Proteomics paves the way for Q fever diagnostics

Genome Medicine

Volumn 3, Issue 7, 2011, Pages

  Kowalczewska, Malgorzata ^a   Sekeyová, Zuzana ^b   Raoult, Didier ^a  

^a AIX MARSEILLE UNIVERSITÉ   (France)

^b INSTITUTE OF VIROLOGY   (Slovakia)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL ENDOCARDITIS; BACTERIUM ISOLATE; COXIELLA BURNETII; GENE SEQUENCE; PNEUMONIA; PRIORITY JOURNAL; PROTEOMICS; Q FEVER;

EID: 84855695767     PISSN: None     EISSN: 1756994X     Source Type: Journal    
DOI: 10.1186/gm266     Document Type: Article

References (105)

1. Angelakis E, Raoult D. Q Fever. Vet Microbiol 2010, 140:297-309. 10.1016/j.vetmic.2009.07.016, 19875249.
2. Roest HI, Tilburg JJ, van der Hoek W, Vellema P, van Zijderveld FG, Klaassen CH. The Q fever epidemic in The Netherlands: history, onset, response and reflection. Epidemiol Infect 2010, 139:1-12.
3. Miceli MH, Veryser AK, Anderson AD, Hofinger D, Lee SA, Tancik C. A case of person-to-person transmission of Q fever from an active duty serviceman to his spouse. Vector Borne Zoonotic Dis 2010, 10:539-541. 10.1089/vbz.2009.0101, 20020811.
4. Carcopino X, Raoult D, Bretelle F, Boubli L, Stein A. Q Fever during pregnancy: a cause of poor fetal and maternal outcome. Ann N Y Acad Sci 2009, 1166:79-89. 10.1111/j.1749-6632.2009.04519.x, 19538266.
5. Frankel D, Richet H, Renvoise A, Raoult D. Q fever in France, 1985-2009. Emerg Infect Dis 2011, 17:350-356.
6. Enserink M. Infectious diseases. Questions abound in Q-fever explosion in The Netherlands. Science 2010, 327:266-267.
7. Klaassen CH, Nabuurs-Franssen MH, Tilburg JJ, Hamans MA, Horrevorts AM. Multigenotype Q fever outbreak, The Netherlands. Emerg Infect Dis 2009, 15:613-614. 10.3201/eid1504.081612, 2671457, 19331749.
8. Raoult D, Marrie T, Mege JL. Natural history and pathophysiology of Q fever. Lancet Infect Dis 2005, 5:219-226. 10.1016/S1473-3099(05)70052-9, 15792739.
9. Gimenez DF. Staining Rickettsiae in yolk-sac cultures. Stain Technol 1964, 39:135-140.
10. Seshadri R, Paulsen IT, Eisen JA, Read TD, Nelson KE, Nelson WC. Complete genome sequence of the Q-fever pathogen Coxiella burnetii. Proc Natl Acad Sci USA 2003, 100:5455-5460. 10.1073/pnas.0931379100, 154366, 12704232.
11. Beare PA, Porcella SF, Seshadri R, Samuel JE, Heinzen RA. Preliminary assessment of genome differences between the reference Nine Mile isolate and two human endocarditis isolates of Coxiella burnetii. Ann N Y Acad Sci 2005, 1063:64-67. 10.1196/annals.1355.007, 16481490.
12. Voth DE, Beare PA, Howe D, Sharma UM, Samoilis G, Cockrell DC. The Coxiella burnetii cryptic plasmid is enriched in genes encoding type IV secretion system substrates. J Bacteriol 2011, 193:1493-1503. 10.1128/JB.01359-10, 21216993.
13. Beare PA, Unsworth N, Andoh M, Voth DE, Omsland A, Gilk SD. Comparative genomics reveal extensive transposon-mediated genomic plasticity and diversity among potential effector proteins within the genus Coxiella. Infect Immun 2009, 77:642-656. 10.1128/IAI.01141-08, 2632050, 19047403.
14. Vadovic P, Fuleova A, Ihnatko R, Skultety L, Halada P, Toman R. Structural studies of lipid A from a lipopolysaccharide of the Coxiella burnetii isolate RSA 514 (Crazy). Clin Microbiol Infect 2009, 15:198-199.
15. Vadovic P, Slaba K, Fodorova M, Skultety L, Toman R. Structural and functional characterization of the glycan antigens involved in immunobiology of Q fever. Ann N Y Acad Sci 2005, 1063:149-153. 10.1196/annals.1355.023, 16481506.
16. Toman R, Skultety L, Ihnatko R. Coxiella burnetii glycomics and proteomics -- tools for linking structure to function. Ann N Y Acad Sci 2009, 1166:67-78. 10.1111/j.1749-6632.2009.04512.x, 19538265.
17. Skultety LHM, Flores-Ramirez G, Miernyk JA, Ciampor F, Toman R, Sekeyova Z. Proteomic comparaison of virulent phase I and avirulent phase II of Coxiella burnetii, the causautive agent of Q fever. J Proteomics 2011,
18. Musso D, Raoult D. Coxiella burnetii blood cultures from acute and chronic Q-fever patients. J Clin Microbiol 1995, 33:3129-3132. 228658, 8586687.
19. Skultety L, Hernychova L, Toman R, Hubalek M, Slaba K, Zechovska J. Coxiella burnetii whole cell lysate protein identification by mass spectrometry and tandem mass spectrometry. Ann N Y Acad Sci 2005, 1063:115-122. 10.1196/annals.1355.019, 16481502.
20. Skultety L, Hernychova L, Bereghazyova E, Slaba K, Toman R. Detection of specific spectral markers of Coxiella burnetii isolates by MALDI-TOF mass spectrometry. Acta Virol 2007, 51:55-58.
21. Maurin M, Raoult D. Q fever. Clin Microbiol Rev 1999, 12:518-553. 88923, 10515901.
22. Omsland A, Cockrell DC, Howe D, Fischer ER, Virtaneva K, Sturdevant DE. Host cell-free growth of the Q fever bacterium Coxiella burnetii. Proc Natl Acad Sci USA 2009, 106:4430-4434. 10.1073/pnas.0812074106, 2657411, 19246385.
23. Battisti JM, Hicks LD, Minnick MF. A unique Coxiella burnetii lipoprotein involved in metal-binding (LimB). Microbiology 2011,
24. Gilk SD, Beare PA, Heinzen RA. Coxiella burnetii expresses a functional Δ24 sterol reductase. J Bacteriol 2010, 192:6154-6159. 10.1128/JB.00818-10, 2981196, 20870767.
25. Textoris J, Ban LH, Capo C, Raoult D, Leone M, Mege JL. Sex-related differences in gene expression following Coxiella burnetii infection in mice: potential role of circadian rhythm. PLoS One 2010, 5:e12190. 10.1371/journal.pone.0012190, 2921390, 20730052.
26. Leone M, Honstettre A, Lepidi H, Capo C, Bayard F, Raoult D. Effect of sex on Coxiella burnetii infection: protective role of 17beta-estradiol. J Infect Dis 2004, 189:339-345. 10.1086/380798, 14722900.
27. Voth DE, Heinzen RA. Coxiella type IV secretion and cellular microbiology. Curr Opin Microbiol 2009, 12:74-80. 10.1016/j.mib.2008.11.005, 2670610, 19144560.
28. Luhrmann A, Nogueira CV, Carey KL, Roy CR. Inhibition of pathogen-induced apoptosis by a Coxiella burnetii type IV effector protein. Proc Natl Acad Sci USA 2010, 107:18997-19001. 10.1073/pnas.1004380107, 2973885, 20944063.
29. Chen C, Banga S, Mertens K, Weber MM, Gorbaslieva I, Tan Y. Large-scale identification and translocation of type IV secretion substrates by Coxiella burnetii. Proc Natl Acad Sci USA 2010, 107:21755-21760. 10.1073/pnas.1010485107, 3003115, 21098666.
30. Coleman SA, Fischer ER, Howe D, Mead DJ, Heinzen RA. Temporal analysis of Coxiella burnetii morphological differentiation. J Bacteriol 2004, 186:7344-7352. 10.1128/JB.186.21.7344-7352.2004, 523218, 15489446.
31. Coleman SA, Fischer ER, Cockrell DC, Voth DE, Howe D, Mead DJ. Proteome and antigen profiling of Coxiella burnetii developmental forms. Infect Immun 2007, 75:290-298. 10.1128/IAI.00883-06, 1828411, 17088354.
32. Voth DE, Beare PA, Howe D, Sharma UM, Samoilis G, Cockrell DC, Omsland A, Heinzen RA. The Coxiella burnetii cryptic plasmid enriched in genes encoding type IV secretion system. J Bacteriol 2011, 193:1493-1503. 10.1128/JB.01359-10, 21216993.
33. Samoilis G, Aivaliotis M, Vranakis I, Papadioti A, Tselentis Y, Tsiotis G. Proteomic screening for possible effector molecules secreted by the obligate intracellular pathogen Coxiella burnetii. J Proteome Res 2010, 9:1619-1626. 10.1021/pr900605q, 20044831.
34. Voth DE, Howe D, Beare PA, Vogel JP, Unsworth N, Samuel JE. The Coxiella burnetii ankyrin repeat domain-containing protein family is heterogeneous, with C-terminal truncations that influence Dot/Icm-mediated secretion. J Bacteriol 2009, 191:4232-4242. 10.1128/JB.01656-08, 2698476, 19411324.
35. Tissot-Dupont H, Raoult D. Q fever. Infect Dis Clin North Am 2008, 22:505-514. 10.1016/j.idc.2008.03.002, 18755387.
36. Imbert G, La Scola B. Diagnosis of Q fever using indirect microimmunofluorescence. Methods Mol Biol 2006, 345:197-202.
37. Fournier PE, Raoult D. Comparison of PCR and serology assays for early diagnosis of acute Q fever. J Clin Microbiol 2003, 41:5094-5098. 10.1128/JCM.41.11.5094-5098.2003, 262519, 14605144.
38. La Scola B. Current laboratory diagnosis of Q fever. Semin Pediatr Infect Dis 2002, 13:257-262. 10.1053/spid.2002.127199, 12491231.
39. Lepidi H, Casalta JP, Fournier PE, Habib G, Collart F, Raoult D. Quantitative histological examination of mechanical heart valves. Clin Infect Dis 2005, 40:655-661. 10.1086/427504, 15714409.
40. Fenollar F, Fournier PE, Raoult D. Molecular detection of Coxiella burnetii in the sera of patients with Q fever endocarditis or vascular infection. J Clin Microbiol 2004, 42:4919-4924. 10.1128/JCM.42.11.4919-4924.2004, 525147, 15528674.
41. Klee SR, Ellerbrok H, Tyczka J, Franz T, Appel B. Evaluation of a real-time PCR assay to detect Coxiella burnetii. Ann N Y Acad Sci 2006, 1078:563-565. 10.1196/annals.1374.111, 17114778.
42. Tilburg JJ, Nabuurs-Franssen MH, van Hannen EJ, Horrevorts AM, Melchers WJ, Klaassen CH. Contamination of commercial PCR master mix with DNA from Coxiella burnetii. J Clin Microbiol 2010, 48:4634-4635. 10.1128/JCM.00464-10, 3008432, 20962145.
43. Tilburg JJ, Melchers WJ, Pettersson AM, Rossen JW, Hermans MH, van Hannen EJ. Interlaboratory evaluation of different extraction and real-time PCR methods for detection of Coxiella burnetii DNA in serum. J Clin Microbiol 2010, 48:3923-3927. 10.1128/JCM.01006-10, 3020840, 20826645.
44. Dupont HT, Thirion X, Raoult D. Q fever serology: cutoff determination for microimmunofluorescence. Clin Diagn Lab Immunol 1994, 1:189-196. 368226, 7496944.
45. Tilburg JJ, Horrevorts AM, Peeters MF, Klaassen CH, Rossen JW. Identification by genotyping of a commercial antigen preparation as the source of a laboratory contamination with Q fever and as an unexpected rich source of control DNA. J Clin Microbiol 2010, 49:383-384. 3020417, 20980565.
46. Boden K, Wagner-Wiening C, Seidel T, Baier M, Bischof W, Straube E. Diagnosis of acute Q fever with emphasis on enzyme-linked immunosorbent assay and nested polymerase chain reaction regarding the time of serum collection. Diagn Microbiol Infect Dis 2010, 68:110-116. 10.1016/j.diagmicrobio.2010.06.001, 20846582.
47. Fournier PE, Marrie TJ, Raoult D. Diagnosis of Q fever. J Clin Microbiol 1998, 36:1823-1834. 104936, 9650920.
48. Li JS, Sexton DJ, Mick N, Nettles R, Fowler VG, Ryan T. Proposed modifications to the Duke criteria for the diagnosis of infective endocarditis. Clin Infect Dis 2000, 30:633-638. 10.1086/313753, 10770721.
49. Musso D, Raoult D. Serological cross-reactions between Coxiella burnetii and Legionella micdadei. Clin Diagn Lab Immunol 1997, 4:208-212. 170503, 9067657.
50. La Scola B, Raoult D. Serological cross-reactions between Bartonella quintana, Bartonella henselae, and Coxiella burnetii. J Clin Microbiol 1996, 34:2270-2274. 229230, 8862597.
51. Dupont HT, Brouqui P, Faugere B, Raoult D. Prevalence of antibodies to Coxiella burnetti, Rickettsia conorii, and Rickettsia typhi in seven African countries. Clin Infect Dis 1995, 21:1126-1133. 10.1093/clinids/21.5.1126, 8589132.
52. Gouriet F, Levy PY, Samson L, Drancourt M, Raoult D. Comparison of the new InoDiag automated fluorescence multiplexed antigen microarray to the reference technique in the serodiagnosis of atypical bacterial pneumonia. Clin Microbiol Infect 2008, 14:1119-1127. 10.1111/j.1469-0691.2008.02119.x, 19076843.
53. Gouriet F, Samson L, Delaage M, Mainardi JL, Meconi S, Drancourt M. Multiplexed whole bacterial antigen microarray, a new format for the automation of serodiagnosis: the culture-negative endocarditis paradigm. Clin Microbiol Infect 2008, 14:1112-1118. 10.1111/j.1469-0691.2008.02094.x, 19076842.
54. Skultety L, Hernychova L, Toman R, Kroca M, Stulik J, Macela A. Initial peptide mass fingerprinting analysis of proteins obtained by lysis of Coxiella burnetii cells. Acta Virol 2004, 48:29-33.
55. Pierce CY, Barr JR, Woolfitt AR, Moura H, Shaw EI, Thompson HA. Strain and phase identification of the U.S. category B agent Coxiella burnetii by matrix assisted laser desorption/ionization time-of-flight mass spectrometry and multivariate pattern recognition. Anal Chim Acta 2007, 583:23-31. 10.1016/j.aca.2006.09.065, 17386522.
56. Papadioti A, Markoutsa S, Vranakis I, Tselentis Y, Karas M, Psaroulaki A. A proteomic approach to investigate the differential antigenic profile of two Coxiella burnetii strains. J Proteomics 2011, 74:1150-1159. 10.1016/j.jprot.2011.04.016, 21565289.
57. Sekeyova Z, Kowalczewska M, Decloquement P, Pelletier N, Spitalska E, Raoult D. Identification of protein candidates for the serodiagnosis of Q fever endocarditis by an immunoproteomic approach. Eur J Clin Microbiol Infect Dis 2009, 28:287-295. 10.1007/s10096-008-0621-4, 18797945.
58. Sekeyova Z, Kowalczewska M, Vincentelli R, Decloquement P, Flores-Ramirez G, Skultety L. Characterization of antigens for Q fever serodiagnostics. Acta Virol 2010, 54:173-180. 10.4149/av_2010_03_173, 20822309.
59. Flores-Ramirez G, Toman R, Sekeyova Z, Skultety L. In silico prediction and identification of outer membrane proteins and lipoproteins from Coxiella burnetii by the mass spectrometry techniques. Clin Microbiol Infect 2009, 15:196-197.
60. Seng P, Drancourt M, Gouriet F, La Scola B, Fournier PE, Rolain JM. Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Infect Dis 2009, 49:543-551. 10.1086/600885, 19583519.
61. Murray PR. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry: usefulness for taxonomy and epidemiology. Clin Microbiol Infect 2010, 16:1626-1630. 10.1111/j.1469-0691.2010.03364.x, 20825435.
62. Hernychova L, Toman R, Ciampor F, Hubalek M, Vackova J, Macela A. Detection and identification of Coxiella burnetii based on the mass spectrometric analyses of the extracted proteins. Anal Chem 2008, 80:7097-7104. 10.1021/ac800788k, 18707130.
63. Zhang G, To H, Russell KE, Hendrix LR, Yamaguchi T, Fukushi H. Identification and characterization of an immunodominant 28-kilodalton Coxiella burnetii outer membrane protein specific to isolates associated with acute disease. Infect Immun 2005, 73:1561-1567. 10.1128/IAI.73.3.1561-1567.2005, 1064944, 15731054.
64. Hendrix LR, Mallavia LP, Samuel JE. Cloning and sequencing of Coxiella burnetii outer membrane protein gene com1. Infect Immun 1993, 61:470-477. 302752, 8423075.
65. Chao CC, Chen HW, Li X, Xu WB, Hanson B, Ching WM. Identification, cloning, and expression of potential diagnostic markers for Q fever. Ann N Y Acad Sci 2005, 1063:76-78. 10.1196/annals.1355.010, 16481493.
66. Beare PA, Chen C, Bouman T, Pablo J, Unal B, Cockrell DC. Candidate antigens for Q fever serodiagnosis revealed by immunoscreening of a Coxiella burnetii protein microarray. Clin Vaccine Immunol 2008, 15:1771-1779. 10.1128/CVI.00300-08, 2593175, 18845831.
67. Chen C, Bouman TJ, Beare PA, Mertens K, Zhang GQ, Russell-Lodrigue KE. A systematic approach to evaluate humoral and cellular immune responses to Coxiella burnetii immunoreactive antigens. Clin Microbiol Infect 2009, 15:156-157. 2916703, 19281461.
68. Deringer JR, Chen C, Samuel JE, Brown WC. Immunoreactive Coxiella burnetii Nine Mile proteins separated by 2D electrophoresis and identified by tandem mass spectrometry. Microbiology 2010, 157:526-542.
69. Vigil A, Ortega R, Nakajima-Sasaki R, Pablo J, Molina DM, Chao CC. Genome-wide profiling of humoral immune response to Coxiella burnetii infection by protein microarray. Proteomics 2010, 10:2259-2269. 10.1002/pmic.201000064, 2892821, 20391532.
70. Bini L, Sanchez-Campillo M, Santucci A, Magi B, Marzocchi B, Comanducci M. Mapping of Chlamydia trachomatis proteins by immobiline-polyacrylamide two-dimensional electrophoresis: spot identification by N-terminal sequencing and immunoblotting. Electrophoresis 1996, 17:185-190. 10.1002/elps.1150170130, 8907538.
71. Birkelund S, Bini L, Pallini V, Sanchez-Campillo M, Liberatori S, Clausen JD. Characterization of Chlamydia trachomatis l2-induced tyrosine-phosphorylated HeLa cell proteins by two-dimensional gel electrophoresis. Electrophoresis 1997, 18:563-567. 10.1002/elps.1150180338, 9150942.
72. Sanchez-Campillo M, Bini L, Comanducci M, Raggiaschi R, Marzocchi B, Pallini V. Identification of immunoreactive proteins of Chlamydia trachomatis by Western blot analysis of a two-dimensional electrophoresis map with patient sera. Electrophoresis 1999, 20:2269-2279. 10.1002/(SICI)1522-2683(19990801)20:11<2269::AID-ELPS2269>3.0.CO;2 -D, 10493131.
73. Jungblut PR, Schiele F, Zimny-Arndt U, Ackermann R, Schmid M, Lange S. Helicobacter pylori proteomics by 2-DE/MS, 1-DE-LC/MS and functional data mining. Proteomics 2010, 10:182-193. 10.1002/pmic.200900361, 19941309.
74. Bumann D, Jungblut PR, Meyer TF. Helicobacter pylori vaccine development based on combined subproteome analysis. Proteomics 2004, 4:2843-2848. 10.1002/pmic.200400909, 15378757.
75. Bernarde C, Khoder G, Lehours P, Burucoa C, Fauchere JL, Delchier JC. Proteomic Helicobacter pylori biomarkers discriminative of low-grade gastric MALT lymphoma and duodenal ulcer. Proteomics Clin Appl 2009, 3:672-681. 10.1002/prca.200800158, 21136979.
76. Bernarde C, Lehours P, Lasserre JP, Castroviejo M, Bonneu M, Megraud F. Complexomics study of two Helicobacter pylori strains of two pathological origins: potential targets for vaccine development and new insight in bacteria metabolism. Mol Cell Proteomics 2010, 9:2796-2826. 10.1074/mcp.M110.001065, 20610778.
77. Mullaney E, Brown PA, Smith SM, Botting CH, Yamaoka YY, Terres AM. Proteomic and functional characterization of the outer membrane vesicles from the gastric pathogen Helicobacter pylori. Proteomics Clin Appl 2009, 3:785-796. 10.1002/prca.200800192, 21136987.
78. Havlasova J, Hernychova L, Halada P, Pellantova V, Krejsek J, Stulik J. Mapping of immunoreactive antigens of Francisella tularensis live vaccine strain. Proteomics 2002, 2:857-867. 10.1002/1615-9861(200207)2:7<857::AID-PROT857>3.0.CO;2-L, 12124931.
79. Havlasova J, Hernychova L, Brychta M, Hubalek M, Lenco J, Larsson P. Proteomic analysis of anti-Francisella tularensis LVS antibody response in murine model of tularemia. Proteomics 2005, 5:2090-2103. 10.1002/pmic.200401123, 15892173.
80. Twine S, Bystrom M, Chen W, Forsman M, Golovliov I, Johansson A. A mutant of Francisella tularensis strain SCHU S4 lacking the ability to express a 58-kilodalton protein is attenuated for virulence and is an effective live vaccine. Infect Immun 2005, 73:8345-8352. 10.1128/IAI.73.12.8345-8352.2005, 1307091, 16299332.
81. Twine SM, Mykytczuk NC, Petit M, Tremblay TL, Lanthier P, Conlan JW. Francisella tularensis proteome: low levels of ASB-14 facilitate the visualization of membrane proteins in total protein extracts. J Proteome Res 2005, 4:1848-1854. 10.1021/pr050102u, 16212441.
82. Twine SM, Petit MD, Shen H, Mykytczuk NC, Kelly JF, Conlan JW. Immunoproteomic analysis of the murine antibody response to successful and failed immunization with live anti-Francisella vaccines. Biochem Biophys Res Commun 2006, 346:999-1008. 10.1016/j.bbrc.2006.06.008, 16781667.
83. Zhao G, Zhu L, Feng E, Cao X, Shang N, Liu X. A novel anti-virulence gene revealed by proteomic analysis in Shigella flexneri 2a. Proteome Sci 2010, 8:30. 10.1186/1477-5956-8-30, 2904734, 20540790.
84. Pieper R, Zhang Q, Parmar PP, Huang ST, Clark DJ, Alami H. The Shigella dysenteriae serotype 1 proteome, profiled in the host intestinal environment, reveals major metabolic modifications and increased expression of invasive proteins. Proteomics 2009, 9:5029-5045. 10.1002/pmic.200900196, 3001339, 19813213.
85. Kowalczewska M, Fenollar F, Lafitte D, Raoult D. Identification of candidate antigen in Whipple's disease using a serological proteomic approach. Proteomics 2006, 6:3294-3305. 10.1002/pmic.200500171, 16637011.
86. Kowalczewska M, Raoult D. Advances in Tropheryma whipplei research: the rush to find biomarkers for Whipple's disease. Future Microbiol 2007, 2:631-642. 10.2217/17460913.2.6.631, 18041904.
87. Eberhardt C, Engelmann S, Kusch H, Albrecht D, Hecker M, Autenrieth IB. Proteomic analysis of the bacterial pathogen Bartonella henselae and identification of immunogenic proteins for serodiagnosis. Proteomics 2009, 9:1967-1981. 10.1002/pmic.200700670, 19333998.
88. Saisongkorh W, Kowalczewska M, Azza S, Decloquement P, Rolain JM, Raoult D. Identification of candidate proteins for the diagnosis of Bartonella henselae infections using an immunoproteomic approach. FEMS Microbiol Lett 2010, 310:158-167. 10.1111/j.1574-6968.2010.02058.x, 20695898.
89. Minnick MF, Heinzen RA, Frazier ME, Mallavia LP. Characterization and expression of the cbbE' gene of Coxiella burnetii. J Gen Microbiol 1990, 136:1099-1107.
90. Cruz-Fisher MI, Cheng C, Sun G, Pal S, Teng A, Molina DM. Identification of immunodominant antigens by probing a whole Chlamydia ORFome microarray using sera from immunized mice. Infect Immun 2010, 79:246-257. 3019893, 20956570.
91. Wang J, Zhang Y, Lu C, Lei L, Yu P, Zhong G. A genome-wide profiling of the humoral immune response to Chlamydia trachomatis infection reveals vaccine candidate antigens expressed in humans. J Immunol 2010, 185:1670-1680. 10.4049/jimmunol.1001240, 20581152.
92. Felgner PL, Kayala MA, Vigil A, Burk C, Nakajima-Sasaki R, Pablo J. A Burkholderia pseudomallei protein microarray reveals serodiagnostic and cross-reactive antigens. Proc Natl Acad Sci USA 2009, 106:13499-13504. 10.1073/pnas.0812080106, 2717108, 19666533.
93. Vigil A, Ortega R, Jain A, Nakajima-Sasaki R, Tan X, Chomel BB. Identification of the feline humoral immune response to Bartonella henselae infection by protein microarray. PLoS One 2010, 5:e11447. 10.1371/journal.pone.0011447, 2897887, 20625509.
94. Samoilis G, Psaroulaki A, Vougas K, Tselentis Y, Tsiotis G. Analysis of whole cell lysate from the intercellular bacterium Coxiella burnetii using two gel-based protein separation techniques. J Proteome Res 2007, 6:3032-3041. 10.1021/pr070077n, 17602512.
95. Zhang YX, Zhi N, Yu SR, Li QJ, Yu GQ, Zhang X. Protective immunity induced by 67 K outer membrane protein of phase I Coxiella burnetii in mice and guinea pigs. Acta Virol 1994, 38:327-332.
96. Jaspers U, Thiele D, Krauss H. Monoclonal antibody based competitive ELISA for the detection of specific antibodies against Coxiella burnetii in sera from different animal species. Zentralbl Bakteriol 1994, 281:61-66.
97. Sekeyova Z, Kovacova E, Kazar J, Toman R, Olvecka S. Monoclonal antibodies to Coxiella burnetii that cross-react with strain Nine Mile. Clin Diagn Lab Immunol 1995, 2:531-534. 170195, 8548530.
98. McCaul TF, Banerjee-Bhatnagar N, Williams JC. Antigenic differences between Coxiella burnetii cells revealed by postembedding immunoelectron microscopy and immunoblotting. Infect Immun 1991, 59:3243-3253. 258159, 1715326.
99. Banerjee-Bhatnagar N, Bolt CR, Williams JC. Pore-forming activity of Coxiella burnetii outer membrane protein oligomer comprised of 29.5- and 31-kDa polypeptides. Inhibition of porin activity by monoclonal antibodies 4E8 and 4D6. Ann N Y Acad Sci 1996, 791:378-401. 10.1111/j.1749-6632.1996.tb53545.x, 8784519.
100. Sekeyova Z, Kovacova E. Identification and characterization of Coxiella burnetii strains and isolates using monoclonal antibodies. Ann N Y Acad Sci 2006, 1078:557-560. 10.1196/annals.1374.109, 17114776.
101. Raoult D, Laurent JC, Mutillod M. Monoclonal antibodies to Coxiella burnetii for antigenic detection in cell cultures and in paraffin-embedded tissues. Am J Clin Pathol 1994, 101:318-320.
102. Malou N, Raoult D. Immuno-PCR: a promising ultrasensitive diagnostic method to detect antigens and antibodies. Trends Microbiol 2011, 19:295-302. 10.1016/j.tim.2011.03.004, 21478019.
103. Sano T, Smith CL, Cantor CR. Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA conjugates. Science 1992, 258:120-122. 10.1126/science.1439758, 1439758.
104. Sparbier K, Wenzel T, Dihazi H, Blaschke S, Muller GA, Deelder A. Immuno-MALDI-TOF MS: new perspectives for clinical applications of mass spectrometry. Proteomics 2009, 9:1442-1450. 10.1002/pmic.200800616, 19235164.
105. Beare PA, Howe D, Cockrell DC, Omsland A, Hansen B, Heinzen RA. Characterization of a Coxiella burnetii ftsZ mutant generated by Himar1 transposon mutagenesis. J Bacteriol 2009, 191:1369-1381. 10.1128/JB.01580-08, 2648191, 19114492.