Stresses at the cell-to-substrate interface during locomotion of fibroblasts

Biophysical Journal

Volumn 76, Issue 4, 1999, Pages 2307-2316

  Dembo, Micah ^a,c   Wang, Yu Li ^b  

^a Boston University   (United States)

^b UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

^c Dept of Biomedical Engineering ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COLLAGEN; POLYACRYLAMIDE;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL FUNCTION; CELL MOTION; CELL STRUCTURE; CELL TRANSPORT; ELASTICITY; FIBROBLAST; LAMELLIPODIUM; MATHEMATICAL ANALYSIS; MICROSCOPY; MOUSE; NONHUMAN; PROTEIN BINDING; STRUCTURE ANALYSIS;

EID: 0033066120     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(99)77386-8     Document Type: Article

References (35)

1. Abercrombie, M., J. E. M. Heaysman, and S. M. Pegrum. 1970a. The locomotion of fibroblasts in culture. I. Movements of the leading edge. Exp. Cell Res. 59:393-398.
2. Abercrombie, M., J. E. M. Heaysman, and S. M. Pegrum. 1970b. The locomotion of fibroblasts in culture. II. Ruffling. Exp. Cell Res. 60: 437-444.
3. Barocas, V. H., and R. T. Tranquillo. 1997. An anisotropic biphasic theory of tissue-equivalent mechanics: the interplay among cell traction, fibrillar network deformation, fibril alignment, and cell contact guidance. J. Biomech. Eng. 119:137-145.
4. Bernardo, J. M., and A. F. M. Smith. 1994. Bayesian Theory. John Wiley and Sons, Chichester, UK.
5. Burton, K., and D. L. Taylor. 1997. Traction forces of cytokinesis measured with optically modified substrata. Nature. 385:450-454.
6. Chen, W. T. 1981. Mechanism of retraction of the trailing edge during fibroblast movement. J. Cell Biol. 90:187-200.
7. Chen, C. S., M. Mrksich, S. Huang, G. M. Whitesides, and D. E. Ingber. 1997. Geometric control of cell life and death. Science. 276:1425-1428.
8. Chrzanowska-Wodnicka, M., and K. Burridge. 1996. Rho-stimulated contractility drives the formation of stress fibers and focal adhesions. J. Cell Biol. 133:1403-1415.
9. Condeelis, J. 1993. Life at the leading edge: the formation of cell protrusions. Annu. Rev. Cell Biol. 9:411-444.
10. Cramer, L. P., and T. J. Mitchison. 1993. Moving and stationary actin filaments are involved in spreading of postmitotic PtK3 cells. J. Cell Biol. 122:833-843.
11. Cramer, L. P., M. Siebert, and T. J. Mitchison. 1997. Identification of novel graded polarity actin filaments in locomoting heart fibroblasts: implications for the generation of motile force. J. Cell Biol. 136: 1287-1305.
12. Dembo, M., T. Oliver, A. Ishihara, and K. Jacobson. 1996. Imaging the traction stresses exerted by locomoting cells with the elastic substratum method. Biophys. J. 70:2008-2022.
13. DiMilla, P. A., K. Barbee, and D. A. Lauffenburger. 1991. Mathematical model for the effects of adhesion and mechanics on cell migration speed. Biophys. J. 60:15-37.
14. Efron, B., and R. Tibshirani. 1986. Bootstrap methods for standard errors, confidence intervals, and other measures of statistical accuracy. Statistical Sci. 1:54-77.
15. Folkman, J., and A. Moscona. 1978. Role of cell shape in growth control. Nature. 273:354-349.
16. Galbraith, C. B., and M. P. Sheetz. 1997. A micromachined device provides a new bend on fibroblast traction forces. Proc. Natl. Acad. Sci. USA. 94:9114-9118.
17. Harris, A. K. 1988. Fibroblasts and myofibroblasts. Methods Enzymol. 163:623-642.
18. Harris, A. K., P. Wild, and D. Stopak. 1980. Silicone rubber substrata: a new wrinkle in the study of cell locomotion. Science. 208:177-179.
19. Landau, L. D., and E. M. Lifshitz. 1986. Theory of Elasticity, 3rd ed, J. B. Sykes and W. H. Reid, translators. Pergamon Press, Oxford, UK.
20. Lauffenburger, D. A., and A. L. Horwitz. 1996. Cell migration: a physically integrated molecular process. Cell. 84:359-369.
21. Lee, J., M. Lenord, T. N. Oliver, A. Ishihara, and K. Jacobson. 1994. Traction forces generated by locomoting cells. J. Cell Biol. 127: 1957-1964.
22. Machesky, L. M., and A. Hall. 1997. Role of actin polymerization and adhesion to extracellular matrix in rac and rho-induced cytoskeletal reorganization. J. Cell Biol. 138:913-926.
23. McKenna, N. M., Y-L. Wang, and M. E. Konkel. 1989. Formation and movement of myosin-containing structures in living fibroblasts. J. Cell Biol. 109:1163-1172.
24. Nobes, C. A., and A. Hall. 1995. Rho, rac, and Cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia. Cell. 81:53-62.
25. Oliver, T., M. Dembo, and K. Jacobson. 1995. Traction forces in locomoting cells. Cell Motil. Cytoskeleton. 31:225-240.
26. Oliver, T., K. Jacobson, and M. Dembo. 1998. Design and use of substrata to measure traction forces exerted by cultured cells. Methods Enzymol. 298:497-488.
27. Oliver, T., J. Lee, and K. Jacobson. 1994. Forces exerted by locomoting cells. Seminars Cell Biol 5:139-147.
28. Pelham, R. J., and Y-L. Wang. 1997. Cell locomotion and focal adhesions are regulated by substrate flexibility. Proc. Natl. Acad. Sci. USA. 94: 13661-13665.
29. Schmidt, C. E., A. F. Horwitz, D. A. Lauffenburger, and M. P. Sheetz. 1993. Integrin-cytoskeletal interactions in migrating fibroblasts are dynamic, asymmetric, and regulated. J. Cell Biol. 123:977-991.
30. Sheetz, M. P. 1994. Cell migration by graded attachment to substrates and contraction. Seminars Cell Biol. 5:149-155.
31. Sheetz, M. P., D. P. Felsenfeld, and C. G. Galbraith. 1998, Cell migration: regulation of force on extracellular matrix-integrin complexes. Trends Cell Biol. 8:51-54.
32. Small, J. V., K. Rottner, I. Kaverina, and K. I. Anderson. 1998. Assembling an actin cytoskeleton for cell attachment and movement. Biochim. Biophys. Acta., in press.
33. Verkhovsky, A. B., and G. G. Borisy. 1993. Non-sarcomeric mode of myosin II organization in fibroblast lamellum. J. Cell Biol. 123: 637-652.
34. Wang, Y-L. 1985. Exchange of actin subunits at the leading edge of living fibroblasts: possible role of treadmilling. J. Cell Biol. 101:597-602.
35. Wang, Y-L., and R. J. Pelham, Jr. 1998. Preparation of a flexible, porous polyacrylamide substrate for mechanical studies of cultured cells. Methods Enzymol. 298:489-496.