Biomaterial surface modifications can dominate cell-substrate mechanics: The impact of PDMS plasma treatment on a quantitative assay of cell stiffness

Soft Matter

Volumn 8, Issue 3, 2012, Pages 673-681

  Bartalena, G ^a,b   Loosli, Y ^a,b,c   Zambelli, T ^b   Snedeker, J G ^a,b  

^a UNIVERSITY OF ZURICH   (Switzerland)

^b ETH ZURICH   (Switzerland)

^c RMS Foundation   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN CYTOSKELETON; AFM; BIOINERT; BIOMATERIAL SURFACES; BULK POLYMER; CELL CULTURE SUBSTRATE; CELL MATRIX; CELL MECHANICS; CELL STIFFNESS; CELL SUBSTRATES; CELL-BIOMATERIAL INTERACTIONS; DECREASING EFFECT; ELASTIC PROPERTIES; EXPERIMENTAL DATA; FINITE ELEMENT MODELS; FINITE ELEMENTS; INDENTATION DEPTH; INDENTATION EXPERIMENT; INDENTERS; MATERIAL MODELS; MECHANICAL CHARACTERIZATIONS; MECHANICAL EFFECTS; MECHANICAL INTERACTIONS; MULTISCALES; PDMS SURFACES; PLASMA TREATMENT; POLYDIMETHYLSILOXANE PDMS; POLYMER SUBSTRATE; QUANTITATIVE ASSAY; QUANTITATIVE ASSESSMENTS; SPHERICAL INDENTATIONS; SUBSTRATE MATERIAL; SURFACE LAYERS; SURFACE MECHANICAL PROPERTIES; SYNTHETIC POLYMERS; THICK LAYERS; TWO LAYERS; UNIVERSAL TESTING MACHINES;

ATOMIC FORCE MICROSCOPY; CELL CULTURE; CELLS; ELASTIC MODULI; FINITE ELEMENT METHOD; MICROCHANNELS; PLASMA APPLICATIONS; PLASMAS; POLYMERS; PROTEINS; SILICONES; SUBSTRATES; SURFACES;

BIOMECHANICS;

EID: 84255197137     PISSN: 1744683X     EISSN: 17446848     Source Type: Journal    
DOI: 10.1039/c1sm06250f     Document Type: Article

References (53)

1. R. Lima et al., Axisymmetric polydimethysiloxane microchannels for in vitro hemodynamic studies Biofabrication 2009 1 3 035005
2. M. C. Liu Y. C. Tai A 3-D microfluidic combinatorial cell array Biomed. Microdevices 2011 13 1 191 201
3. A. Dodge et al., PDMS-based microfluidics for proteomic analysis Analyst 2006 131 10 1122 1128 (Pubitemid 44465542)
4. W. Roos et al., Microtubule gliding and cross-linked microtubule networks on micropillar interfaces Nano Lett. 2005 5 12 2630 2634 (Pubitemid 43088923)
5. T. Steinberg et al., Early keratinocyte differentiation on micropillar interfaces Nano Lett. 2007 7 2 287 294 (Pubitemid 46383585)
6. M. H. Chen Y. J. Chuang F. G. Tseng Self-masked high-aspect-ratio polymer nanopillars Nanotechnology 2008 19 50 505301
7. C. Poulard et al., Mechanical tuning of adhesion through micro-patterning of elastic surfaces Soft Matter 2011 7 6 2543 2551
8. P. J. Wipff et al., The covalent attachment of adhesion molecules to silicone membranes for cell stretching applications Biomaterials 2009 30 9 1781 1789
9. G. Bartalena et al., A novel method for assessing adherent single-cell stiffness in tension: design and testing of a substrate-based live cell functional imaging device Biomed. Microdevices 2011 13 2 291 301
10. Y. W. Lin et al., Understanding sensory nerve mechanotransduction through localized elastomeric matrix control PLoS One 2009 4 1 e4293
11. K. M. Choi J. A. Rogers A photocurable poly(dimethylsiloxane) chemistry designed for soft lithographic molding and printing in the nanometer regime J. Am. Chem. Soc. 2003 125 14 4060 4061 (Pubitemid 36512295)
12. W. Tan T. A. Desai Layer-by-layer microfluidics for biomimetic three-dimensional structures Biomaterials 2004 25 7-8 1355 1364 (Pubitemid 37476212)
13. Y. L. Wang R. J. Pelham, Jr Preparation of a flexible, porous polyacrylamide substrate for mechanical studies of cultured cells, in Methods in Enzymology 1998 489 496
14. J. Roth et al., Surface functionalization of silicone rubber for Permanent adhesion Improvement Langmuir 2008 24 21 12603 12611
15. D. Bodas C. Khan-Malek Formation of more stable hydrophilic surfaces of PDMS by plasma and chemical treatments Microelectron. Eng. 2006 83 4-9 1277 1279
16. S. R. Haines et al., Changes in the electronic structure of silicone rubber surfaces induced by oxygen plasma treatment Surf. Interface Anal. 2007 39 12-13 942 947 (Pubitemid 351153428)
17. K. L. Mills et al., The mechanical properties of a surface-modified layer on poly(dimethylsiloxane) J. Mater. Res. 2008 23 1 37 48 (Pubitemid 351167411)
18. S. Befahy et al., Thickness and elastic modulus of plasma treated PDMS silica-like surface layer Langmuir 2010 26 5 3372 3375
19. D. E. Discher P. Janmey Y. L. Wang Tissue cells feel and respond to the stiffness of their substrate Science 2005 310 5751 1139 1143 (Pubitemid 41681732)
20. A. J. Engler et al., Matrix elasticity Directs stem cell Lineage Specification Cell 2006 126 4 677 689 (Pubitemid 44233625)
21. C. M. Lo et al., Cell movement is guided by the rigidity of the substrate Biophys. J. 2000 79 1 144 152
22. A. Buxboim I. L. Ivanovska D. E. Discher Matrix elasticity, cytoskeletal forces and physics of the nucleus: how deeply do cells 'feel' outside and in? J. Cell Sci. 2010 123 3 297 308
23. A. Buxboim et al., How deeply cells feel: methods for thin gels J. Phys.: Condens. Matter 2010 22 19 194116
24. R. W. Kent et al., Spherical indentation load-relaxation of soft biological tissues J. Mater. Res. 2006 21 8 2003 2010 (Pubitemid 44469136)
25. H. Hillborg et al., Crosslinked polydimethylsiloxane exposed to oxygen plasma studied by neutron reflectometry and other surface specific techniques Polymer 2000 41 18 6851 6863 (Pubitemid 30345375)
26. T. P. Appelman J. Mizrahi D. Seliktar A finite element model of cell-matrix interactions to study the differential effect of scaffold composition on chondrogenic response to mechanical stimulation J. Biomech. Eng. 2011 133 4 041010
27. M. E. Wall et al., Comparison of cellular strain with applied substrate strain in vitro J. Biomech. 2007 40 1 173 181 (Pubitemid 44838686)
28. W. W. Ahmed M. H. Kural T. A. Saif A novel platform for in situ investigation of cells and tissues under mechanical strain Acta Biomater. 2010
29. N. Caille Y. Tardy J. J. Meister Assessment of strain field in endothelial cells subjected to uniaxial deformation of their substrate Ann. Biomed. Eng. 1998 26 3 409 416 (Pubitemid 128609282)
30. C. M. Cheng R. L. Steward, Jr P. R. LeDuc Probing cell structure by controlling the mechanical environment with cell-substrate interactions J. Biomech. 2009 42 2 187 192
31. C. L. Gilchrist et al., Measurement of intracellular strain on deformable substrates with texture correlation J. Biomech. 2007 40 4 786 794 (Pubitemid 46273466)
32. J. P. Butler et al., Traction fields, moments, and strain energy that cells exert on their surroundings Am. J. Physiol. Cell Physiol. 2002 282 3 C595 605
33. B. Sabass et al., High resolution traction force microscopy based on experimental and computational advances Biophys. J. 2008 94 1 207 220
34. U. S. Schwarz et al., Calculation of forces at focal adhesions from elastic substrate data: the effect of localized force and the need for regularization Biophys. J. 2002 83 3 1380 1394 (Pubitemid 34977712)
35. D. Fuard et al., Optimization of poly-di-methyl-siloxane (PDMS) substrates for studying cellular adhesion and motility Microelectron. Eng. 2008 85 5-6 1289 1293
36. T. Tzvetkova-Chevolleau et al., The motility of normal and cancer cells in response to the combined influence of the substrate rigidity and anisotropic microstructure Biomaterials 2008 29 10 1541 1551
37. A. Gouldstone et al., Indentation across size scales and disciplines: Recent developments in experimentation and modeling Acta Mater. 2007 55 12 4015 4039 (Pubitemid 46881292)
38. G. Bar et al., Investigation of the stiffness change in, the indentation force and the hydrophobic recovery of plasma-oxidized polydimethylsiloxane surfaces by tapping mode atomic force microscopy Polymer 2001 42 8 3627 3632 (Pubitemid 32092965)
39. H. J. Donahue et al., Effect of surface nanoscale topography on elastic modulus of individual osteoblastic cells as determined by atomic force microscopy J. Biomech. 2007 40 13 2865 2871 (Pubitemid 47385816)
40. D. Docheva et al., Researching into the cellular shape, volume and elasticity of mesenchymal stem cells, osteoblasts and osteosarcoma cells by atomic force microscopy J. Cell. Mol. Med. 2008 12 2 537 552 (Pubitemid 351555133)
41. I. Firkowska E. Godehardt M. Giersig Interaction between human Osteoblast cells and Inorganic two-dimensional Scaffolds based on Multiwalled Carbon Nanotubes: a quantitative AFM study Adv. Funct. Mater. 2008 18 23 3765 3771
42. L. Lu et al., Mechanical properties of actin stress fibers in living cells Biophys. J. 2008 95 12 6060 6071
43. Y. Loosli R. Luginbuehl J. G. Snedeker Cytoskeleton reorganization of spreading cells on micro-patterned islands: a functional model Philos. Trans. R. Soc., A 2010 368 1920 2629 2652
44. F. Guilak J. R. Tedrow R. Burgkart Viscoelastic properties of the cell nucleus Biochem. Biophys. Res. Commun. 2000 269 3 781 786 (Pubitemid 30440377)
45. C. Dong R. Skalak K. L. Sung Cytoplasmic rheology of passive neutrophils Biorheology 1991 28 6 557 567
46. J. T. Parsons A. R. Horwitz M. A. Schwartz Cell adhesion: integrating cytoskeletal dynamics and cellular tension Nat. Rev. Mol. Cell Biol. 2010 11 9 633 643
47. S. S. Lateef et al., GRGDSP peptide-bound silicone membranes withstand mechanical flexing in vitro and display enhanced fibroblast adhesion Biomaterials 2002 23 15 3159 3168 (Pubitemid 34522129)
48. N. Volcker et al., Functionalization of silicone rubber for the covalent immobilization of fibronectin J. Mater. Sci.: Mater. Med. 2001 12 2 111 119 (Pubitemid 32178860)
49. A. W. Feinberg J. F. Schumacher A. B. Brennan Engineering high-density endothelial cell monolayers on soft substrates Acta Biomater. 2009 5 6 2013 2024
50. M. Dembo Y. L. Wang Stresses at the cell-to-substrate interface during locomotion of fibroblasts Biophys. J. 1999 76 4 2307 2316 (Pubitemid 29266384)
51. J. P. Butler et al., Traction fields, moments, and strain energy that cells exert on their surroundings Am. J. Physiol. Cell Physiol. 2002 282 3 595 605
52. R. I. Sharma J. G. Snedeker Biochemical and biomechanical gradients for directed bone marrow stromal cell differentiation toward tendon and bone Biomaterials 2010 31 30 7695 7704
53. A. Buxboim D. E. Discher Stem cells feel the difference Nat. Methods 2010 7 9 695 697