., Espina, C., and Lacal, J.C. (2005) Rho GTPase expression in tumourigenesis: evidence for a signifi cant link. Bioessays 27, 602-613. (Pubitemid 40896091)
    • (2005) BioEssays , vol.27 , Issue.6 , pp. 602-613
    • Gomez Del Pulgar, T.1    Benitah, S.A.2    Valeron, P.F.3    Espina, C.4    Lacal, J.C.5
  • 6
  • 7
    • 44449178868 scopus 로고    scopus 로고
    • Rho GTPases in cancer cell biology
    • Vega, F.M., and Ridley, A.J. (2008) Rho GTPases in cancer cell biology. FEBS Lett 582, 2093-2101.
    • (2008) FEBS Lett , vol.582 , pp. 2093-2101
    • Vega, F.M.1    Ridley, A.J.2
  • 10
    • 37249019970 scopus 로고    scopus 로고
    • Specificity and mechanism of action of EHT 1864, a novel small molecule inhibitor of Rac family small GTPases
    • DOI 10.1074/jbc.M703571200
    • Shutes, A., Onesto, C., Picard, V., Leblond, B., Schweighoffer, F., and Der, C.J. (2007) Specifi city and mechanism of action of EHT 1864, a novel small molecule inhibitor of Rac family small GTPases. J Biol Chem 282, 35666-35678. (Pubitemid 350277149)
    • (2007) Journal of Biological Chemistry , vol.282 , Issue.49 , pp. 35666-35678
    • Shutes, A.1    Onesto, C.2    Picard, V.3    Leblond, B.4    Schweighoffer, F.5    Der, C.J.6
  • 11
    • 77949351405 scopus 로고    scopus 로고
    • Identifi cation of a small GTPase inhibitor using a high-throughput flow cytometry bead-based multiplex assay
    • Surviladze, Z., Waller, A., Wu, Y., Romero, E., Edwards, B.S., Wandinger-Ness, A., and Sklar, L.A. (2010) Identifi cation of a small GTPase inhibitor using a high-throughput flow cytometry bead-based multiplex assay. J Biomol Screen 15, 10-20.
    • (2010) J Biomol Screen , vol.15 , pp. 10-20
    • Surviladze, Z.1    Waller, A.2    Wu, Y.3    Romero, E.4    Edwards, B.S.5    Wandinger-Ness, A.6    Sklar, L.A.7
  • 12
    • 0035311495 scopus 로고    scopus 로고
    • Fluorescent BODIPY-GTP analogs: Real-time measurement of nucleotide binding to G proteins
    • DOI 10.1006/abio.2001.5011
    • McEwen, D.P., Gee, K.R., Kang, H.C., and Neubig, R.R. (2001) Fluorescent BODIPYGTP analogs: real-time measurement of nucleotide binding to G proteins. Anal Biochem 291, 109-117. (Pubitemid 32324531)
    • (2001) Analytical Biochemistry , vol.291 , Issue.1 , pp. 109-117
    • McEwen, D.P.1    Gee, K.R.2    Kang, H.C.3    Neubig, R.R.4
  • 14
    • 61849108234 scopus 로고    scopus 로고
    • High-throughput screening for small-molecule inhibitors of LARG-stimulated RhoA nucleotide binding via a novel fl uorescence polarization assay
    • Evelyn, C.R., Ferng, T., Rojas, R.J., Larsen, M.J., Sondek, J., and Neubig, R.R. (2009) High-throughput screening for small-molecule inhibitors of LARG-stimulated RhoA nucleotide binding via a novel fl uorescence polarization assay. J Biomol Screen 14, 161-172.
    • (2009) J Biomol Screen , vol.14 , pp. 161-172
    • Evelyn, C.R.1    Ferng, T.2    Rojas, R.J.3    Larsen, M.J.4    Sondek, J.5    Neubig, R.R.6
  • 15
    • 38649123032 scopus 로고    scopus 로고
    • Advances in multiple analyte profi ling
    • Salas, V.M., Edwards, B.S., and Sklar, L.A. (2008) Advances in multiple analyte profi ling. Adv Clin Chem 45, 47-74.
    • (2008) Adv Clin Chem , vol.45 , pp. 47-74
    • Salas, V.M.1    Edwards, B.S.2    Sklar, L.A.3
  • 16
    • 34250678990 scopus 로고    scopus 로고
    • Biomolecular screening of formylpeptide receptor ligands with a sensitive, quantitative, high-throughput flow cytometry platform
    • Edwards, B.S., Young, S.M., Oprea, T.I., Bologa, C.G., Prossnitz, E.R., and Sklar, L.A. (2006) Biomolecular screening of formylpeptide receptor ligands with a sensitive, quantitative, high-throughput flow cytometry platform. Nature Protocols 1, 59-66.
    • (2006) Nature Protocols , vol.1 , pp. 59-66
    • Edwards, B.S.1    Young, S.M.2    Oprea, T.I.3    Bologa, C.G.4    Prossnitz, E.R.5    Sklar, L.A.6
  • 17
    • 79953214396 scopus 로고    scopus 로고
    • Microspherebased flow cytometry protease assays for use in protease activity detection and high-throughput screening
    • Saunders, M.J., Edwards, B.S., Zhu, J., Sklar, L.A., and Graves, S.W. (2010) Microspherebased flow cytometry protease assays for use in protease activity detection and high-throughput screening. Curr Protoc in Cytom Unit 13.12.1-17.
    • (2010) Curr Protoc in Cytom Unit , vol.13 , Issue.12 , pp. 1-17
    • Saunders, M.J.1    Edwards, B.S.2    Zhu, J.3    Sklar, L.A.4    Graves, S.W.5
  • 19
    • 0033003760 scopus 로고    scopus 로고
    • A simple statistical parameter for use in evaluation and validation of high throughput screening assays
    • DOI 10.1177/108705719900400206
    • Zhang, J. H., Chung, T. D., and Oldenburg, K. R. (1999) A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 4, 67-73. (Pubitemid 29278954)
    • (1999) Journal of Biomolecular Screening , vol.4 , Issue.2 , pp. 67-73
    • Zhang, J.-H.1    Chung, T.D.Y.2    Oldenburg, K.R.3
  • 20
    • 32344448950 scopus 로고    scopus 로고
    • Statistical practice in high-throughput screening data analysis
    • DOI 10.1038/nbt1186, PII NBT1186
    • Malo, N., Hanley, J.A., Cerquozzi, S., Pelletier, J., and Nadon, R. (2006) Statistical practice in high-throughput screening data analysis. Nature Biotech 24, 167-175. (Pubitemid 43221699)
    • (2006) Nature Biotechnology , vol.24 , Issue.2 , pp. 167-175
    • Malo, N.1    Hanley, J.A.2    Cerquozzi, S.3    Pelletier, J.4    Nadon, R.5


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.