Expression vectors for the rapid purification of recombinant proteins in Bacillus subtilis

Current Microbiology

Volumn 55, Issue 2, 2007, Pages 89-93

  Nguyen, Hoang Duc ^a   Phan, Trang Thi Phuong ^a   Schumann, Wolfgang ^a  

^a UNIVERSITY OF BAYREUTH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

EPITOPE; MYC PROTEIN;

ARTICLE; BACILLUS SUBTILIS; EXPRESSION VECTOR; GENE OVEREXPRESSION; GENETIC REGULATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SECRETION; REPORTER GENE; SIGNAL TRANSDUCTION;

AMINO ACID SEQUENCE; BACILLUS SUBTILIS; BASE SEQUENCE; EPITOPES; ESCHERICHIA COLI; GENE EXPRESSION; GENE FUSION; GENES, MYC; GENETIC VECTORS; MOLECULAR SEQUENCE DATA; PLASMIDS; RECOMBINANT FUSION PROTEINS;

BACILLUS SUBTILIS;

EID: 34547102994     PISSN: 03438651     EISSN: 14320991     Source Type: Journal    
DOI: 10.1007/s00284-006-0419-5     Document Type: Article

References (25)

1. Anderson RP, Roth JR (1977) Tandem genetic duplications in phage and bacteria. Annu Rev Microbiol 31:473-505
2. Cornet P, Millet J, Béguin P, Aubert JP (1983) Characterization of two cel (cellulose degradation) genes of Clostridium thermocellum coding for endogluanases. Bio/Technology 1:589-594
3. Evan GI, Lewis GK, Ramsay G, Bishop JM (1985) Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol 5:3610-3616
4. Harwood CR (1992) Bacillus subtilis and its relatives: molecular biological and industrial workhorses. Trends Biotechnol 10:247-256
5. Hirata H, Fukazawa T, Negoro S, Okada H (1986) Structure of a β-galactosidase gene of Bacillus stearothermophilus. J Bacteriol 166:722-727
6. Hochuli E (1990) Purification of recombinant proteins with metal chelate adsorbent. Genet Eng (NY) 12:87-98
7. Homuth G, Masuda S, Mogk A, Kobayashi Y, Schumann W (1997) The dnaK operon of Bacillus subtilis is heptacistronic. J Bacteriol. 179:1153-1164
8. Junttila MR, Saarinen S, Schmidt T, Kast J, Westermarck J (2005) Single-step Strep-tag purification for the isolation and identification of protein complexes from mammalian cells. Proteomics 5:1199-1203
9. Kaltwasser M, Wiegert T, Schumann W (2001) Construction and application of epitope- and GFT-tagging integration vectors for Bacillus subtilis. Appl Environ Microbiol 68:2624-2628
10. Kim L, Mogk A, Schumann W (1996) A xylose-inducible Bacillus subtilis integration vector and its application. Gene 181:71-76
11. Meima R, Van Dijl JM, Bron S (2004) Expression systems in Bacillus. In F Baneyx (ed) Protein expression technologies. Horizon Bioscience, Norfold, UK pp 199-252
12. Nguyen DH, Nguyen QA, Ferreira RC, Ferreira LCS, Tran LT, Schumann W. (2004) Construction of plasmid-based expression vectors for Bacillus subtilis. Plasmid 54:241-248
13. Nguyen HD, Schumann W (2006) Establishment of an experimental system allowing immobilization of proteins on the surface of Bacillus subtilis cells. J Biotechnol 122:473-482
14. Pallen MJ, Lam AC, Antonio M, Dunbar K (2001) An embarrassment of sortases: a richness of substrates? Trends Microbiol 9:97-101
15. Palva I (1982) Molecular cloning of alpha-amylase gene from Bacillus amyloliquefaciens and its expression in B. subtilis. Gene 19:81-87
16. Phan TTP, Nguyen HD, Schumann W (2006) Novel plasmid-based expression vectors for intra- and extracellular production of recombinant proteins in Bacillus subtilis. Protein Expr Purif 46:189-195
17. Ripio MT, Dominguez-Bernal G, Suarez M, Brehem K, Berche P, Vazquez-Boland JA (1996) Transcriptional activation of virulence genes in wild-type strains of Listeria monocytogenes in response to a change in the extracellular medium composition. Res Microbiol 147:371-378
18. Saito H, Shibata T, Ando T (1979) Mapping of genes determining nonpermissiveness and host-specific restriction to bacteriophages in Bacillus subtilis Marburg. Mol Gen Genet 170:117-122
19. Schallmey M, Singh A, Ward OP (2004) Developments in the use of Bacillus species for industrial production. Can J Microbiol 50:1-17
20. Schmidt TG, Koepke J, Frank R, Skerra A (1996) Molecular interaction between the Strep-tag affinity peptide and its cognate target, streptavidin. J Mol Biol 255:753-766
21. Schmidt TG, Skerra A (1993) The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment. Protein Eng 6:109-122
22. Scholz Q, Thiel A, Hillen W, Niederweis M (2000) Quantitative analysis of gene expression with an improved green fluorescent protein. Eur J Biochem 267:1565-1570
23. Schulz A, Schwab S, Versteeg S, Schumann W (1997) The htpG gene of Bacillus subtilis belongs to class III heat shock genes and is under negative control. J Bacteriol 10:3103-3109
24. Titok MA, Chapuis J, Selezneva YV, Lagodich AV, Prokulevich VA, Ehrlich SD, Jannière L (2003) Bacillus subtilis soil isolates: plasmid replicon analysis and construction of a new theta-replicating vector. Plasmid 49:53-62
25. Westers L, Westers H, Quax WJ (2004) Bacillus subtilis as cell factory for pharmaceutical proteins: a biotechnological approach to optimize the host organism. Biochim Biophys Acta 1694:299-310