Isolation of carcinoembryonic antigen N-terminal domains (N-A1) from soluble aggregates

Protein Expression and Purification

Volumn 78, Issue 1, 2011, Pages 78-85

  Czepczyńska Krȩzel, Hanna ^a   Czerwinski, Marcin ^a,b   Krȩzel, Artur ^c   Krop Watorek, Anna ^a,d  

^a INSTITUTE OF IMMUNOLOGY AND EXPERIMENTAL THERAPY   (Poland)

^b OPOLE UNIVERSITY OF TECHNOLOGY   (Poland)

^c UNIVERSITY OF WROC AW   (Poland)

^d UNIVERSITY OF OPOLE   (Poland)

Author keywords

Aggregation; Carcinoembryonic antigen (CEA); Ig like domain; Maltose binding protein (MBP)

Indexed keywords

CARCINOEMBRYONIC ANTIGEN; DISULFIDE; GUANIDINE; HYBRID PROTEIN; MALTOSE BINDING PROTEIN;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; CIRCULAR DICHROISM; ESCHERICHIA COLI; GEL CHROMATOGRAPHY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN STABILITY; PROTEIN TERTIARY STRUCTURE; SOLUBILITY;

CARCINOEMBRYONIC ANTIGEN; CHROMATOGRAPHY, GEL; CIRCULAR DICHROISM; DISULFIDES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GUANIDINE; HUMANS; MALTOSE-BINDING PROTEINS; PROTEIN STABILITY; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; SOLUBILITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; TOBACCO ETCH VIRUS;

EID: 79955588400     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.03.014     Document Type: Article

References (34)

1. N. Beauchemin, P. Draber, G. Dveksler, P. Gold, S. Gray-Owen, F. Grunert, S. Hammarström, K.V. Holmes, A. Karlsson, M. Kuroki, S.H. Lin, L. Lucka, S.M. Najjar, M. Neumaier, B. Obrink, J.E. Shively, K.M. Skubitz, C.P. Stanners, P. Thomas, J.A. Thompson, M. Virji, S. Von Kleist, C. Wagener, S. Watt, W. Zimmermann, Redefined nomenclature for members of the carcinoembryonic antigen family, Exp. Cell Res. 252 (1999) 243-249.
2. W. Zimmermann, B. Ortlieb, R. Friedrich, S. von Kleist, Isolation and characterization of cDNA clones encoding the human carcinoembryonic antigen reveal a highly conserved repeating structure, Proc. Natl. Acad. Sci. USA 84 (1987) 2960-2964. (Pubitemid 17064758)
3. N. Beauchemin, S. Benchimol, D. Cournoyer, A. Fuks, C.P. Stanners, Isolation and characterization of full-length functional cDNA clones for human carcinoembryonic antigen, Mol. Cell. Biol. 7 (1987) 3221-3230. (Pubitemid 17134317)
4. P. Thomas, H. Hayashi, R. Zimmer, R.A. Forse, Regulation of cytokine production in carcinoembryonic antigen stimulated Kupffer cells by beta-2 adrenergic receptors: implications for hepatic metastasis, Cancer Lett. 209 (2004) 251-257. (Pubitemid 38678654)
5. R.N. Samara, L.M. Laguinge, J.M. Jessup, Carcinoembryonic antigen inhibits anoikis in colorectal carcinoma cells by interfering with TRAIL-R2 (DR5) signaling, Cancer Res. 67 (2007) 4774-4782. (Pubitemid 46910185)
6. R. Schoffelen, W.T. van der Graaf, G. Franssen, R.M. Sharkey, D.M. Goldenberg, W.J. McBride, E.A. Rossi, A. Eek, W.J. Oyen, O.C. Boerman, Terapy of carcinoembryonic antigen-expressing human colonic tumors in mice, J. Nucl. Med. 51 (2010) 1780-1787.
7. S. Benchimol, A. Fuks, S. Jothy, N. Beauchemin, K. Shirota, C.P. Stanners, Carcinoembryonic antigen, a human tumor marker, functions as an intercellular adhesion molecule, Cell 57 (1989) 327-334.
8. N. Stern-Ginossar, S. Nedvetzki, G. Markel, R. Gazit, G. Betser-Cohen, H. Achdout, M. Aker, R.S. Blumberg, D.M. Davis, B. Appelmelk, O. Mandelboim, Intercellular transfer of carcinoembryonic antigen from tumor cells to NK cells, J. Immunol. 179 (2007) 4424-4434.
9. L. Shao, M. Allez, M.S. Park, L. Mayer, Immunomodulatory roles of the carcinoembryonic antigen family of glycoproteins, Ann. N. Y. Acad. Sci. 1072 (2006) 194-209. (Pubitemid 44448453)
10. M.B. Bernstein, M. Chakraborty, E.K. Wansley, Z. Guo, A. Franzusoff, S. Mostböck, H. Sabzevari, J. Schlom, J.W. Hodge, Recombinant Saccharomyces cerevisiae (yeast-CEA) as a potent activator of murine dendritic cells, Vaccine 26 (2008) 509-521.
11. M. Virji, D. Evans, J. Griffith, D. Hill, L. Serino, A. Hadfield, S.M. Watt, Carcinoembryonic antigens are targeted by diverse strains of typable and non-typable Haemophilus influenzae, Mol. Microbiol. 36 (2000) 784-795.
12. M.P. Bos, D. Kao, D.M. Hogan, C.C.R. Grant, R.J. Belland, Carcinoembryonic antigen family receptor recognition by gonococcal Opa proteins requires distinct combinations of hypervariable Opa protein domains, Infect Immun. 70 (2002) 1715-1723. (Pubitemid 34242171)
13. K. Kuespert, S. Weibel, C.R. Hauck, Profiling of bacterial adhesin-host receptor recognition by soluble immunoglobulin superfamily domains, J. Microbiol. Methods 68 (2007) 478-485. (Pubitemid 46329150)
14. A. Krop-Wa̧torek, S. Oikawa, Y. Oyama, H. Nakazato, Oligomerization of N-terminal domain of carcinoembryonic antigen (CEA), expressed in Escherichia coli, Biochem. Biophys. Res. Commun. 242 (1998) 79-83. (Pubitemid 28412380)
15. N. Korotkova, Y. Yang, I. Le Trong, E. Cota, B. Demeler, J. Marchants, W.E. Thomas, R.E. Stenkamp, S.L. Moseley, S. Matthews, Binding of Dr adhesins of Escherichia coli to carcinoembryonic antigen triggers receptor dissociation, Mol. Microbiol. 67 (2008) 420-434.
16. S.M. Watt, A.M. Teixeira, G.Q. Zhou, R. Doyonnas, Y. Zhang, F. Grunert, R.S. Blumberg, M. Kuroki, K.M. Skubitz, P.A. Bates, Homophilic adhesion of human CEACAM1 involves N-terminal domain interactions: structural analysis of the binding site, Blood 98 (2001) 1469-1479.
17. J.M. Jessup, J.C. Kim, P. Thomas, S. Ishii, R. Ford, J.E. Shively, H. Durbin, C.P. Stanners, A. Fuks, H. Zhou, H.J. Hansen, D.M. Goldenberg, G. Steele Jr, Adhesion to carcinoembryonic antigen by human colorectal carcinoma cells involves at least two epitopes, Int. J. Cancer 55 (1993) 262-268. (Pubitemid 23296026)
18. P.A. Bates, J. Luo, M.J.E. Sternberg, A predicted three-dimensional structure for the carcinoembryonic antigen (CEA), FEBS Lett. 301 (1992) 207-214.
19. S. Oikawa, H. Nakazato, G. Kosaki, Primary structure of carcinoembryonic antigen (CEA) deduced from cDNA sequence, Biochem. Biophys. Res. Commun. 142 (1987) 511-518. (Pubitemid 17003376)
20. L.J. Lucast, R.T. Batey, J.A. Doudna, Large-scale purification of a stable form of recombinant tobacco etch virus protease, BioTechniques 30 (2000) 17-24.
21. U.K. Laemlli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227 (1970) 680-685.
22. M.M. Bradford, A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72 (1976) 248-254.
23. N.J. Greenfield, Using circular dichroism spectra to estimate protein secondary structure, Nat. Protoc. 1 (2006) 2876-2890.
24. A. Krȩżel, R. Latajka, G.D. Bujacz, W. Bal, Coordination properties of tris(2- carboxyethyl) phosphine, a newly introduced thiol reductant, and its oxide, Inorg. Chem. 42 (2003) 1994-2003.
25. G.L. Ellman, A colorimetric method for determining low concentrations of mercaptans, Arch. Biochem. Biophys. 74 (1958) 443-450.
26. J.A. Thompson, H. Pande, R.J. Paxton, L. Shively, A. Padma, R.L. Simmer, C.W. Todd, A.D. Riggs, J.E. Shively, Molecular cloning of a gene belonging to the carcinoembryonic antigen gene family and discussion of a domain model, Proc. Natl. Acad. Sci. USA 84 (1987) 2965-2969. (Pubitemid 17064759)
27. S. Altschul, W. Gish, Local alignment statistics, Meth. Enzymol. 266 (1996) 460-580.
28. L.J. McGuffin, K. Bryson, D.T. Jones, The PSIPRED protein structure prediction server, Bioinformatics 16 (2000) 404-405. (Pubitemid 30417087)
29. R.B. Kapust, D.S. Waugh, Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused, Protein Sci. 8 (1999) 1668-1674. (Pubitemid 29379949)
30. Y. Nominé, T. Ristriani, C. Laurent, J.F. Lefèvre, E. Weiss, G. Travé, Formation of soluble inclusion bodies by hpv e6 oncoprotein fused to maltose-binding protein, Protein Expr. Purif. 23 (2001) 22-32.
31. G.Q. Chen, J.E. Gouaux, Overexpression of bacterio-opsin in Escherichia coli as a water-soluble fusion to maltose binding protein: efficient regeneration of the fusion protein and selective cleavage with trypsin, Protein Sci. 5 (1996) 456-467. (Pubitemid 26081623)
32. A. Krop-Watorek, A.G. Kłopocki, M. Czerwinski, E. Lisowska, Adhesive properties of carcinoembryonic antigen glycoforms expressed in glycosylation-deficient Chinese hamster ovary cell lines, Acta Biochim. Pol. 49 (2002) 273-283.
33. J.C. Spurlino, G.Y. Lu, F.A. Quiocho, The 2.3-A resolution structure of the maltose- or maltodextrin-binding protein, a primary receptor of bacterial active transport and chemotaxis, J. Biol. Chem. 266 (1991) 5202-5219. (Pubitemid 21909478)
34. J. Buchner, R. Rudolph, Renaturation, purification and characterization of recombinant Fab-fragments produced in Escherichia coli, Nat. Biotechnol. 9 (1991) 157-162.