Heterologous expression of pharaonis halorhodopsin in Xenopus laevis oocytes and electrophysiological characterization of its light-driven Cl - pump activity

Biophysical Journal

Volumn 92, Issue 7, 2007, Pages 2559-2569

  Seki, Akiteru ^a   Miyauchi, Seiji ^a   Hayashi, Saori ^a   Kikukawa, Takashi ^a   Kubo, Megumi ^a   Demura, Makoto ^a   Ganapathy, Vadivel ^b   Kamo, Naoki ^a  

^a HOKKAIDO UNIVERSITY   (Japan)

^b MEDICAL COLLEGE OF GEORGIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANION; BROMINE; CHLORIDE; HALORHODOPSIN; IODINE; NITRATE;

ANION TRANSPORT; ARTICLE; ELECTRODE; ELECTROPHYSIOLOGY; LIGHT INTENSITY; NONHUMAN; OOCYTE; PHOTON; PROTEIN EXPRESSION; STEADY STATE; VOLTAGE CLAMP; XENOPUS LAEVIS;

ARCHAEA; NATRONOMONAS PHARAONIS; XENOPUS LAEVIS;

EID: 34047203574     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1529/biophysj.106.093153     Document Type: Article

References (34)

1. Schobert, B., and J. K. Lanyi. 1982. Halorhodopsin is a light-driven chloride pump. J. Biol. Chem. 257:10306-10313.
2. Mukohata, Y., and Y. Kaji. 1981. Light-induced membrane-potential increase, ATP synthesis, and proton uptake in Halobacterium halobium, R1mR catalyzed by halorhodopsin: effects of N,N′-dicyclohexylcarbodiimide, triphenyltin chloride, and 3,5-di-tert-butyl-4-hydroxybenzylidenemalononitrile (SF6847). Arch. Biochem. Biophys. 206:72-76.
3. Lanyi, J. K. 1990. Halorhodopsin, a light-driven electrogenic chloride-transport system. Physiol. Rev. 70:319-330.
4. Varo, G. 2000. Analogies between halorhodopsin and bacteriorhodopsin. Biochim. Biophys. Acta. 1460:220-229.
5. Duschl, A., J. K. Lanyi, and L. Zimanyi. 1990. Properties and photochemistry of a halorhodopsin from the haloalkalophile, Natronobacterium pharaonis. J. Biol. Chem. 265:1261-1267.
6. Rudiger, M., and D. Oesterhelt. 1997. Specific arginine and threonine residues control anion binding and transport in the light-driven chloride pump halorhodopsin. EMBO J. 16:3813-3821.
7. Lanyi, J. K., A. Duschl, G. W. Hatfield, K. May, and D. Oesterhelt. 1990. The primary structure of a halorhodopsin from Natronobacterium pharaonis. Structural, functional and evolutionary implications for bacterial rhodopsins and halorhodopsins. J. Biol. Chem. 265:1253-1260.
8. Varo, G., L. Zimanyi, X. Fan, L. Sun, R. Needleman, and J. K. Lanyi. 1995. Photocycle of halorhodopsin from Halobacterium salinarium. Biophys. J. 68:2062-2072.
9. Varo, G., L. S. Brown, J. Sasaki, H. Kandori, A. Maeda, R. Needleman, and J. K. Lanyi. 1995. Light-driven chloride ion transport by halorhodopsin from Natronobacterium pharaonis. 1. The photochemical cycle. Biochemistry. 34:14490-14499.
10. Varo, G., R. Needleman, and J. K. Lanyi. 1995. Light-driven chloride ion transport by halorhodopsin from Natronobacterium pharaonis. 2. Chloride release and uptake, protein conformation change, and thermodynamics. Biochemistry. 34:14500-14507.
11. Chizhov, I., and M. Engelhard. 2001. Temperature and halide dependence of the photocycle of halorhodopsin from Natronobacterium pharaonis. Biophys. J. 81:1600-1612.
12. Kolbe, M., H. Besir, L. O. Essen, and D. Oesterhelt. 2000. Structure of the light-driven chloride pump halorhodopsin at 1.8 Å resolution. Science. 288:1390-1396.
13. Essen, L. O. 2002. Halorhodopsin: light-driven ion pumping made simple? Curr. Opin. Struct. Biol. 12:516-522.
14. Sato, M., M. Kubo, T. Aizawa, N. Kamo, T. Kikukawa, K. Nitta, and M. Demura. 2005. Role of putative anion-binding sites in cytoplasmic and extracellular channels of Natronomonas pharaonis halorhodopsin. Biochemistry. 44:4775-4784.
15. Bamberg, E., P. Hegemann, and D. Oesterhelt. 1984. Reconstitution of halorhodopsin in black lipid membranes. Prog. Clin. Biol. Res. 164:73-79.
16. Kalaidzidis, I. V., Y. L. Kalaidzidis, and A. D. Kaulen. 1998. Flash-induced voltage changes in halorhodopsin from Natronobacterium pharaonis. FEBS Lett. 427:59-63.
17. Okuno, D., M. Asaumi, and E. Muneyuki. 1999. Chloride concentration dependency of the electrogenic activity of halorhodopsin. Biochemistry. 38:5422-5429.
18. Muneyuki, E., C. Shibazaki, H. Ohtani, D. Okuno, M. Asaumi, and T. Mogi. 1999. Time-resolved measurements of photovoltage generation by bacteriorhodopsin and halorhodopsin adsorbed on a thin polymer film. J. Biochem. (Tokyo). 125:270-276.
19. Ludmann, K., G. Ibron, J. K. Lanyi, and G. Varo. 2000. Charge motions during the photocycle of pharaonis halorhodopsin. Biophys. J. 78:959-966.
20. Manor, D., C. A. Hasselbacher, and J. L. Spudich. 1988. Membrane potential modulates photocycling rates of bacterial rhodopsins. Biochemistry. 27:5843-5848.
21. Sato, M., T. Kanamori, N. Kamo, M. Demura, and K. Nitta. 2002. Stopped-flow analysis on anion binding to blue-form halorhodopsin from Natronobacterium pharaonis: comparison with the anion-uptake process during the photocycle. Biochemistry. 41:2452-2458.
22. Miyauchi, S., E. Gopal, Y. J. Fei, and V. Ganapathy. 2004. Functional identification of SLC5A8, a tumor suppressor down-regulated in colon cancer, as a Na(1)-coupled transporter for short-chain fatty acids. J. Biol. Chem. 279:13293-13296.
23. Gopal, E., Y. J. Fei, M. Sugawara, S. Miyauchi, L. Zhuang, P. Martin, S. B. Smith, P. D. Prasad, and V. Ganapathy. 2004. Expression of slc5a8 in kidney and its role in Na(1)-coupled transport of lactate. J. Biol. Chem. 279:44522-44532.
24. Scharf, B., and M. Engelhard. 1994. Blue halorhodopsin from Natronobacterium pharaonis: wavelength regulation by anions. Biochemistry. 33:6387-6393.
25. Balint, Z., M. Lakatos, C. Ganea, J. K. Lanyi, and G. Varo. 2004. The nitrate transporting photochemical reaction cycle of the pharaonis halorhodopsin. Biophys. J. 86:1655-1663.
26. Magyari, K., V. Simon, and G. Varo. 2006. The influence of the halide ions on the photochemical reaction cycle of pharaonis halorhodopsin. J. Photochem. Photobiol. B. 82:16-20.
27. Schobert, B., and J. K. Lanyi. 1986. Electrostatic interaction between anions bound to site I and the retinal Schiff base of halorhodopsin. Biochemistry. 25:4163-4167.
28. Shibata, M., N. Muneda, T. Sasaki, K. Shimono, N. Kamo, M. Demura, and H. Kandori. 2005. Hydrogen-bonding alterations of the protonated Schiff base and water molecule in the chloride pump of Natronobacterium pharaonis. Biochemistry. 44:12279-12286.
29. Michel, H., and D. Oesterhelt. 1976. Light-induced changes of the pH gradient and themembrane potential in H. halobium. FEBS Lett. 65:175-178.
30. Nagel, G., B. Kelety, B. Mockel, G. Buldt, and E. Bamberg. 1998. Voltage dependence of proton pumping by bacteriorhodopsin is regulated by the voltage-sensitive ratio of M1 to M2. Biophys. J. 74:403-412.
31. Luecke, H., B. Schobert, H. T. Richter, J. P. Cartailler, and J. K. Lanyi. 1999. Structural changes in bacteriorhodopsin during ion transport at 2 Å resolution. Science. 286:255-261.
32. Lanyi, J. K., and H. Luecke. 2001. Bacteriorhodopsin. Curr. Opin. Struct. Biol. 11:415-419.
33. Robinson, R. A., and R. H. Stokes. 1959. Electrolyte Solution, 2nd ed. Butterworth, London.
34. Israelachvili, J. 1992. Intermolecular and Surface Forces. Academic Press, New York.