An in vitro assay for the kinase activity of mTOR complex 2

Methods in Molecular Biology

Volumn 821, Issue , 2012, Pages 75-86

  Huang, Jingxiang ^a  

^a NATIONAL UNIVERSITY HOSPITAL   (Singapore)

Author keywords

Akt; Kinase; mTOR; Rictor; Sin1

Indexed keywords

CRTC2 PROTEIN, HUMAN; TRANSCRIPTION FACTOR;

ARTICLE; AUTOPHAGY; CELL STRAIN HEK293; HELA CELL; HUMAN; IMMUNOPRECIPITATION; METABOLISM; METHODOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS;

AUTOPHAGY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HEK293 CELLS; HELA CELLS; HUMANS; IMMUNOPRECIPITATION; TRANSCRIPTION FACTORS;

EID: 84555189171     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-430-8_6     Document Type: Article

References (27)

1. Wullschleger S, Loewith R, and Hall MN. (2006) TOR signaling in growth and metabolism, Cell 124, 471-484. (Pubitemid 43199434)
2. Sarbassov DD, Guertin DA, Ali SM, and Sabatini DM. (2005) Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex, Science 307, 1098-1101. (Pubitemid 40262113)
3. Guertin DA, Stevens DM, Thoreen CC, Burds AA, Kalaany NY, Moffat J, Brown M, Fitzgerald KJ, and Sabatini DM. (2006) Ablation in mice of the mTORC components raptor, rictor, or mLST8 reveals that mTORC2 is required for signaling to Akt-FOXO and PKCalpha, but not S6K1, Dev Cell 11, 859-871. (Pubitemid 44804279)
4. Sarbassov DD, Ali SM, Kim DH, Guertin DA, Latek RR, Erdjument-Bromage H, Tempst P, and Sabatini DM. (2004) Rictor, a novel binding partner of mTOR, defines a rapamycininsensitive and raptor-independent pathway that regulates the cytoskeleton, Curr Biol 14, 1296-1302. (Pubitemid 38991819)
5. Garcia-Martinez JM, and Alessi DR. (2008) mTOR complex 2 (mTORC2) controls hydrophobic motif phosphorylation and activation of serum- and glucocorticoid-induced protein kinase 1 (SGK1), Biochem J 416, 375-385.
6. Facchinetti V, Ouyang W, Wei H, Soto N, Lazorchak A, Gould C, Lowry C, Newton AC, Mao Y, Miao RQ, Sessa WC, Qin J, Zhang P, Su B, and Jacinto E. (2008) The mammalian target of rapamycin complex 2 controls folding and stability of Akt and protein kinase C, EMBO J 27, 1932-1943.
7. Ikenoue T, Inoki K, Yang Q, Zhou X, and Guan KL. (2008) Essential function of TORC2 in PKC and Akt turn motif phosphorylation, maturation and signalling, EMBO J 27, 1919-1931.
8. Masri J, Bernath A, Martin J, Jo OD, Vartanian R, Funk A, and Gera J. (2007) mTORC2 activity is elevated in gliomas and promotes growth and cell motility via overexpression of rictor, Cancer Res 67, 11712-11720.
9. Guertin DA, Stevens DM, Saitoh M, Kinkel S, Crosby K, Sheen JH, Mullholland DJ, Magnuson MA, Wu H, and Sabatini DM. (2009) mTOR complex 2 is required for the development of prostate cancer induced by Pten loss in mice, Cancer Cell 15, 148-159.
10. Soukas AA, Kane EA, Carr CE, Melo JA, and Ruvkun G. (2009) Rictor/TORC2 regulates fat metabolism, feeding, growth, and life span in Caenorhabditis elegans, Genes Dev 23, 496-511.
11. Jones KT, Greer ER, Pearce D, and Ashrafi K. (2009) Rictor/TORC2 regulates Caenorhabditis elegans fat storage, body size, and development through sgk-1, PLoS Biol 7, e60.
12. Cybulski N, Polak P, Auwerx J, Ruegg MA, and Hall MN. (2009) mTOR complex 2 in adipose tissue negatively controls wholebody growth, Proc Natl Acad Sci USA 106, 9902-9907.
13. Peterson TR, Laplante M, Thoreen CC, Sancak Y, Kang SA, Kuehl WM, Gray NS, and Sabatini DM. (2009) DEPTOR is an mTOR inhibitor frequently overexpressed in multiple myeloma cells and required for their survival, Cell 137, 873-886.
14. Huang J, Dibble CC, Matsuzaki M, and Manning BD. (2008) The TSC1-TSC2 complex is required for proper activation of mTOR complex 2, Mol Cell Biol 28, 4104-4115. (Pubitemid 351812994)
15. Huang J, Wu S, Wu CL, and Manning BD. (2009) Signaling events downstream of mammalian target of rapamycin complex 2 are attenuated in cells and tumors deficient for the tuberous sclerosis complex tumor suppressors, Cancer Res 69, 6107-6114.
16. Dibble CC, Asara JM, and Manning BD. (2009) Characterization of Rictor phosphorylation sites reveals direct regulation of mTOR complex 2 by S6K1, Mol Cell Biol 29, 5657-5670.
17. Julien LA, Carriere A, Moreau J, and Roux PP. (2009) mTORC1-Activated S6K1 Phosphorylates Rictor on Threonine 1135 and Regulates mTORC2 Signaling, Mol Cell Biol 2010; 30, 908-921.
18. Treins C, Warne PH, Magnuson MA, Pende M, and Downward J. (2009) Rictor is a novel target of p70 S6 kinase-1, Oncogene 2010; 29, 1003-1016.
19. Pearce LR, Huang X, Boudeau J, Pawlowski R, Wullschleger S, Deak M, Ibrahim AF, Gourlay R, Magnuson MA, and Alessi DR. (2007) Identification of Protor as a novel Rictorbinding component of mTOR complex-2, Biochem J 405, 513-522. (Pubitemid 47172049)
20. Woo SY, Kim DH, Jun CB, Kim YM, Haar EV, Lee SI, Hegg JW, Bandhakavi S, Griffin TJ, and Kim DH. (2007) PRR5, a novel component of mTOR complex 2, regulates platelet-derived growth factor receptor beta expression and signaling, J Biol Chem 282, 25604-25612. (Pubitemid 47372842)
21. Nowak P, Cole DC, Brooijmans N, Bursavich MG, Curran KJ, Ellingboe JW, Gibbons JJ, Hollander I, Hu Y, Kaplan J, Malwitz DJ, Toral-Barza L, Verheijen JC, Zask A, Zhang WG, and Yu K. (2009) Discovery of potent and selective inhibitors of the mammalian target of rapamycin (mTOR) kinase, J Med Chem 52, 7081-7089.
22. Yu K, Toral-Barza L, Shi C, Zhang WG, Lucas J, Shor B, Kim J, Verheijen J, Curran K, Malwitz DJ, Cole DC, Ellingboe J, Ayral-Kaloustian S, Mansour TS, Gibbons JJ, Abraham RT, Nowak P, and Zask A. (2009) Biochemical, cellular, and in vivo activity of novel ATP-competitive and selective inhibitors of the mammalian target of rapamycin, Cancer Res 69, 6232-6240.
23. Huang J, and Manning BD. (2009) A complex interplay between Akt, TSC2 and the two mTOR complexes, Biochem Soc Trans 37, 217-222.
24. Wullschleger S, Loewith R, Oppliger W, and Hall MN. (2005) Molecular organization of target of rapamycin complex 2, J Biol Chem 280, 30697-30704. (Pubitemid 41291798)
25. Loewith R, Jacinto E, Wullschleger S, Lorberg A, Crespo JL, Bonenfant D, Oppliger W, Jenoe P, and Hall MN. (2002) Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control, Mol Cell 10, 457-468. (Pubitemid 35284167)
26. Yang Q, Inoki K, Ikenoue T, and Guan KL. (2006) Identification of Sin1 as an essential TORC2 component required for complex formation and kinase activity, Genes Dev 20, 2820-2832. (Pubitemid 44771744)
27. Manning BD, Tee AR, Logsdon MN, Blenis J, and Cantley LC. (2002) Identification of the tuberous sclerosis complex-2 tumor suppressor gene product tuberin as a target of the phosphoinositide 3-kinase/akt pathway, Mol Cell 10, 151-162. (Pubitemid 34876569)