A novel multiplex PCR/RFLP assay for the identification of Streptococcus bovis/Streptococcus equinus complex members from dairy microbial communities based on the 16S rRNA gene

FEMS Microbiology Letters

Volumn 326, Issue 2, 2012, Pages 144-150

  Jans, Christoph ^a   Lacroix, Christophe ^a   Meile, Leo ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

Dairy fermentation; Dairy streptococci; PCR RFLP identification assay; SBSEC; Streptococcus gallolyticus; Streptococcus infantarius

Indexed keywords

RNA 16S;

ARTICLE; BACTERIAL GENE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; CONTROLLED STUDY; GENE IDENTIFICATION; GENE SEQUENCE; GENE TARGETING; GENETIC CONSERVATION; MICROBIAL COMMUNITY; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SEQUENCE ANALYSIS; SPECIES DIFFERENCE; SPECIES IDENTIFICATION; STREPTOCOCCUS; STREPTOCOCCUS ALACTOLYTICUS; STREPTOCOCCUS BOVIS; STREPTOCOCCUS EQUINUS; STREPTOCOCCUS GALLOLYTICUS;

BACTERIOLOGICAL TECHNIQUES; FOOD MICROBIOLOGY; GENES, RRNA; MOLECULAR DIAGNOSTIC TECHNIQUES; MULTIPLEX POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY; STREPTOCOCCUS;

LACTOCOCCUS; STREPTOCOCCUS; STREPTOCOCCUS ALACTOLYTICUS; STREPTOCOCCUS EQUINUS; STREPTOCOCCUS GALLOLYTICUS; STREPTOCOCCUS INFANTARIUS;

EID: 83855160745     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2011.02443.x     Document Type: Article

References (32)

1. Abdelgadir W, Nielsen DS, Hamad S & Jakobsen M (2008) A traditional Sudanese fermented camel's milk product, Gariss, as a habitat of Streptococcus infantarius subsp. infantarius. Int J Food Microbiol 127: 215-219.
2. Beck M, Frodl R & Funke G (2008) Comprehensive study of strains previously designated Streptococcus bovis consecutively isolated from human blood cultures and emended description of Streptococcus gallolyticus and Streptococcus infantarius subsp. coli. J Clin Microbiol 46: 2966-2972.
3. Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J & Sayers EW (2009) GenBank. Nucleic Acids Res 37: D26-D31.
4. Chen H-J, Tsai J-C, Chang T-C, Hung W-C, Tseng S-P, Hsueh P-R & Teng L-J (2008) PCR-RFLP assay for species and subspecies differentiation of the Streptococcus bovis group based on groESL sequences. J Med Microbiol 57: 432-438.
5. Clarridge JE (2004) Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clin Microbiol Rev 17: 840-862.
6. Delbès C, Ali-Mandjee L & Montel M-C (2007) Monitoring bacterial communities in raw milk and cheese by culture-dependent and -independent 16S rRNA gene-based analyses. Appl Environ Microbiol 73: 1882-1891.
7. Díaz-Ruiz G, Guyot JP, Ruiz-Teran F, Morlon-Guyot J & Wacher C (2003) Microbial and physiological characterization of weakly amylolytic but fast-growing lactic acid bacteria: a functional role in supporting microbial diversity in pozol, a Mexican fermented maize beverage. Appl Environ Microbiol 69: 4367-4374.
8. Franciosi E, Settanni L, Cavazza A & Poznanski E (2009) Biodiversity and technological potential of wild lactic acid bacteria from raw cows' milk. Int Dairy J 19: 3-11.
9. Gevers D, Huys G & Swings J (2001) Applicability of rep-PCR fingerprinting for identification of Lactobacillus species. FEMS Microbiol Lett 205: 31-36.
10. Giannino ML, Marzotto M, Dellaglio F & Feligini M (2009) Study of microbial diversity in raw milk and fresh curd used for Fontina cheese production by culture-independent methods. Int J Food Microbiol 130: 188-195.
11. Glazunova OO, Raoult D & Roux V (2009) Partial sequence comparison of the rpoB, sodA, groEL and gyrB genes within the genus Streptococcus. Int J Syst Evol Microbiol 59: 2317-2322.
12. Goldenberger D, Perschil I, Ritzler M & Altwegg M (1995) A simple universal DNA extraction procedure using SDS and proteinase K is compatible with direct PCR amplification. PCR Methods Appl 4: 368-370.
13. Grice EA, Kong HH, Renaud G, NISC Comparative Sequencing Program, Young AC, Bouffard GG, Blakesley RW, Wolfsberg TG, Turner ML & Segre JA (2008) A diversity profile of the human skin microbiota. Genome Res 18: 1043-1050.
14. Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41: 95-98.
15. Hinse D, Vollmer T, Erhard M, Welker M, Moore ERB, Kleesiek K & Dreier J (2011) Differentiation of species of the Streptococcus bovis/equinus-complex by MALDI-TOF Mass Spectrometry in comparison to sodA sequence analyses. Syst Appl Microbiol 34: 52-57.
16. Hols P, Hancy F, Fontaine L et al. (2005) New insights in the molecular biology and physiology of Streptococcus thermophilus revealed by comparative genomics. FEMS Microbiol Rev 29: 435-463.
17. Jain R, Rivera MC & Lake JA (1999) Horizontal gene transfer among genomes: the complexity hypothesis. P Natl Acad Sci USA 96: 3801-3806.
18. Jans C (2011) Biodiversity of lactic acid bacteria in raw camel milk products of East Africa including genomic and functional characterization of predominant lactose-adapted Streptococcus infantarius subsp. infantarius. PhD Thesis No. 19835, ETH Zurich, Zurich.
19. Liu ZZ, DeSantis TZ, Andersen GL & Knight R (2008) Accurate taxonomy assignments from 16S rRNA sequences produced by highly parallel pyrosequencers. Nucleic Acids Res 36: e120.
20. Makarova K, Slesarev A, Wolf Y et al. (2006) Comparative genomics of the lactic acid bacteria. P Natl Acad Sci USA 103: 15611-15616.
21. Pacini F, Cariolato D, Andrighetto C & Lombardi A (2006) Occurrence of Streptococcus macedonicus in Italian cheeses. FEMS Microbiol Lett 261: 69-73.
22. Papadelli M, Manolopoulou E, Kalantzopoulos G & Tsakalidou E (2003) Rapid detection and identification of Streptococcus macedonicus by species-specific PCR and DNA hybridisation. Int J Food Microbiol 81: 231-239.
23. Poyart C, Quesne G, Coulon S, Berche P & Trieu-Cuot P (1998) Identification of streptococci to species level by sequencing the gene encoding the manganese-dependent superoxide dismutase. J Clin Microbiol 36: 41-47.
24. Poyart C, Quesne G & Trieu-Cuot P (2002) Taxonomic dissection of the Streptococcus bovis group by analysis of manganese-dependent superoxide dismutase gene (sodA) sequences: reclassification of 'Streptococcus infantarius subsp. coli' as Streptococcus lutetiensis sp. nov. and of Streptococcus bovis biotype II.2 as Streptococcus pasteurianus sp. nov. Int J Syst Evol Microbiol 52: 1247-1255.
25. Rashid MHU, Togo K, Ueda M & Miyamoto T (2009) Characterization of bacteriocin produced by Streptococcus bovis J2 40-2 isolated from traditional fermented milk 'Dahi'. Anim Sci J 80: 70-78.
26. Renye JA Jr, Somkuti GA, Van Hekken DL & Guerrero Prieto VM (2011) Short communication: characterization of microflora in Mexican Chihuahua cheese. J Dairy Sci 94: 3311-3315.
27. Schlegel L, Grimont F, Ageron E, Grimont PAD & Bouvet A (2003) Reappraisal of the taxonomy of the Streptococcus bovis/Streptococcus equinus complex and related species: description of Streptococcus gallolyticus subsp. gallolyticus subsp. nov., S. gallolyticus subsp. macedonicus subsp. nov. and S. gallolyticus subsp. pasteurianus subsp. nov. Int J Syst Evol Microbiol 53: 631-645.
28. Tsakalidou E, Zoidou E, Pot B, Wassill L, Ludwig W, Devriese LA, Kalantzopoulos G, Schleifer KH & Kersters K (1998) Identification of streptococci from Greek Kasseri cheese and description of Streptococcus macedonicus sp. nov. Int J Syst Bacteriol 48: 519-527.
29. Whiley RA & Hardie JM (2009) Genus I. Streptococcus. Bergey's Manual of Systematic Bacteriology. The Firmicutes, Vol. 3 (De Vos P, Garrity GM, Jones D, Krieg NR, Ludwig W, Rainey FA, Schleifer KH & Whitman WB, eds), pp. 655-711. Springer, New York, NY.
30. Wullschleger S (2009) Biodiversity and microbial safety of artisanal Malian sour milk fènè and development of adapted starter cultures for controlled production. PhD Thesis No. 18287, ETH Zurich, Zurich.
31. Yarza P, Richter M, Peplies J, Euzeby J, Amann R, Schleifer KH, Ludwig W, Glöckner FO & Rosselló-Móra R (2008) The all-species living tree project: a 16S rRNA-based phylogenetic tree of all sequenced type strains. Syst Appl Microbiol 31: 241-250.
32. Younan M & Bornstein S (2007) Lancefield group B and C streptococci in East African camels (Camelus dromedarius). Vet Rec 160: 330-335.