A gene-fusion strategy for stoichiometric and co-localized expression of light-gated membrane proteins

Nature Methods

Volumn 8, Issue 12, 2011, Pages 1083-1091

  Kleinlogel, Sonja ^a   Terpitz, Ulrich ^a,d   Legrum, Barbara ^a   Gökbuget, Deniz ^a,d,e   Boyden, Edward S ^b   Bamann, Christian ^a   Wood, Phillip G ^a   Bamberg, Ernst ^a,c  

^a MAX PLANCK INSTITUTE OF BIOPHYSICS   (Germany)

^b MIT MEDIA LAB   (United States)

^c GOETHE UNIVERSITY   (Germany)

^d UNIVERSITY OF WÜRZBURG   (Germany)

^e ETH ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIORHODOPSIN; HALORHODOPSIN; LIGHT GATED MEMBRANE PROTEIN; MEMBRANE PROTEIN; RHODOPSIN; UNCLASSIFIED DRUG;

ACCURACY; ANIMAL CELL; ARTICLE; CELL MEMBRANE POTENTIAL; CELL STRAIN HEK293; CHLAMYDOMONAS REINHARDTII; CONTROLLED STUDY; EMBRYO; GENE EXPRESSION; GENE FUSION; GENETIC ENGINEERING; HUMAN; HUMAN CELL; NERVE CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN METABOLISM; RAT; STOICHIOMETRY;

ARTIFICIAL GENE FUSION; BACTERIORHODOPSINS; HEK293 CELLS; HIPPOCAMPUS; HUMANS; LIGHT; MOLECULAR SEQUENCE DATA; NEURONS; RHODOPSIN;

VOLVOX CARTERI;

EID: 82355175134     PISSN: 15487091     EISSN: 15491676     Source Type: Journal    
DOI: 10.1038/nmeth.1766     Document Type: Article

References (34)

1. Nagel, G. et al. Light activation of channelrhodopsin-2 in excitable cells of Caenorhabditis elegans triggers rapid behavioral responses. Curr. Biol. 15, 2279-2284 (2005). (Pubitemid 41790623)
2. Han, X. & Boyden, E. Multiple-color optical activation, silencing, and desynchronization of neural activity, with single-spike temporal resolution. PLoS ONE 2, e299 (2007).
3. Zhang, F. et al. Multimodal fast optical interrogation of neural circuitry. Nature 446, 633-639 (2007). (Pubitemid 46606891)
4. Douin, V. et al. Use and comparison of different internal ribosomal entry sites (IRES) in tricistronic retroviral vectors. BMC Biotechnol. 4, 16 (2004).
5. Han, X., Qian, X., Stern, P., Chuong, A. & Boyden, E. Informational lesions: optical perturbation of spike timing and neural synchrony via microbial opsin gene fusions. Front. Mol. Neurosci. 2, 12 (2009).
6. Tang, W. et al. Faithful expression of multiple proteins via 2A-peptide self-processing: a versatile and reliable method for manipulating brain circuits. J. Neurosci. 29, 8621-8629 (2009).
7. de Felipe, P. et al. E unum pluribus: multiple proteins from a self-processing polyprotein. Trends Biotechnol. 24, 68-75 (2006). (Pubitemid 43151978)
8. Gradinaru, V. et al. Molecular and cellular approaches for diversifying and extending optogenetics. Cell 141, 154-165 (2009).
9. Geibel, S. et al. The voltage-dependent proton pumping in bacteriorhodopsin is characterized by optoelectic behavior. Biophys. J. 81, 2059-2068 (2001). (Pubitemid 32917156)
10. Seki, A. et al. Heterologous expression of Pharaonis halorhodopsin in Xenopus laevis oocytes and electrophysiological characterization of its light-driven Cl pump activity. Biophys. J. 92, 2559-2569 (2007). (Pubitemid 46536437)
11. Nagel, G., Möckel, B., Büldt, G. & Bamberg, E. Functional expression of bacteriorhodopsin in oocytes allows direct measurement of voltage dependence of light induced H+ pumping. FEBS Lett. 377, 263-266 (1995). (Pubitemid 26015079)
12. Shcherbo, D. et al. Bright far-red fluorescent protein for whole-body imaging. Nat. Methods 4, 741-746 (2007). (Pubitemid 47338169)
13. Feldbauer, K. et al. Channelrhodopsin-2 is a leaky proton pump. Proc. Natl. Acad. Sci. USA 106, 12317-12322 (2009).
14. Zufferey, R. et al. Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery. J. Virol. 72, 9873-9880 (1998). (Pubitemid 28520853)
15. Bamann, C., Gueta, R., Kleinlogel, S., Nagel, G. & Bamberg, E. Structural guidance of the photocycle of channelrhodopsin-2 by an interhelical hydrogen bond. Biochemistry 49, 267-278 (2010).
16. Berndt, A., Yizhar, O., Gunaydin, L., Hegemann, P. & Deisseroth, K. Bi-stable neural state switches. Nat. Neurosci. 12, 229-234 (2009).
17. Zhang, F. et al. Red-shifted optogenetic excitation: a tool for fast neural control derived from Volvox carteri. Nat. Neurosci. 11, 631-633 (2008). (Pubitemid 351748117)
18. Chizhov, I. & Engelhard, M. Temperature and halide dependence of the photocycle of halorhodopsin from Natronobacterium pharaonis. Biophys. J. 81, 1600-1612 (2001). (Pubitemid 32783600)
19. Lanyi, J. Halorhodopsin: A light-driven chloride ion pump. Annu. Rev. Biophys. Biophys. Chem. 15, 11-28 (1986).
20. Dencher, N. & Wilms, M. Flash photometric experiments on the photochemical cycle of bacteriorhodopsin. Biophys. Struct. Mech. 1, 259-271 (1975).
21. Chow, B. et al. High-performance genetically targetable optical neural silencing by light-driven proton pumps. Nature 463, 98-102 (2010).
22. 2+-permeable channelrhodopsin CatCh. Nat. Neurosci. 14, 513-518 (2011).
23. Yang, F., Moss, L. & Phillips, G. The molecular structure of green fluorescent protein. Nat. Biotechnol. 14, 1246-1251 (1996). (Pubitemid 26332784)
24. Patterson, G. & Lippincott-Schwartz, J. A photoactivatable GFP for selective photolabeling of proteins and cells. Science 297, 1873-1877 (2002). (Pubitemid 35024347)
25. Nagel, G. et al. Channelrhodopsin-2, a directly light-gated cation-selective membrane channel. Proc. Natl. Acad. Sci. USA 100, 13940-13945 (2003). (Pubitemid 37499170)
26. Hackmann, C. et al. Static and time-resolved step-scan Fourier transform infrared investigations of the photoreaction of halorhodopsin from Natronobacterium pharaonis: consequences for models of the anion translocation mechanism. Biophys. J. 81, 394-406 (2001). (Pubitemid 32605979)
27. Hegemann, P., Oesterhelt, D. & Bamberg, E. The transport activity of the light-driven chloride pump halorhodopsin is regulated by green and blue light. Biochim. Biophys. Acta 819, 195-205 (1985). (Pubitemid 16238672)
28. Pletnev, S. et al. A crystallographic study of bright far-red fluorescent protein mKate reveals pH-induced cis-trans isomerization of the chromophore. J. Biol. Chem. 283, 28980-28987 (2008).
29. Allocca, M. et al. Serotype-dependent packaging of large genes in adeno-associated viral vectors results in effecive gene delivery in mice. J. Clin. Invest. 118, 1955-1964 (2008). (Pubitemid 351632397)
30. Büning, H. et al. Receptor targeting of adeno-associated virus vectors. Gene Ther. 10, 1142-1151 (2003). (Pubitemid 36896589)
31. Allocca, M. et al. Novel adeno-associated virus serotypes efficiently transduce murine photoreceptors. J. Virol. 81, 11372-11380 (2007). (Pubitemid 47536108)
32. Arenkiel, B. et al. In vivo light-induced activation of neural circuitry in transgenic mice expressing channelrhodopsin-2. Neuron 54, 205 (2007).
33. Thyagarajan, S. et al. Visual function in mice with photoreceptor degeneration and transgenic expression of channelrhodopsin 2 in ganglion cells. J. Neurosci. 30, 8745 (2010).
34. Zimmermann, D. et al. Effects on capacitance by overexpression of membrane proteins. Biochem. Biophys. Res. Commun. 369, 1022-1026 (2008).