Divergent interactions involving the oxidosqualene cyclase and the steroid-3-ketoreductase in the sterol biosynthetic pathway of mammals and yeasts

Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids

Volumn 1801, Issue 11, 2010, Pages 1232-1237

  Taramino, Silvia ^a   Teske, Brian ^b   Oliaro Bosso, Simonetta ^a   Bard, Martin ^b   Balliano, Gianni ^a  

^a UNIVERSITY OF TURIN   (Italy)

^b INDIANA UNIVERSITY   (United States)

Author keywords

Cholesterol biosynthesis; ERG7; HSD17B7; NS0 cells; Protein protein interaction; Saccharomyces cerevisiae

Indexed keywords

17BETA HYDROXYSTEROID DEHYDROGENASE TYPE 7; LANOSTEROL SYNTHASE; OXIDOREDUCTASE; OXIDOSQUALENE CYCLASE; RECOMBINANT ENZYME; STEROID 3 KETOREDUCTASE; TESTOSTERONE 17BETA DEHYDROGENASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL LINE; CELL LINE NSO; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SUBSTRATE; GROWTH RATE; MAMMAL CELL; MASS SPECTROMETRY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; SACCHAROMYCES CEREVISIAE; STEROL SYNTHESIS; THIN LAYER CHROMATOGRAPHY;

17-HYDROXYSTEROID DEHYDROGENASES; 3-HYDROXYSTEROID DEHYDROGENASES; ANIMALS; CELL LINE, TUMOR; CHOLESTEROL; HUMANS; INTRAMOLECULAR TRANSFERASES; LIPIDS; MICE; OXIDOREDUCTASES; PROTEIN INTERACTION MAPPING; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SPECIES SPECIFICITY; STEROLS;

MAMMALIA; MURINAE; SACCHAROMYCES CEREVISIAE;

EID: 84755160716     PISSN: 13881981     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbalip.2010.07.006     Document Type: Article

References (30)

1. Zhou W.C., Chen C.-C., Buckland B., Aunins J.G. Fed-batch culture of recombinant NS0 myeloma cells with high monoclonal antibody production. Biotechnol. Bioeng. 1997, 55:783-792.
2. Chu L., Robinson D.K. Industrial choice for protein production by large scale cell cultures. Curr. Opin. Biotechnol. 2001, 12:180-187.
3. Seth G., McIvor R.S., Hu W.-S. 17β-Hydroxysteroid dehydrogenase type 7 (Hsd17b7) reverts cholesterol auxotrophy in NS0 cells. J. Biotechnol. 2006, 121:241-252.
4. Seth G., Ozturk M., Hu W.-S. Reverting cholesterol auxotrophy of NS0 cells by altering epigenetic gene silencing. Biotechnol. Bioeng. 2006, 93:820-827.
5. Nokelainen P., Peltoketo H., Vihko R., Vihko P. Expression cloning of a novel estrogenic mouse 17 β-hydroxysteroid dehydrogenase/17-ketosteroid reductase (m17HSD7), previously described as a prolactin receptor-associated protein (PRAP) in rat. Mol. Endocrinol. 1998, 12:1048-1059.
6. Breitling R., Krazeisen A., Möller G., Adamski J. 17b-hydroxysteroid dehydrogenase type 7-an ancient 3-ketosteroid reductase of cholesterogenesis. Mol. Cell. Endocrinol. 2001, 171:199-204.
7. Marijanovic Z., Laubner D., Möller G., Gege C., Hunsen B., Adamski J., Breitling R. Closing the gap: identification of human 3-ketosteroid reductase, the last unknown enzyme of mammalian cholesterol biosynthesis. Mol. Endocrinol. 2003, 17:1715-1725.
8. Shehu A., Mao J., Gibori G.B., Halperin J., Le J., Devi Y.S., Merrill B., Kiyokawa H., Gibori G. Prolactin receptor-associated protein/17β-hydroxysteroid dehydrogenase type 7 gene (Hsd17b7) plays a crucial role in embryonic development and fetal survival. Mol. Endocrinol. 2008, 22:2268-2277.
9. Gachotte D., Sen S.E., Eckstein J., Barbuch R., Krieger M., Ray B.D., Bard M. Characterization of the Saccharomyces cerevisiae ERG27 gene encoding the 3-keto reductase involved in C-4 sterol demethylation. Proc. Natl. Acad. Sci. 1999, 96:12655-12660.
10. Mo C., Milla P., Athenstaedt K., Ott R., Balliano G., Daum G., Bard M. In yeast sterol biosynthesis the 3-ketoreductase protein (Erg27p) is required for oxidosqualene cyclase (Erg7p) activity. Biochim. Biophys. Acta 2003, 1633:68-74.
11. Pierson C.A., Jia N., Mo C., Lees N.D., Strum A.M., Eckstein J., Barbuch R., Bard M. Isolation, characterization, and regulation of the Candida albicans ERG27 gene encoding the sterol 3-keto reductase. Med. Mycol. 2004, 42:461-473.
12. Thoma R., Schulz-Gasch T., D'Arcy B., Benz J., Aebi J., Dehmlow H., Hennig M., Stihle M., Ruf A. Insight into steroid scaffold formation from the structure of human oxidosqualene cyclase. Nature 2004, 432:118-122.
13. Huff M.W., Telford D.E. Lord of the rings-the mechanism for oxidosqualene:lanosterol cyclase becomes crystal clear. Trends Pharmacol. Sci. 2005, 26:335-340.
14. Teske B., Taramino S., Bhuiyan M.S.A., Kumaraswami N.S., Randall S.K., Barbuch R., Eckstein J., Balliano G., Bard M. Genetic analyses involving interactions between the ergosterol biosynthetic enzymes, lanosterol synthase (Erg7p) and 3-ketoreductase (Erg27p), in the yeast Saccharomyces cerevisiae. Biochim. Biophys. Acta 2008, 1781:359-366.
15. Milla P., Athenstaedt K., Viola F., Oliaro-Bosso S., Kohlwein S.D., Daum G., Balliano G. Yeast oxidosqualene cyclase (Erg7p) is a major component of lipid particles. J. Biol. Chem. 2002, 277:2406-2412.
16. Taramino S., Valachovic M., Oliaro-Bosso S., Viola F., Teske B., Bard M., Balliano G. Interactions of oxidosqualene cyclase (Erg7p) with 3-keto reductase (Erg27p) and other enzymes of sterol biosynthesis in yeast. Biochim. Biophys. Acta 2010, 1801:156-162.
17. Mumberg D., Müller R., Funk M. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds. Gene 1995, 156:119-122.
18. Ausubel F.M., Brent R., Kingston R.E., Moore D.D., Seidman J.D., Smith J., Struhl K. Current protocols in molecular biology 1999, Wiley-Interscience, NY.
19. Chan B.E., Knowles B.R. A solvent system for delipidation of plasma or serum without protein precipitation. J. Lipid Res. 1976, 17:176-181.
20. Balliano G., Viola F., Ceruti M., Cattel L. Inhibition of sterol biosynthesis in Saccharomyces cerevisiae by N,N-diethylazasqualene and derivatives. Biochim. Biophys. Acta 1988, 959:9-19.
21. Oliaro-Bosso S., Viola F., Matsuda S.P.T., Cravotto G., Tagliapietra S., Balliano G. Umbelliferone aminoalkyl derivatives as inhibitors of oxidosqualene cyclases from Saccharomyces cerevisiae, Trypanosoma cruzi, and Pneumocystis carinii. Lipids 2004, 39:1007-1012.
22. Peterson G.L. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal. Biochem. 1977, 83:346-356.
23. Gachotte D., Eckstein J., Barbuch R., Hughes T., Roberts C., Bard M. A novel gene conserved from yeast to humans is involved in sterol biosynthesis. J. Lipid Res. 2001, 42:150-154.
24. Morand O.H., Aebi J.D., Dehmlow H., Ji Y.H., Gains N., Lengsfeld H., Himber J. Ro 48-8071, a new 2, 3-oxidosqualene:lanosterol cyclase inhibitor lowering plasma cholesterol in hamsters, squirrel monkeys, and minipigs: comparison to simvastatin. J. Lipid Res. 1997, 38:373-390.
25. Oliaro-Bosso S., Taramino S., Viola F., Tagliapietra S., Ermondi G., Cravotto G., Balliano G. Umbelliferone aminoalkyl derivatives as inhibitors of human oxidosqualene-lanosterol cyclase. J. Enzyme Inhib. Med. Chem. 2009, 24:589-598.
26. Milla P., Viola F., Ceruti M., Rocco F., Cattel L., Balliano G. 19-Azasqualene-2, 3-epoxide and its N-oxide: metabolic fate and inhibitory effect on sterol biosynthesis in Saccharomyces cerevisiae. Lipids 1999, 34:681-688.
27. Hughes A.L., Todd B.L., Espenshade P.J. SREBP pathway responds to sterols and functions as an oxygen sensor in fission yeast. Cell 2005, 120:831-842.
28. Osborne T.F., Espenshade P.J. Evolutionary conservation and adaptation in the mechanism that regulates SREBP action: what a long, strange tRIP it's been. Genes Dev. 2009, 23:2578-2591.
29. Albertini C., Leroux P. A Botrytis cinerea putative 3-keto reductase gene (ERG27) that is homologous to the mammalian 17β-hydroxysteroid dehydrogenase type 7 gene (17β-HSD7). Eur. J. Plant Pathol. 2004, 110:723-733.
30. Fillinger S., Leroux P., Auclair C., Barreau C., Al Hajj C., Debieu D. Genetic analysis of fenhexamid-resistant field isolates of the phytopathogenic fungus Botrytis cinerea. Antimicrob. Agents Chemother. 2008, 52:3933-3940.