Improved recovery of active GST-fusion proteins from insoluble aggregates: Solubilization and purification conditions using PKM2 and HtrA2 as model proteins

BMB Reports

Volumn 44, Issue 4, 2011, Pages 279-284

  Park, Dae Wook ^a   Kim, Sang Soo ^a   Nam, Min Kyung ^a   Kim, Goo Young ^a   Kim, Jungho ^b   Rhim, Hyangshuk ^a  

^a The Catholic University of Korea   (South Korea)

^b Sogang University   (South Korea)

Author keywords

Insoluble GST fusion protein; Prokaryotic expression system; Sarkosyl; Solubilization; Triton X 100

Indexed keywords

GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; MITOCHONDRIAL PROTEIN; OCTOXINOL; PYRUVATE KINASE; SERINE PROTEINASE; SERINE PROTEINASE OMI;

ANIMAL; ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOUSE; SOLUBILITY;

ANIMALS; GLUTATHIONE TRANSFERASE; MICE; MITOCHONDRIAL PROTEINS; MODELS, MOLECULAR; OCTOXYNOL; PYRUVATE KINASE; RECOMBINANT FUSION PROTEINS; SERINE ENDOPEPTIDASES; SOLUBILITY;

PROKARYOTA;

EID: 79956055874     PISSN: 19766696     EISSN: 1976670X     Source Type: Journal    
DOI: 10.5483/BMBRep.2011.44.4.279     Document Type: Article

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