In vitro affinity maturation of an anti-PSA antibody for prostate cancer diagnostic assay

Journal of Molecular Biology

Volumn 414, Issue 4, 2011, Pages 545-562

  Muller, Bruno H ^a   Savatier, Alexandra ^a   L'Hostis, Guillaume ^a   Costa, Narciso ^a   Bossus, Marc ^a   Michel, Sandrine ^b   Ott, Catherine ^c   Becquart, Laurence ^c   Ruffion, Alain ^d,e   Stura, Enrico A ^f   Ducancel, Frédéric ^a  

^a Institut National de la Recherche Agronomique   (France)

^b BIOMÉRIEUX   (France)

^c HOSPICES CIVILS DE LYON   (France)

^d CENTRE HOSPITALIER LYON SUD   (France)

^e UNIVERSITÉ LYON 1   (France)

^f Institute Frederic Joliot   (France)

Author keywords

affinity engineering; hot spots; phage display; prostate specific antigen; scFv antibody fragment

Indexed keywords

DNA; IMMUNOGLOBULIN F(AB) FRAGMENT; PROSTATE SPECIFIC ANTIGEN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIBODY AFFINITY; ARTICLE; COMPLEMENTARITY DETERMINING REGION; COMPLEX FORMATION; CRYSTAL STRUCTURE; DIAGNOSTIC ACCURACY; DIFFERENTIAL DIAGNOSIS; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE MUTATION; HUMAN; IMMUNOASSAY; IN VITRO STUDY; MAJOR CLINICAL STUDY; MALE; MOLECULAR CLONING; MUTAGENESIS; PHAGE DISPLAY; PRIORITY JOURNAL; PROSTATE CANCER; PROSTATE DISEASE; PROSTATE HYPERTROPHY; SANDWICH IMMUNOASSAY; SERUM; STRUCTURE ANALYSIS; X RAY CRYSTALLOGRAPHY;

EID: 82555168293     PISSN: 00222836     EISSN: 10898638     Source Type: Journal    
DOI: 10.1016/j.jmb.2011.10.008     Document Type: Article

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