Parallel production and verification of protein products using a novel high-throughput screening method

Biotechnology Journal

Volumn 6, Issue 8, 2011, Pages 1018-1025

  Tegel, Hanna ^a   Yderland, Louise ^a   Boström, Tove ^a   Eriksson, Cecilia ^a   Ukkonen, Kaisa ^b   Vasala, Antti ^b   Neubauer, Peter ^b   Ottosson, Jenny ^a   Hober, Sophia ^a  

^a ALBANOVA UNIVERSITY CENTER   (Sweden)

^b UNIVERSITY OF OULU   (Finland)

Author keywords

His tag; IMAC; Micro scale; Protein production; Protein purification; Screening

Indexed keywords

HIS-TAG; IMAC; MICRO-SCALES; PROTEIN PRODUCTION; PROTEIN PURIFICATION;

AFFINITY CHROMATOGRAPHY; DESORPTION; PURIFICATION; THROUGHPUT;

PROTEINS;

RECOMBINANT PROTEIN;

ARTICLE; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION;

CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; PROTEIN BIOSYNTHESIS; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 79960647620     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201000430     Document Type: Article

References (26)

1. Gräslund, S., Sagemark, J., Berglund, H., Dahlgren, L. G. et al., The use of systematic N- and C-terminal deletions to promote production and structural studies of recombinant proteins. Protein Expr. Purif. 2008, 58, 210-221.
2. Tegel, H., Steen, J., Konrad, A., Nikdin, H. et al., High-throughput protein production - lessons from scaling up from 10 to 288 recombinant proteins per week. Biotechnol. J. 2009, 4, 51-57.
3. Savitsky, P., Bray, J., Cooper, C. D., Marsden, B. D. et al., High-throughput production of human proteins for crystallization: The SGC experience. J. Struct. Biol. 2010, 172, 3-13.
4. Brodsky, O., Cronin, C. N., Economical parallel protein expression screening and scale-up in Escherichia coli. J. Struct. Funct. Genomics 2006, 7, 101-108.
5. Mack, M., Burger, M., Pietschmann, P., Hock, B., A high-throughput mictrotiter plate-based screening method for detection of full-length recombinant proteins. Protein Expr. Purif. 2008, 61, 92-98.
6. Berrow, N. S., Büssow, K., Coutard, B., Diprose, J. et al., Recombinant protein expression and solubility screening in Eschirichia coli: A comparative study. Acta Cryst. 2006, D62, 1218-1226.
7. Uhlén, M., Björling, E., Agaton, C., Szigyarto, C. A. et al., A human protein atlas for normal and cancer tissues based on antibody proteomics. Mol. Cell. Proteomics 2005, 4, 1920-1932.
8. Panula-Perälä, J., Siurkus, J., Vasala, A., Wilmanowski, R. et al., Enzyme controlled glucose auto-delivery for high cell density cultivations in microplates and shake flasks. Microb. Cell Fact. 2008, 7, 31.
9. Krause, M., Ukkonen, K., Haataja, T., Ruottinen, M. et al., A novel fed-batch based cultivation method provides high cell-density and improves yield of soluble recombinant proteins in shaken cultures. Microb. Cell Fact. 2010, 9, 11.
10. Steen, J., Uhlén, M., Hober, S., Ottosson, J., High-throughput protein purification using an automated set-up for high-yield affinity chromatography. Protein Expr. Purif. 2006, 46, 173-178.
11. Larsson, M., Gräslund, S., Yuan, L., Brundell, E. et al., High-throughput protein expression of cDNA products as a tool in functional genomics. J. Biotechnol. 2000, 80, 143-157.
12. Gräslund, S., Nordlund, P., Weigelt, J., Hallberg, B. M. et al., Protein production and purification. Nat. Methods 2008, 5, 135-146.
13. Šiurkus, J., Panula-Perälä, J., Horn, U., Kraft, M. et al., Novel approach of high cell density recombinant bioprocess development: Optimization and scale-up from microlitre to pilot scales while maintaining the fed-batch cultivation mode of E. coli cultures. Microb. Cell Fact. 2010, 9, 35.
14. Taskila, S., Neubauer, P., Tuomola, M., Breitenstein, A. et al., Improved enrichment cultivation of beer spoiling lactic acid bacteria by continuous glucose addition to the culture. J. Inst. Brew. 2009, 115, 177-182.
15. Jeude, M., Dittrich, B., Niederschulte, H., Anderlei, T. et al., Fed-batch mode in shake flaks by slow-release technique. Biotechnol. Bioeng. 2006, 95, 434-445.
16. Page, R., Moy, K., Sims, E. C., Velasquez, J. et al., Scalable high-throughput micro-expression device for recombinant proteins. BioTechniques 2004, 37, 364-370.
17. Huber, R., Ritter, D., Hering, T., Hillmer, A-K. et al., Robo-Lector - a novel platform for automated high-thorughput cultivations in microtiter plates with high information content. Microb. Cell Fact. 2009, 8, 42.
18. Vasala, A., Panula, J., Bollok, M., Illmann, L. et al., A new wireless system for decentralised measurement of physiological parameters from shake flasks. Microb. Cell Fact. 2006, 5, 8.
19. Sørensen, H. P., Mortensen, K. K., Advanced genetic strategies for recombinant protein expression in Escherichia coli. J. Biotechnol. 2005, 115, 113-128.
20. Perkins, J. R., Smith, B., Gallagher, R. T., Jones, D. S. et al., Application of electrospray mass spectrometry and matrix-assisted laser desorption ionization time-of-flight mass spectrometry for molecular weight assignment of peptides in complex mixtures. J. Am. Soc. Mass Spectrom. 1993, 4, 670-684.
21. Hochuli, E., Bannwarth, W., Döbeli, H., Gentz, R. et al., Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent. Nat. Biotechnol. 1988, 6, 1321-1325.
22. Scheich, C., Sievert, V., Büssow, K., An automated method for high-throughput protein purification applied to a comparison of His-tag and GST-tag affinity chromatography. BMC Biotechnol. 2003, 3, 12.
23. Sugar, F. J., Jenney, F. E., Poole, F. L., Brereton, P. S. et al., Comparison of small- and large-scale expression of Pyrococcus furiosus genes as an aid to high-throughput protein production. J. Struct. Funct. Genomics 2005, 6, 149-158.
24. Knaust, R. K., Nordlund, P., Screening for soluble expression of recombinant proteins in a 96-well format. Anal. Biochem. 2001, 297, 79.
25. Nguyen, H., Martinez, B., Oganesyan, N., Kim, R., An automated small-scale protein expression and purification screening provides beneficial information for protein production. J. Struct. Funct. Genomics 2004, 5, 23-27.
26. Schäfer, F., Römer, U., Emmerlich, M., Blümer, J. et al., Automated high-throughput purification of 6xHis-tagged proteins. J. Biomol. Tech. 2002, 13, 131-142.