Batch reactor performance for the enzymatic synthesis of cephalexin: Influence of catalyst enzyme loading and particle size

New Biotechnology

Volumn 29, Issue 2, 2012, Pages 218-226

  Valencia, Pedro ^a,b   Flores, Sebastián ^c   Wilson, Lorena ^b   Illanes, Andrés ^b  

^a DEPARTAMENTO DE FÍSICA   (Chile)

^b PONTIFICIA UNIVERSIDAD CATÓLICA DE VALPARAÍSO   (Chile)

^c UNIVERSITY OF CHILE   (Chile)

Author keywords

[No Author keywords available]

Indexed keywords

ACYL-ENZYME COMPLEX; CEPHALEXIN; CONTROLLED SYNTHESIS; CONVERSION YIELD; DIFFUSION COMPONENTS; DIFFUSION PARAMETERS; EFFECTIVE DIFFUSION COEFFICIENTS; ENZYMATIC SYNTHESIS; ENZYME LOADING; FICK'S LAW; FREE DIFFUSION; FREE ENZYME; IMMOBILIZED ENZYME; INTRINSIC KINETICS; KINETIC CONSTANT; NON-LINEAR RELATIONSHIPS; PENICILLIN ACYLASE; POROUS STRUCTURES; REACTING SPECIES; REACTION-DIFFUSION MODELS; REACTION-DIFFUSION PROCESS; REACTOR DESIGNS; REACTOR PERFORMANCE; SPECIFIC PRODUCTIVITY; VOLUMETRIC PRODUCTIVITY;

BATCH REACTORS; CATALYSTS; COMPUTER SIMULATION; DIFFUSION IN LIQUIDS; KINETICS; LOADING; MATHEMATICAL MODELS; PARTICLE SIZE; PARTICLE SIZE ANALYSIS; PRODUCTIVITY; RATE CONSTANTS; REACTION KINETICS; SYNTHESIS (CHEMICAL);

ENZYMES;

CEFALEXIN; PENICILLIN AMIDASE;

ARTICLE; BATCH REACTOR; CHEMICAL REACTION; CHEMICAL REACTION KINETICS; ENZYME IMMOBILIZATION; ENZYME SYNTHESIS; HYDROLYSIS; MATHEMATICAL MODEL; PARTICLE SIZE; PRIORITY JOURNAL; REACTOR DESIGN; VOLUMETRY;

CATALYSIS; CEPHALEXIN; COMPUTER SIMULATION; MODELS, CHEMICAL; PARTICLE SIZE; PENICILLIN AMIDASE;

EID: 84855818748     PISSN: 18716784     EISSN: 18764347     Source Type: Journal    
DOI: 10.1016/j.nbt.2011.09.002     Document Type: Article

References (20)

1. Bruggink A. Synthesis of β-Lactam Antibiotics. Chemistry, Biocatalysis and Process Integration 2001, Kluwer.
2. Kallenberg A., et al. Immobilization of penicillin G acylase: the key to optimum performance. Adv Synth Catal 2005, 347:905-926.
3. Schroën C., et al. Cephalexin synthesis by immobilized penicillin G acylase under non-isothermal conditions: reduction of diffusion limitations. J Mol Catal B: Enzym 2001, 15:163-172.
4. Fernández-Lafuente R., et al. Facile synthesis of artificial enzyme nano-environments via solid-phase chemistry of immobilized derivatives: dramatic stabilization of penicillin acylase versus organic solvents. Enzyme Microb Technol 1999, 24:96-103.
5. Montes T., et al. Improved stabilization of genetically modified penicillin G acylase in the presence of organic cosolvents by co-immobilization of the enzyme with polyethyleneimine. Adv Synth Catal 2007, 349:459-464.
6. Mateo C., et al. A new, mild cross-linking methodology to prepare cross-linked enzyme aggregates. Biotechnol Bioeng 2004, 86:273-276.
7. Engasser J.M., Horvath C. Effect of internal diffusion in heterogeneous enzyme systems: evaluation of true kinetic parameters and substrate diffusivity. J Theor Biol 1973, 42:137-155.
8. Spieß A., et al. pH Gradients in immobilized amidases and their influence on rates and yields of β-lactam hydrolysis. Biotechnol Bioeng 1999, 62:267-277.
9. Schroën C., et al. Modelling of the enzymatic kinetically controlled synthesis of cephalexin: influence of diffusion limitations. Biotechnol Bioeng 2002, 80:331-340.
10. Valencia P., et al. Evaluation of the incidence of diffusional restrictions on the enzymatic reactions of hydrolysis of penicillin G and synthesis of cephalexin. Enzyme Microb Technol 2010, 47:268-276.
11. Illanes A., et al. Production of cephalexin in organic medium at high substrate concentrations with CLEA of penicillin acylase and PGA-450. Enzyme Microb Technol 2007, 40:195-203.
12. Ospina S., et al. Characterization and use of penicillin acylase biocatalyst. J Chem Technol Biotechnol 1992, 53:205-213.
13. Shewale J., Sivaraman H. Penicillin acylase - enzyme production and its application in the manufacture of 6-APA. Proc Biochem 1989, 24:146-154.
14. Illanes A., et al. Synthesis of cephalexin with immobilized penicillin acylase at very high substrate concentrations in fully aqueous medium. J Mol Catal B: Enzym 2007, 47:72-78.
15. Van Langen L., et al. Active site titration as a tool for the evaluation of immobilization procedures of penicillin acylase. Biotechnol Bioeng 2002, 79:224-228.
16. Grunwald P. Determination of effective diffusion coefficients - an important parameter for the efficiency of immobilized biocatalysts. Biochem Educ 1989, 17:99-102.
17. Chapra S., Canale R. Numerical Methods for Engineers 2006, McGraw-Hill. 5th ed.
18. Levenspiel O. Chemical Reaction Engineering 1999, Wiley & Sons. 3rd ed.
19. Youshko M.I., et al. VKl Penicillin acylase-catalyzed synthesis of ampicillin in "aqueous solution-precipitate" system, high substrate concentration and supersaturation effect. J Mol Catal B: Enzym 2000, 10:509-515.
20. Youshko M.I., et al. Penicillin acylase-catalyzed synthesis of β-lactam antibiotics in highly condensed aqueous systems: beneficial impact of kinetic substrate supersaturation. Biotechnol Bioeng 2004, 8:323-329.