A novel gene conserved from yeast to humans is involved in sterol biosynthesis

Journal of Lipid Research

Volumn 42, Issue 1, 2001, Pages 150-154

  Gachotte, D ^a   Eckstein, J ^b   Barbuch, R ^b   Hughes, T ^c   Roberts, C ^c   Bard, M ^a  

^a INDIANA UNIVERSITY   (United States)

^b ELI LILLY AND COMPANY   (United States)

^c ROSETTA INPHARMATICS   (United States)

Author keywords

C 4 demethylation; Ergosterol; Saccharomyces cerevisiae

Indexed keywords

CARBOXYLIC ACID DERIVATIVE; CARBOXYLYASE; DNA; ERGOSTEROL; OXIDOREDUCTASE; STEROL;

AMINO ACID SEQUENCE; ARABIDOPSIS; ARTICLE; BIOSYNTHESIS; DNA SEQUENCE; ENZYME ACTIVITY; GAS CHROMATOGRAPHY; GENETIC TRANSCRIPTION; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; SCHIZOSACCHAROMYCES POMBE; STEROL SYNTHESIS; YEAST;

AMINO ACID SEQUENCE; CONSERVED SEQUENCE; ERGOSTEROL; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; GENE DELETION; GENES, FUNGAL; GENETIC VECTORS; HUMANS; LIPIDS; MALE; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; MULTIENZYME COMPLEXES; PROTEINS; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE ALIGNMENT; STEROLS; YEASTS;

EID: 0035137828     PISSN: 00222275     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Biochemistry, cell biology, and molecular biology of lipids of Saccharomyces cerevisiae
2. Functional discovery via a compendium of expression profiles
3. Functional analysis of the genes of yeast chromosome V by genetic footprinting
4. Cholesterol biosynthesis
5. Cloning and characterization of ERG25, the Saccharomyces gene encoding C-4 sterol methyl oxidase
6. Characterization of the Saccharomyces cerevisiae ERG26 gene encoding the C-3 sterol dehydrogenase (C-4 decarboxylase) involved in sterol biosynthesis
7. Characterization of the Saccharomyces cerevisiae ERG27 gene encoding the 3-keto reductase involved in C-4 sterol demethylation
8. Designer deletion strains from Saccharomyces cerevisiae S288C: A useful set of strains and plasmids for PCR-mediated gene disruption and other applications
9. Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis
10. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae
11. New yeast-Escherichia coli shuttle vectors constructed in vitro mutagenized yeast genes lacking six-base pair restriction sites
12. A rapid method for localized mutagenesis of yeast genes
13. In vitro mutagenesis and plasmid shuffling - From cloned gene to mutant yeast
14. Introduction of plasmid DNA into cells
15. A rapid method of total lipid extraction and purification
16. Polyene resistance and the isolation of sterol mutants in Saccharomyces cerevisiae
17. A novel human gene, encoding a potential membrane protein conserved from yeast to man, is strongly expressed in testis and cancer cell lines
18. A simple method for displaying the hydrophobic character of a protein
19. Signals for retention of transmembrane proteins in the endoplasmic reticulum studied with CD4 truncation mutants
20. Physiological implications of sterol biosynthesis in yeast
21. Cloning and disruption of the yeast C-8 sterol isomerase gene
22. Cloning disruption, and sequence of the gene encoding the yeast C-5 sterol desaturase
23. Identification and characterization of major lipid particle proteins of the yeast Saccharomyces cerevisiae