How deeply cells feel: Methods for thin gels

Journal of Physics Condensed Matter

Volumn 22, Issue 19, 2010, Pages

  Buxboim, Amnon ^a   Rajagopal, Karthikan ^a   Brown, Andre E X ^a,b   Discher, Dennis E ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CELL RESPONSE; CELL SENSITIVITY; EFFECTIVE STIFFNESS; GEL SURFACE; MATRIX; MESENCHYMAL STEM CELL; MICROENVIRONMENTS; MICRON SCALE; POLYACRYLAMIDE HYDROGELS; POLYMER CONCENTRATIONS; RIGID OBJECTS; RIGID SUBSTRATES; TISSUE CELLS; VERY SOFT TISSUE; VINYL GROUP;

ATOMIC FORCE MICROSCOPY; CELL CULTURE; CONFOCAL MICROSCOPY; ELASTICITY; GLASS; POLYMERIZATION; POLYMERS; STEM CELLS; STIFFNESS; STRUCTURAL DESIGN;

GELS;

ACRYLIC ACID RESIN; BIOMIMETIC MATERIAL; POLYACRYLAMIDE GELS;

ANIMAL; ARTICLE; CELL ADHESION; CHEMISTRY; FOCAL ADHESION; MECHANICAL STRESS; MECHANOTRANSDUCTION; MESENCHYMAL STEM CELL; MOUSE; PHYSIOLOGY; SHEAR STRENGTH; SURFACE PROPERTY; VISCOSITY; YOUNG MODULUS;

ACRYLIC RESINS; ANIMALS; BIOMIMETIC MATERIALS; CELL ADHESION; ELASTIC MODULUS; FOCAL ADHESIONS; MECHANOTRANSDUCTION, CELLULAR; MESENCHYMAL STEM CELLS; MICE; SHEAR STRENGTH; STRESS, MECHANICAL; SURFACE PROPERTIES; VISCOSITY;

EID: 77951567666     PISSN: 09538984     EISSN: 1361648X     Source Type: Journal    
DOI: 10.1088/0953-8984/22/19/194116     Document Type: Article

References (36)

1. Flanagan L A, Ju Y E, Marg B, Osterfield M and Janmey P A 2002 Neurite branching on deformable substrates Neuroreport 13 2411-5
2. Georges P C, Miller W J, Meaney D F, Sawyer E S and Janmey P A 2006 Matrices with compliance comparable to that of brain tissue select neuronal over glial growth in mixed cortical cultures Biophys. J. 90 3012-8
3. Patel P N, Smith C K and Patrick C W 2005 Rheological and recovery properties of poly(ethylene glycol) diacrylate hydrogels and human adipose tissue J. Biomed. Mater. Res. A 73A 313-9
4. Ferrari G et al 1998 Muscle regeneration by bone marrow derived myogenic progenitors Science 279 1528-30
5. Engler A J, Griffin M A, Sen S, Bonnetnann C G, Sweeney H L and Discher D E 2004 Myotubes differentiate optimally on substrates with tissue-like stiffness: pathological implications for soft or stiff microenvironments J. Cell Biol. 166 877-87
6. Stolz M, Raiteri R, Daniels A U, VanLandingham M R, Baschong W and Aebi U 2004 Dynamic elastic modulus of porcine articular cartilage determined at two different levels of tissue organization by indentation-type atomic force microscopy Biophys. J. 86 3269-83
7. Andrades J A, Santamaria J A, Nimni M E and Becerra J 2001 Selection and amplification of a bone marrow cell population and its induction to the chondro-osteogenic lineage by rhOP-1: an in vitro and in vivo study Int. J. Dev. Biol. 45 689-93
8. Engler A J, Sen S, Sweeney H L and Discher D E 2006 Matrix elasticity directs stem cell lineage specification Cell 126 677-89
9. Holmbeck K et al 1999 MT1-MMP-deficient mice develop dwarfism, osteopenia, arthritis, and connective tissue disease due to inadequate collagen turnover Cell 99 81-92
10. Morinobu M et al 2003 Osteopontin expression in osteoblasts and osteocytes during bone formation under mechanical stress in the calvarial suture in vivo J. Bone Miner. Res. 18 1706-15
11. Discher D E, Janmey P and Wang Y L 2005 Tissue cells feel and respond to the stiffness of their substrate Science 310 1139-43
12. Pelham R J and Wang Y L 1997 Cell locomotion and focal adhesions are regulated by substrate flexibility Proc. Natl Acad. Sci. USA 94 13661-5
13. Solon J, Levental I, Sengupta K, Georges P C and Janmey P A 2007 Fibroblast adaptation and stiffness matching to soft elastic substrates Biophys. J. 93 4453-61
14. Guilak F, Alexopoulos L G, Haider M A, Ting-Beall H P and Setton L A 2005 Zonal uniformity in mechanical properties of the chondrocyte pericellular matrix: micropipette aspiration of canine chondrons isolated by cartilage homogenization Ann. Biomed. Eng. 33 1312-8
15. Sodek J and McKee M D 2000 Molecular and cellular biology of alveolar bone Periodontology 24 99-126
16. Poole C A, Flint M H and Beaumont B W 1987 Chondrons in cartilage-ultrastructural analysis of the pericellular microenvironment in adult human articular cartilages J. Orthop. Res. 5 509-22
17. Gaudet C et al 2003 Influence of type I collagen surface density on fibroblast spreading, motility, and contractility Biophys. J. 85 3329-35
18. Engler A, Bacakova L, Newman C, Hategan A, Griffin M and Discher D 2004 Substrate compliance versus ligand density in cell on gel responses Biophys. J. 86 617-28
19. Rowlands A S, George P A and Cooper-White J J 2008 Directing osteogenic and myogenic differentiation of MSCs: interplay of stiffness and adhesive ligand presentation Am. J. Physiol. Cell Physiol. 295 C1037-44
20. Buxboim A, Ivanovska I L and Discher D E 2010 Matrix elasticity, cytoskeletal forces and physics of the nucleus: how deeply do cells 'feel' outside and in? J. Cell Sci. 123 297-308
21. Harris A K, Wild P and Stopak D 1980 Silicone-rubber substrata-new wrinkle in the study of cell locomotion Science 208 177-9
22. Cesa C M, Kirchgessner N, Mayer D, Schwarz U S, Hoffmann B and Merkel R 2007 Micropatterned silicone elastomer substrates for high resolution analysis of cellular force patterns Rev. Sci. Instrum. 78 034301
23. Li Y, Hu Z B and Li C F 1993 New method for measuring Poisson ratio in polymer gels J. Appl. Polym. Sci. 50 1107-11
24. Boudou T, Ohayon J, Arntz Y, Finet G, Picart C and Tracqui P 2006 An extended modeling of the micropipette aspiration experiment for the characterization of the Young's modulus and Poisson's ratio of adherent thin biological samples: numerical and experimental studies J. Biomech. 39 1677-85
25. Beatty F M and Stalnaker D O 1986 The Poisson function of finite elasticity J. Appl. Mech. 53 807-13
26. Brown A E X, Litvinov R I, Discher D E, Purohit P K and Weisel J W 2009 Multiscale mechanics of fibrin polymer: gel stretching with protein unfolding and loss of water Science 325 741-4
27. Kondo T, Johnson S A, Yoder M C, Romand R and Hashino E 2005 Sonic hedgehog and retinoic acid synergistically promote sensory fate specification from bone marrow-derived pluripotent stem cells Proc. Natl Acad. Sci. USA 102 4789-94
28. Engler A J, Richert L, Wong J Y, Picart C and Discher D E 2004 Surface probe measurements of the elasticity of sectioned tissue, thin gels and polyelectrolyte multilayer films: correlations between substrate stiffness and cell adhesion Surf. Sci. 570 142-54
29. Hertz H 1882 ber die Berührung fester elastischer Körper J. Reine Angew. Math. 92 156-71
30. Domke J and Radmacher M 1998 Measuring the elastic properties of thin polymer films with the atomic force microscope Langmuir 14 3320-5
31. Sen S, Engler A J and Discher D E 2009 Matrix strains induced by cells: computing how far cells can fell Cell. Mol. Bioeng. 2 39-48
32. Balaban N Q et al 2001 Force and focal adhesion assembly: a close relationship studied using elastic micropatterned substrates Nat. Cell Biol. 3 466-72
33. Merkel R, Kirchgebner N, Cesa C M and Hoffmann B 2007 Cell force microscopy on elastic layers of finite thickness Biophys. J. 93 3314-23
34. Maloney J M, Walton E B, Bruce C M and Vliet K J 2008 Influence of finite thickness and stiffness on cellular adhesion-induced deformation of compliant substrata Phys. Rev. E 78 041923
35. Yue Z Q 1996 On elastostatics of multilayered solids subjected to general surface traction Q. J. Mech. Appl. Math. 49 471-99
36. Winer J P, Oake S and Janmey P A 2009 Non-linear elasticity of extracellular matrices enables contractile cells to communicate local position and orientation Plos One 4 e6382