Mapping nanomechanical properties of live cells using multi-harmonic atomic force microscopy

Nature Nanotechnology

Volumn 6, Issue 12, 2011, Pages 809-814

  Raman, A ^a   Trigueros, S ^b   Cartagena, A ^a   Stevenson, A P Z ^b   Susilo, M ^a   Nauman, E ^a,c   Contera, S Antoranz ^b  

^a PURDUE UNIVERSITY   (United States)

^b UNIVERSITY OF OXFORD   (United Kingdom)

^c PURDUE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADHESION; CELL CULTURE; CELL MEMBRANES; DRUG DELIVERY; ESCHERICHIA COLI; STIFFNESS;

DYNAMIC ATOMIC FORCE MICROSCOPY; ESCHERICHIA COLI BACTERIA; HARMONIC COMPONENTS; HUMAN RED BLOOD CELL; MECHANOTRANSDUCTION; NANOMECHANICAL PROPERTY; SPATIAL AND TEMPORAL RESOLUTIONS; VISCOELASTIC DISSIPATION;

ATOMIC FORCE MICROSCOPY;

ARTICLE; ATOMIC FORCE MICROSCOPY; BIOFILM; CELL SURFACE; CONTROLLED STUDY; ERYTHROCYTE; ESCHERICHIA COLI; FIBROBLAST; FOURIER ANALYSIS; HUMAN; MOLECULAR MECHANICS; NONHUMAN; PRIORITY JOURNAL; RAT; TUMOR; VISCOELASTICITY;

EID: 83555162569     PISSN: 17483387     EISSN: 17483395     Source Type: Journal    
DOI: 10.1038/nnano.2011.186     Document Type: Article

References (34)

1. Nelson, C. M. et al. Emergent patterns of growth controlled by multi-cellular form and mechanics. Proc. Natl Acad. Sci USA 102, 11597 (2005).
2. Ingber, D. E. Tensegrity: the architectural basis of cellular mechanotransduction. Annu. Rev. Physiol. 59, 575-599 (1997). (Pubitemid 27142456)
3. Tan, J. L. et al. Cells lying on a bed of microneedles: an approach to isolate mechanical force. Proc. Natl Acad. Sci. USA 100, 1484-1489 (2003). (Pubitemid 36254472)
4. Dembo, M. & Wang, Y. L. Stresses at the cell-to-substrate interface during locomotion of fibroblasts. Biophys. J. 76, 2307-2316 (1999). (Pubitemid 29266384)
5. DuRoure, O. et al. Force mapping in epithelial cell migration. Proc. Natl Acad. Sci. USA 102, 2390-2395 (2005).
6. Suresh, S. Biomechanics and biophysics of cancer cells. Acta Biomater. 3, 413-438 (2007).
7. Palm, K., Luthman, K., Ungell, A. L., Strandlund, G. & Artursson, P. Correlation of drug absorption with molecular surface properties. J. Pharm. Sci. 85, 32-39 (1996). (Pubitemid 26029068)
8. Breukink, E. et al. Use of the cell wall precursor lipid II by a pore-forming peptide antibiotic. Science 286, 2361-2364 (1999). (Pubitemid 30016902)
9. Fantner, G. E., Barbero, R. J., Gray, D. S. & Belcher, A. M. Kinetics of antimicrobial peptide activity measured on individual bacterial cells using high-speed atomic force microscopy. Nature Nanotech. 5, 280-285 (2010).
10. Rotsch, C. & Radmacher, M. Drug-induced changes of cytoskeletal structure and mechanics in fibroblasts: an atomic force microscopy study. Biophys. J. 78, 520-535 (2000). (Pubitemid 30207160)
11. Van Vliet, K. J., Bao, G. & Suresh, S. The biomechanics toolbox: experimental approaches to living cells and biomolecules. Acta Mater. 51, 5881-5905 (2003).
12. Cross, S. E., Jin, Y. S., Rao, J. & Gimzewski, J. K. Nanomechanical analysis of cells from cancer patients. Nature Nanotech. 2, 780-783 (2007). (Pubitemid 350223349)
13. Iyer, S., Gaikwad, R. M., Subba-Rao, V.,Woodworth, C. D. & Sokolov, I. Atomic force microscopy detects differences in the surface brush of normal and cancerous cells. Nature Nanotech. 4, 389-393 (2009).
14. Radmacher, M., Fritz, M., Kacher, C. M., Cleveland, J. P. & Hansma, P. K. Measuring the viscoelastic properties of human platelets with the atomic force microscope. Biophys. J. 70, 556-567 (1996). (Pubitemid 26021495)
15. Matzke, R., Jacobson, K. & Radmacher, M. Direct, high-resolution measurement of furrow stiffening during division of adherent cells. Nature Cell Biol. 3, 607-610 (2001). (Pubitemid 32536859)
16. Arnoldi, M. et al. Bacterial turgor pressure can be measured by atomic force microscopy. Phys. Rev. E 62, 1034-1044 (2000).
17. Hassan, E. A. et al. Relative microelastic mapping of living cells by atomic force microscopy. Biophys. J. 74, 1564-1578 (1998).
18. Melcher, J. et al. Origins of phase contrast in the atomic force microscope in liquids. Proc. Natl Acad. Sci. USA 106, 13655-13660 (2009).
19. Van Noort, S. J. T., Willemsen, O. H., van der Werf, K. O., de Grooth, B. G. & Greve, J. Mapping electrostatic forces using higher harmonics tapping mode atomic force microscopy in liquid. Langmuir 15, 7101 (1999).
20. Preiner, J., Tang, J. L., Patsushenko, V. & Hinterdorfer, P. Higher harmonic atomic force microscopy: imaging of biological membranes in liquid. Phys. Rev. Lett. 99, 046102 (2007).
21. Dulebo, A. et al. Second harmonic atomic force microscopy imaging of live and fixed mammalian cells. Ultramicroscopy 109, 1056 (2009).
22. Turner, R. D., Kirkham, J., Devine, D. & Thomson, N. H. Second harmonic atomic force microscopy of living Staphylococcus aureus bacteria. Appl. Phys. Lett. 94, 043901 (2009).
23. Xu, X., Melcher, J., Reifenberger, R. & Raman, A. Compositional contrast of soft biological materials in liquids using the momentary excitation of higher eigenmodes. Phys. Rev. Lett. 102, 060801 (2009).
24. Mart?́nez, N. F. et al. Bimodal atomic force microscopy imaging of isolated antibodies in air and liquids. Nanotechnology 19, 384011 (2008).
25. Platz, D., Tholen, E. A., Pesen, D. & Haviland, D. B. Intermodulation atomic force microscopy. Appl. Phys. Lett. 92, 153106 (2008).
26. Tetard, L., Passian, A. & Thundat, T. New modes for sub-surface atomic force microscopy through nanomechanical coupling. Nature Nanotech. 5, 105-109 (2010).
27. Tetard, L. et al. Imaging nanoparticles in cells by nanomechanical holography. Nature Nanotech. 3, 501-505 (2008).
28. Dong, M. D., Husale, S. & Sahin, O. Determination of protein structural flexibility by microsecond force spectroscopy. Nature Nanotech. 4, 514-517 (2009).
29. Vadillo-Rodriguez, V. & Dutcher, J. R. Dynamic viscoelastic behavior of individual gram negative bacterial cells. Soft Matter 5, 5012-5019 (2009).
30. San Paulo, A. & Garc?́a, R. Tip sample forces, amplitude and energy dissipation, in amplitude modulation (tapping mode) force microscopy. Phys. Rev. B 64, 193411 (2001).
31. Plodinec, M. et al. The nanomechanical properties of rat fibroblasts are modulated by interfering with the vimentin intermediate filament system. J. Struct. Biol. 174, 476-484 (2011).
32. Alsteen, D. et al. Structure, cell wall elasticity and polysaccharide properties of living yeast cells, as probed by AFM. Nanotechnology 19, 384005 (2008).
33. Dupres, V. et al. Nanoscale mapping and functional analysis of individual adhesins on living bacteria. Nature Methods 2, 515-521 (2005).
34. Heinz, W. F. & Hoh, J. H. Spatially resolved force spectroscopy of biological surfaces using the atomic force microscope. Trends Biotechnol. 7799, 143-150 (1999). (Pubitemid 29273944)