Isolation of Metarhizium anisopliae carboxypeptidase A with native disulfide bonds from the cytosol of Escherichia coli BL21(DE3)

Protein Expression and Purification

Volumn 82, Issue 1, 2012, Pages 116-124

  Austin, Brian P ^a   Waugh, David S ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Carboxypeptidase; DsbA; DsbC; gor; His tag; Maltose binding protein; MeCPA; Polyhistidine tag; Protein disulfide isomerase; Thermolysin; trxB

Indexed keywords

CARBOXYPEPTIDASE A; DISULFIDE; ESCHERICHIA COLI PROTEIN; HYBRID PROTEIN; MALTOSE BINDING PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BACULOVIRUS; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METARHIZIUM; MOLECULAR GENETICS; SOLUBILITY;

AMINO ACID SEQUENCE; BACULOVIRIDAE; CARBOXYPEPTIDASES A; DISULFIDES; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION; GENETIC VECTORS; MALTOSE-BINDING PROTEINS; METARHIZIUM; MOLECULAR SEQUENCE DATA; RECOMBINANT FUSION PROTEINS; SOLUBILITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; HEXAPODA; MAMMALIA; METARHIZIUM ANISOPLIAE;

EID: 84855487625     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.11.015     Document Type: Article

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