Essential role of the adaptor protein Nck1 in Jurkat T cell activation and function

Clinical and Experimental Immunology

Volumn 167, Issue 1, 2012, Pages 99-107

  Yiemwattana, I ^a   Ngoenkam, J ^a   Paensuwan, P ^a   Kriangkrai, R ^a   Chuenjitkuntaworn, B ^a   Pongcharoen, S ^a  

^a NARESUAN UNIVERSITY   (Thailand)

Author keywords

CD3; Jurkat T cells; Nck1; SiRNA; T cell activation

Indexed keywords

CD28 ANTIGEN; CD3 ANTIGEN; CD38 ANTIGEN; CD69 ANTIGEN; INTERLEUKIN 2; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; NCK PROTEIN; PHORBOL 13 ACETATE 12 MYRISTATE; PHYTOHEMAGGLUTININ; PROTEIN NCK1; SMALL INTERFERING RNA; T LYMPHOCYTE RECEPTOR; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; CD4+ T LYMPHOCYTE; CELL FUNCTION; CELL PROLIFERATION; CELL STIMULATION; CONTROLLED STUDY; ELECTROPORATION; GENE SILENCING; GENE TARGETING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; LEUKEMIA CELL LINE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; PROTEIN SECRETION; T LYMPHOCYTE ACTIVATION;

ADAPTIVE IMMUNITY; ADAPTOR PROTEINS, SIGNAL TRANSDUCING; ANTIBODIES, MONOCLONAL; ANTIGENS, CD; ANTIGENS, CD28; ANTIGENS, DIFFERENTIATION, T-LYMPHOCYTE; APOPTOSIS; CD4-POSITIVE T-LYMPHOCYTES; ELECTROPORATION; HUMANS; INTERLEUKIN-1; JURKAT CELLS; LECTINS, C-TYPE; LYMPHOCYTE ACTIVATION; MITOGEN-ACTIVATED PROTEIN KINASE 1; MITOGEN-ACTIVATED PROTEIN KINASE 3; ONCOGENE PROTEINS; PHOSPHORYLATION; PHYTOHEMAGGLUTININS; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECEPTOR-CD3 COMPLEX, ANTIGEN, T-CELL; RNA INTERFERENCE; RNA, SMALL INTERFERING; TETRADECANOYLPHORBOL ACETATE;

EID: 82655173757     PISSN: 00099104     EISSN: 13652249     Source Type: Journal    
DOI: 10.1111/j.1365-2249.2011.04494.x     Document Type: Article

References (38)

1. Lehmann JM, Riethmuller G, Johnson JP. Nck, a melanoma cDNA encoding a cytoplasmic protein consisting of the src homology units SH2 and SH3. Nucleic Acids Res 1990; 18:1048.
2. Park D. Cloning, sequencing, and overexpression of SH2/SH3 adaptor protein Nck from mouse thymus. Mol Cells 1997; 7:231-36.
3. Bladt F, Aippersbach E, Gelkop S etal. The murine Nck SH2/SH3 adaptors are important for the development of mesoderm-derived embryonic structures and for regulating the cellular actin network. Mol Cell Biol 2003; 23:4586-597.
4. Chen M, She H, Kim A, Woodley DT, Li W. Nckbeta adapter regulates actin polymerization in NIH 3T3 fibroblasts in response to platelet-derived growth factor bb. Mol Cell Biol 2000; 20:7867-880.
5. Guan S, Chen M, Woodley D, Li W. Nckbeta adapter controls neuritogenesis by maintaining the cellular paxillin level. Mol Cell Biol 2007; 27:6001-11.
6. Pramatarova A, Ochalski PG, Chen K etal. Nck beta interacts with tyrosine-phosphorylated disabled 1 and redistributes in Reelin-stimulated neurons. Mol Cell Biol 2007; 23:7210-221.
7. Lettau M, Pieper J, Janssen O. Nck adapter proteins: functional versatility in T cells. Cell Commun Signal 2009; 7:1.
8. Bunnell SC, Hong DI, Kardon JR etal. T cell receptor ligation induces the formation of dynamically regulated signaling assemblies. J Cell Biol 2002; 158:1263-75.
9. Rivero-Lezcano OM, Marcilla A, Sameshima JH, Robbins KC. Wiskott-Aldrich syndrome protein physically associates with Nck through Src homology 3 domains. Mol Cell Biol 1995; 15:5725-31.
10. Buday L, Wunderlich L, Tamas P. The Nck family of adapter proteins of actin cytoskeleton. Cell Signal 2002; 14:723-31.
11. Alberola-Ila J, Takaki S, Kerner JD, Perlmutter RM. Differential signaling by lymphocyte antigen receptors. Annu Rev Immunol 1997; 15:125-54.
12. Bubeck Wardenburg J, Pappu R, Bu JY etal. Regulation of PAK activation and the T cell cytoskeleton by the linker protein SLP-76. Immunity 1998; 9:607-16.
13. Galisteo ML, Chernoff J, Su YC, Skolnik EY, Schlessinger J. The adaptor protein Nck links receptor tyrosine kinases with the serine-threonine kinase Pak1. J Biol Chem 1996; 271:20997-1000.
14. Lin J, Weiss A. T cell receptor signaling. J Cell Sci 2001; 114:243-44.
15. Gil D, Schamel WW, Montoya M, Sanchez-Madrid F, Alarcon B. Recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. Cell 2002; 109:901-12.
16. Gil D, Schrum AG, Alarcon B, Palmer E. T cell receptor engagement by peptide-MHC ligands induces a conformational change in the CD3 complex of thymocytes. J Exp Med 2005; 201:517-22.
17. Roy E, Togbe D, Holdorf AD etal. Nck adaptors are positive regulators of the size and sensitivity of the T-cell repertoire. Proc Natl Acad Sci USA 2010; 107:15529-34.
18. Szymczak AL, Workman CJ, Gil D etal. The CD3epsilon proline-rich sequence, and its interaction with Nck, is not required for T cell development and function. J Immunol 2005; 175:270-75.
19. Takeuchi K, Yang H, Ng E etal. Structural and functional evidence that Nck interaction with CD3epsilon regulates T-cell receptor activity. J Mol Biol 2008; 380:704-16.
20. Tailor P, Tsai S, Shameli A etal. The proline-rich sequence of CD3ε as an amplifier of low-avidity TCR signaling. J Immunol 2008; 181:243-55.
21. Sawasdikosol S. Detecting tyrosine-phosphorylated proteins by Western blot analysis. Curr Protoc Immunol 2010; 89:11.3.1-11.
22. Sasagawa M, Cech NB, Gray DE, Elmer GW, Wenner CA. Echinacea alkylamides inhibit interleukin-2 production by Jurkat T cells. Int Immunopharmacol 2006; 6:1214-21.
23. Lettau M, Qian J, Linkermann A etal. The adaptor protein Nck interacts with Fas ligand: guiding the death factor to the cytotoxic immunological synapse. Proc Natl Acad Sci USA 2006; 103:5911-16.
24. Kremer BE, Adang LA, Macara IG. Septins regulate actin organization and cell-cycle arrest through nuclear accumulation of NCK mediated by SOCS7. Cell 2007; 130:837-50.
25. Barda-Saad M, Braiman A, Titerence R, Bunnell SC, Barr VA, Samelson LE. Dynamic molecular interactions linking the T cell antigen receptor to the actin cytoskeleton. Nat Immunol 2005; 6:80-9.
26. Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 2001; 411:494-8.
27. Hammond SM, Bernstein E, Beach D, Hannon GJ. An RN. A-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells. Nature 2000; 404:293-6.
28. Zamore PD, Tuschl T, Sharp PA, Bartel DP. RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals. Cell 2000; 101:25-33.
29. Kim JA, Cho K, Shin MS etal. A novel electroporation method using a capillary and wire-type electrode. Biosens Bioelectron 2008; 23:1353-60.
30. Shiow LR, Rosen DB, Brdickova N etal. CD69 acts downstream of interferon-α/β to inhibit S1P1 and lymphocyte egress from lymphoid organs. Nature 2006; 440:540-4.
31. Finco TS, Kadlecek T, Zhang W, Samelson LE, Weiss A. LAT is required for TCR-mediated activation of PLCgamma1 and the Ras pathway. Immunity 1998; 9:617-26.
32. Hu Q, Milfay D, Williams LT. Binding of NCK to SOS and activation of ras-dependent gene expression. Mol Cell Biol 1995; 15:1169-74.
33. Okada S, Pessin JE. Interaction between Src homology (SH) 2/SH3 adaptor proteins and the guanylnucleotide exchange factor SOS are differently regulated by insulin and epidermal growth factor. J Biol Chem 1996; 271:25533-8.
34. Roy E, Togbe D, Holdorf A etal. Fine tuning of the threshold of T cell selection by the Nck adaptors. J Immunol 2010; 185:7518-26.
35. Smith-Gavin JE, Koretzky GA, Jordan MS. T cell activation. Annu Rev Immunol 2009; 27:591-619.
36. Badour K, Zhang J, Siminovitch KA. Involvement of the Wiskott-Aldrich syndrome protein and other actin regulatory adaptors in T cell activation. Semin Immunol 2004; 16:395-407.
37. Snapper SB, Rosen FS, Mizoguchi E etal. Wiskott-Aldrich syndrome protein-deficient mice reveal a role for WASP in T but not B cell activation. Immunity 1998; 9:81-91.
38. Zhang J, Shehabeldin A, da Cruz LAG etal. Antigen receptor-induced activation and cytoskeletal rearrangement are impaired in Wiskott-Aldrich syndrome protein-deficient lymphocytes. J Exp Med 1999; 190:1329-42.