The genes lepA and hemN form a bicistronic operon in Bacillus subtilis

Microbiology

Volumn 142, Issue 7, 1996, Pages 1641-1649

  Homuth, Georg ^a   Heinemann, Marianne ^a   Zuber, Ulrich ^a   Schumann, Wolfgang ^a  

^a UNIVERSITY OF BAYREUTH   (Germany)

Author keywords

Bacillus subtilis; Bicistronic operon; hemN; lepA; Riboprobe

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL GENE; CISTRON; CONTROLLED STUDY; DNA FLANKING REGION; NONHUMAN; NORTHERN BLOTTING; OPERON; PRIORITY JOURNAL; TRANSCRIPTION TERMINATION;

EID: 0029949534     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/13500872-142-7-1641     Document Type: Article

References (33)

1. Beall, B. & Lutkenhaus, J. (1989). Nucleotide sequence and insertional inactivation of a bacillus subtilis gene that affects cell division, sporulation, and temperature sensitivity. J. Bacteriol 171, 6821-6834.
2. Birnboim, H. C. & Doly, J. (1979). A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7, 1513-1523.
3. Black, M. T., Munn, G. R. & Allsop, A. E. (1992). On the catalytic mechanism of prokaryotic leader peptidase. Biochem J 282, 539-543.
4. Coomber, S. A., Jones, R. M., Jordan, P. M. & Hunter, C. N. (1992). A putative anaerobic coproporphyrinogen III oxidase in Rhodobacter sphaeroides. I. Molecular cloning, transposon mutagenesis and sequence analysis of the gene. Mol Microbiol 6, 3155-3169.
5. Dibb, N. J. & Wolfe, P. B. (1986). lep operon proximal gene is not required for growth or secretion by Escherichia coli. J Bacteriol 16, 83-87.
6. van Dijl, J. M., van den Bergh, R., Reversma, T., Smith, H., Bron, S. & Venema, G. (1990). Molecular cloning of the Salmonella typhimurium lep gene in Escherichia coli. Mol Gen Genet 223, 233-240.
7. Dubnau, D. & Davidoff-Abelson, R. (1971). Fate of transforming DNA, following uptake by competent Bacillus subtilis. I. Formation and properties of the donor-recipient complex. J Mol Biol 56, 209-221.
8. Fleischmann, R. D., Adams, M. D., White, O., Clayton, R. A., Kirkness, E. F., Kerlavage, A. R., Bult, C. J., Tomb, J.-F., Dougherty, B. A., Merrick, J. M., McKenney, K., Sutton, G., FitzHugh, W., Fields, C., Gocayne, J. D., Scott, J., Shirley, R., Liu, L.-I., Glodek, A., Kelley, J. M., Weidman, J. F., Phillips, C. A., Spriggs, T. & Hedblom, E. (1995). Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269, 496-512.
9. Grunstein, M. & Wallis, J. (1979). Colony hybridization. Methods Enzymol 68, 379-389.
10. Haldenwang, W. G. (1995). The sigma factors of Bacillus subtilis. Microbiol Rev 59, 1-30.
11. Hecker, M., Schumann, W. & Völker, U. (1996). Heat-shock and general stress response in Bacillus subtilis. Mol Microbiol 19 417-428.
12. Hochuli, E., Bannwarth, W., Dobeli, H., Gentz, R. & Stüber, D. (1988). Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate absorbent. Bio/Technology 6, 1321-1325.
13. Horinouchi, S. & Weisblum, B. (1982). Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance. J Bacteriol 150, 815-825.
14. Howe, M. M. & Margolin, W. (1986). Localization and DNA sequence analysis of the C gene of bacteriophage Mu, the positive regulator of Mu late transcription. Nucleic Acids Res 14, 4881-4897.
15. Itaya, M. & Tanaka, T. (1990). Gene-directed mutagenesis on the chromosome of Bacillus subtilis 168. Mol Gen Genet 223, 268-272.
16. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 277, 680-685.
17. Lopilato, J., Bortner, S. & Beckwith, J. (1986). Mutations in a new chromosomal gene of Escherichia coli, pcnB, reduce plasmid copy number of pBR322 and its derivatives. Mol Gen Genet 205, 285-290.
18. March, P. (1992). Membrane-associated GTPases in bacteria. Mol Microbiol 6, 1253-1257.
19. March, P. E. & Inouye, M. (1985). Characterization of the lep operon of E. coli: identification of the promoter and the gene upstream of the signal peptidase. J Biol Chem 260, 7206-7213.
20. Moran, C. P., Jr, Lang, N., LeGrice, S. F. J., Lee, G., Stephens, M., Sonenshein, A. L., Pero, J. & Losick, R. (1982). Nucleotide sequences that signal initiation of transcription and translation in Bacillus subtilis. Mol Gen Genet 186, 339-346.
21. Saito, H., Shibata, T. & Ando, T. (1979). Mapping of genes determining nonpermissiveness and host-specific restriction to bacteriophages in Bacillus subtilis Marburg. Mol Gen Genet 170, 117-122.
22. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
23. Sanger, F., Nicklen, S. & Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74, 5463-5467.
24. Schulz, A. & Schumann, W. (1996). brcA, the first gene of the Bacillus subtilis dnaK operon encodes a negative regulator of class I heat-shock genes. J Bacteriol 178 1088-1093.
25. Schulz, A., Tzschaschel, B. & Schumann, W. (1995). Isolation and analysis of mutants of the dnaK operon of Bacillus subtilis. Mol Microbiol 15, 421-429.
26. Takemura, K. I., Sato, T. & Kobayashi, Y. (1993). DNA sequence of the lysA-spoIIIC region of the Bacillus subtilis chromosome. EMBL Database, accession no. D17650.
27. Troup, B., Jahn, M., Hungerer, C. & Jahn, D. (1994). Isolation of the hemF operon containing the gene for the Escherichia coli aerobic coproporphyrinogen III oxidase by in vivo complementation of a yeast HEM13 mutant. J Bacteriol 176, 673-680.
28. Troup, B., Hungerer, C. & Jahn, D. (1995). Cloning and characterization of the Escherichia coli hemN gene encoding the oxygen-independent coproporphyrinogen III oxidase. J Bacteriol 177, 3326-3331.
29. Wetzstein, M., Völker, U., Dedio, J., Löbau, S., Zuber, U., Schiesswohl, M., Herget, C., Hecker, M. & Schumann, W. (1992). Cloning, sequencing, and molecular analysis of the dnaK locus from Bacillus subtilis. J Bacteriol 174, 3300-3310.
30. Xu, K. & Elliott, T. (1993). An oxygen-dependent coprophorhyrinogen oxidase encoded by the hemF gene of Salmonella typhimurium. J Bacteriol 175, 4990-4998.
31. Xu, K. & Elliott, T. (1994). Cloning, DNA sequence, and complementation analysis of the Salmonella typhimurium hemN gene encoding a putative oxygen-independent coproporphyrinogen III oxidase. J Bacteriol 176, 3196-3203.
32. Yuan, G. & Wong, S. (1995). Isolation and characterization of Bacillus subtilis regulatory mutants: evidence for orf39 in the dnaK operon as a repressor gene in regulating the expression of both groE and dnaK. J Bacteriol 177, 6462-6468.
33. Zuber, U. & Schumann, W. (1991). Tn5cos: a useful tool for restriction mapping of large plasmids. Gene 103, 69-72.