Establishment of a simple and rapid method to screen for strong promoters in Bacillus subtilis

Protein Expression and Purification

Volumn 71, Issue 2, 2010, Pages 174-178

  Phan, Trang Thi Phuong ^b   Nguyen, Hoang Duc ^b   Schumann, Wolfgang ^a  

^a UNIVERSITY OF BAYREUTH   (Germany)

^b CHEMICAL GENOMICS CENTRE OF THE MAX PLANCK SOCIETY   (Germany)

Author keywords

IPTG; Lac operator; Promoter; UP element

Indexed keywords

5 BROMO 4 CHLORO 3 INDOLYL BETA GALACTOSIDE; 5-BROMO-4-CHLORO-3-INDOLYL BETA-GALACTOSIDE; BETA GALACTOSIDASE; GALACTOSIDE; INDOLE DERIVATIVE; ISOPROPYL THIOGALACTOSIDE;

ARTICLE; BACILLUS SUBTILIS; ESCHERICHIA COLI; GENE VECTOR; GENETICS; HEAT; METABOLISM; REGULATORY SEQUENCE; REPORTER GENE;

BACILLUS SUBTILIS; BETA-GALACTOSIDASE; ESCHERICHIA COLI; GALACTOSIDES; GENES, REPORTER; GENETIC VECTORS; HOT TEMPERATURE; INDOLES; ISOPROPYL THIOGALACTOSIDE; REGULATORY SEQUENCES, NUCLEIC ACID;

BACILLUS SUBTILIS; ESCHERICHIA COLI;

EID: 77949487622     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.11.010     Document Type: Article

References (20)

1. Makrides S.C. Strategies for achieving high-level expression of genes in Escherichia coli. Microbiol. Rev. 60 (1996) 512-538
2. Hannig G., and Makrides S.C. Strategies for optimizing heterologous protein expression in Escherichia coli. Trends Biotechnol. 16 (1998) 54-60
3. Baneyx F. Recombinant protein expression in Escherichia coli. Curr. Opin. Biotechnol. 10 (1999) 411-421
4. Harwood C.R. Bacillus subtilis and its relatives: molecular biological and industrial workhorses. Trends Biotechnol. 10 (1992) 247-256
5. Meima R., Van Dijl J.M., and Bron S. Expression systems in Bacillus. In: Baneyx F. (Ed). Protein Expression Technologies (2004), Horizon Bioscience, Norfold, UK 199-252
6. Schallmey M., Singh A., and Ward O.P. Developments in the use of Bacillus species for industrial production. Can. J. Microbiol. 50 (2004) 1-17
7. Schumann W. Production of recombinant proteins in Bacillus subtilis. Adv. Appl. Microbiol. 62 (2007) 137-189
8. A-dependent promoter sequences: evidence for extended contact between RNA polymerase and upstream promoter DNA. Nucl. Acids Res. 23 (1995) 2351-2360
9. Caramori T., and Galizzi A. The UP element of the promoter for the flagellin gene, hag, stimulates transcription from both SigD- and SigA-dependent promoters in Bacillus subtilis. Mol. Gen. Genet. 258 (1998) 385-388
10. Meijer W.J.J., and Salas M. Relevance of UP elements for three strong Bacillus subtilis phage phi29 promoters. Nucl. Acids Res. 32 (2004) 1166-1176
11. Hirata H., Negoro S., and Okada H. High production of thermostable β-galactosidase of Bacillus stearothermophilus in Bacillus subtilis. Appl. Environ. Microbiol. 49 (1985) 1547-1549
12. Saito H., Shibata T., and Ando T. Mapping of genes determining nonpermissiveness and host-specific restriction to bacteriophages in Bacillus subtilis Marburg. Mol. Gen. Genet. 170 (1979) 117-122
13. Nguyen D.H., Phan T.T.P., and Schumann W. Expression vectors for the rapid purification of recombinant proteins in Bacillus subtilis. Current Microbiol. 55 (2007) 89-93
14. Kim L., Mogk A., and Schumann W. A xylose-inducible Bacillus subtilis integration vector and its application. Gene 181 (1996) 71-76
15. W regulon. Mol. Microbiol. 41 (2001) 59-71
16. W-controlled pbpE gene resulting in filamentous growth of Bacillus subtilis. J. Bacteriol. 185 (2003) 973-982
17. Mogk A., Hayward R., and Schumann W. Integrative vectors for constructing single-copy transcriptional fusions between Bacillus subtilis promoters and various reporter genes encoding heat-stable enzymes. Gene 182 (1996) 33-36
18. Chen I., and Dubnau D. DNA uptake during bacterial transformation. Nat. Rev. Microbiol. 2 (2004) 241-249
19. Phan T.T.P., Nguyen H.D., and Schumann W. Novel plasmid-based expression vectors for intra- and extracellular production of recombinant proteins in Bacillus subtilis. Protein Expr. Purif. 46 (2006) 189-195
20. Titok M.A., Chapuis J., Selezneva Y.V., Lagodich A.V., Prokulevich V.A., Ehrlich S.D., and Jannière L. Bacillus subtilis soil isolates: plasmid replicon analysis and construction of a new theta-replicating vector. Plasmid 49 (2003) 53-62