Enhanced differentiation of three-gene-reprogrammed induced pluripotent stem cells into adipocytes via adenoviral-mediated PGC-1α over expression

International Journal of Molecular Sciences

Volumn 12, Issue 11, 2011, Pages 7554-7568

  Huang, Pin I ^a,b   Chou, Yueh Ching ^a,b,c   Chang, Yuh Lih ^a,b   Chien, Yueh ^a,b   Chen, Kuan Hsuan ^a,b   Song, Wen Shin ^a,d   Peng, Chi Hsien ^a,e   Chang, Chin Hong ^f   Lee, Shin Da ^g   Lu, Kai Hsi ^a,d   Chen, Yi Jen ^a,b   Kuo, Chia Hua ^h   Hsu, Chuan Chih ^f   Lee, Hsin Chen ^a   Yung, Ming Chi ^a,i  

^a NATIONAL YANG MING UNIVERSITY   (Taiwan)

^b TAIPEI VETERANS GENERAL HOSPITAL   (Taiwan)

^c TAIPEI MEDICAL UNIVERSITY   (Taiwan)

^d CHENG HSIN GENERAL HOSPITAL   (Taiwan)

^e SHIN KONG WU HO SU MEMORIAL HOSPITAL   (Taiwan)

^f CHI MEI MEDICAL CENTER   (Taiwan)

^g CHINA MEDICAL UNIVERSITY   (Taiwan)

^h UNIVERSITY OF TAIPEI   (Taiwan)

ⁱ TAIWAN ADVENTIST HOSPITAL   (Taiwan)

Author keywords

Brown adipocyte; C MYC; Induced pluripotent stem cell; Peroxisome proliferator activated receptor gamma coactivator 1

Indexed keywords

ADENOVIRUS VECTOR; ALKALINE PHOSPHATASE; CYTOCHROME C; KRUPPEL LIKE FACTOR 4; NUCLEAR RESPIRATORY FACTOR 1; OCTAMER TRANSCRIPTION FACTOR 4; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA COACTIVATOR 1ALPHA; REACTIVE OXYGEN METABOLITE; TRANSCRIPTION FACTOR SOX2; UNCOUPLING PROTEIN 1; UNCOUPLING PROTEIN 2; ION CHANNEL; KRUPPEL LIKE FACTOR; MITOCHONDRIAL PROTEIN; MITOCHONDRIAL UNCOUPLING PROTEIN; MITOCHONDRIAL UNCOUPLING PROTEIN 2; NRF1 PROTEIN, MOUSE; POU5F1 PROTEIN, MOUSE; PPARGC1A PROTEIN, MOUSE; SOX2 PROTEIN, MOUSE; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR SOX;

ADIPOCYTE; ARTICLE; BROWN ADIPOSE TISSUE; CELL DIFFERENTIATION; CONTROLLED STUDY; EMBRYONIC STEM CELL; GENE; GENE EXPRESSION PROFILING; GENE OVEREXPRESSION; KLF4 GENE; MICROARRAY ANALYSIS; MITOCHONDRIAL MASS; MITOCHONDRION; OCT4 GENE; OSTEOCYTE; OXYGEN CONSUMPTION; PLURIPOTENT STEM CELL; PROTEIN EXPRESSION; SOX2 GENE; UPREGULATION; VIRAL GENE DELIVERY SYSTEM; WHITE ADIPOSE TISSUE; ADENOVIRIDAE; ANIMAL; BONE DEVELOPMENT; CYTOLOGY; GENETICS; METABOLISM; MOUSE;

ADENOVIRIDAE; ADIPOCYTES; ANIMALS; CELL DIFFERENTIATION; CYTOCHROMES C; INDUCED PLURIPOTENT STEM CELLS; ION CHANNELS; KRUPPEL-LIKE TRANSCRIPTION FACTORS; MICE; MITOCHONDRIA; MITOCHONDRIAL PROTEINS; NUCLEAR RESPIRATORY FACTOR 1; OCTAMER TRANSCRIPTION FACTOR-3; OSTEOGENESIS; REACTIVE OXYGEN SPECIES; SOXB1 TRANSCRIPTION FACTORS; TRANSCRIPTION FACTORS; UP-REGULATION;

EID: 82255181749     PISSN: None     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms12117554     Document Type: Article

References (29)

1. Chiou, S.H.; Kao, C.L.; Peng, C.H.; Chen, S.J.; Tarng, Y.W.; Ku, H.H.; Chen, Y.C.; Shyr, Y.M.; Liu, R.S.; Hsu, C.J., et al. A novel in vitro retinal differentiation model by co-culturing adult human bone marrow stem cells with retinal pigmented epithelium cells. Biochem. Biophys. Res. Commun. 2005, 326, 578-585.
2. Yang, D.C.; Tsay, H.J.; Lin, S.Y.; Chiou, S.H.; Li, M.J.; Chang, T.J.; Hung, S.C. CAMP/PKA regulates osteogenesis, adipogenesis and ratio of RANKL/OPG mRNA expression in mesenchymal stem cells by suppressing leptin. PLoS One 2008, 3, e1540.
3. Lin, H.T.; Kao, C.L.; Lee, K.H.; Chang, Y.L.; Chiou, S.H.; Tsai, F.T.; Tsai, T.H.; Sheu, D.C.; Ho, L.L.; Ku, H.H. Enhancement of insulin-producing cell differentiation from embryonic stem cells using pax4-nucleofection method. World J. Gastroenterol. 2007, 13, 1672-1679.
4. Okita, K.; Ichisaka, T.; Yamanaka, S. Generation of germline-competent induced pluripotent stem cells. Nature 2007, 448, 313-317.
5. Takahashi, K.; Yamanaka, S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 2006, 126, 663-676.
6. Li, H.Y.; Chien, Y.; Chen, Y.J.; Chen, S.F.; Chang, Y.L.; Chiang, C.H.; Jeng, S.Y.; Chang, C.M.; Wang, M.L.; Chen, L.K., et al. Reprogramming induced pluripotent stem cells in the absence of c-myc for differentiation into hepatocyte-like cells. Biomaterials 2011, 32, 5994-6005.
7. Puigserver, P.; Wu, Z.; Park, C.W.; Graves, R.; Wright, M.; Spiegelman, B.M. A cold-inducible coactivator of nuclear receptors linked to adaptive thermogenesis. Cell 1998, 92, 829-839.
8. Handschin, C.; Spiegelman, B.M. Peroxisome proliferator-activated receptor gamma coactivator 1 coactivators, energy homeostasis, and metabolism. Endocr. Rev. 2006, 27, 728-735.
9. Finck, B.N.; Kelly, D.P. PGC-1 coactivators: inducible regulators of energy metabolism in health and disease. J. Clin. Invest. 2006, 116, 615-622.
10. Wu, Z.; Puigserver, P.; Andersson, U.; Zhang, C.; Adelmant, G.; Mootha, V.; Troy, A.; Cinti, S.; Lowell, B.; Scarpulla, R.C., et al. Mechanisms controlling mitochondrial biogenesis and respiration through the thermogenic coactivator PGC-1. Cell 1999, 98, 115-124.
11. Lin, J.; Wu, P.H.; Tarr, P. T.; Lindenberg, K.S.; St-Pierre, J.; Zhang, C.Y.; Mootha, V.K.; Jager, S.; Vianna, C.R.; Reznick, R.M., et al. Defects in adaptive energy metabolism with CNS-linked hyperactivity in PGC-1alpha null mice. Cell 2004, 119, 121-135.
12. Leone, T.C.; Lehman, J.J.; Finck, B.N.; Schaeffer, P.J.; Wende, A.R.; Boudina, S.; Courtois, M.; Wozniak, D.F.; Sambandam, N.; Bernal-Mizrachi, C., et al. PGC-1alpha deficiency causes multisystem energy metabolic derangements: muscle dysfunction, abnormal weight control and hepatic steatosis. PLoS Biol. 2005, 3, e101.
13. Uldry, M.; Yang, W.; St-Pierre, J.; Lin, J.; Seale, P.; Spiegelman, B.M. Complementary action of the PGC-1 coactivators in mitochondrial biogenesis and brown fat differentiation. Cell Metab. 2006, 3, 333-341.
14. Chen, S.J.; Chang, C.M.; Tsai, S.K.; Chang, Y.L.; Chou, S.J.; Huang, S.S.; Tai, L.K.; Chen, Y.C.; Ku, H.H.; Li, H.Y., et al. Functional improvement of focal cerebral ischemia injury by subdural transplantation of induced pluripotent stem cells with fibrin glue. Stem Cells Dev. 2010, 19, 1757-1767.
15. Kao, C.L.; Tai, L.K.; Chiou, S.H.; Chen, Y.J.; Lee, K.H.; Chou, S.J.; Chang, Y.L.; Chang, C.M.; Chen, S.J.; Ku, H.H., et al. Resveratrol promotes osteogenic differentiation and protects against dexamethasone damage in murine induced pluripotent stem cells. Stem Cells Dev. 2010, 19, 247-258.
16. Chang, Y.L.; Chen, S.J.; Kao, C.L.; Hung, S.C.; Ding, D.C.; Yu, C.C.; Chen, Y.J.; Ku, H.H.; Lin, C.P.; Lee, K.H., et al. Docosahexaenoic acid promotes dopaminergic differentiation in induced pluripotent stem cells and inhibits teratoma formation in rats with parkinson-like pathology. Cell Transplant. 2011, In Press.
17. Pelengaris, S.; Khan, M.; Evan, G. C-MYC: more than just a matter of life and death. Nat. Rev. Cancer 2002, 2, 764-776.
18. Nakagawa, M.; Takizawa, N.; Narita, M.; Ichisaka, T.; Yamanaka, S. Promotion of direct reprogramming by transformation-deficient Myc. Proc. Natl. Acad. Sci. USA 2010, 107, 14152-14157.
19. Tsuji, O.; Miura, K.; Okada, Y.; Fujiyoshi, K.; Mukaino, M.; Nagoshi, N.; Kitamura, K.; Kumagai, G.; Nishino, M.; Tomisato, S., et al. Therapeutic potential of appropriately evaluated safe-induced pluripotent stem cells for spinal cord injury. Proc. Natl. Acad. Sci. USA 2010, 107, 12704-12709.
20. Bioconductor. Available online: http://www.bioconductor.org/ (accessed on 15 June 2011).
21. The R Project for Statistical Computing. Available online: http://www.r-project.org/ (accessed on 15 June 2011).
22. DChip Software: Analysis and visualization of gene expression and SNP microarrays. Available online: http://biosun1.harvard.edu/complab/dchip/ (accessed on 10 June 2011).
23. DAVID Bioinformatics Resources 6.7 interface. Available online: http://david.abcc.ncifcrf.gov/ (accessed on 30 June 2011).
24. Vankoningsloo, S.; Piens, M.; Lecocq, C.; Gilson, A.; de Pauw, A.; Renard, P.; Demazy, C.; Houbion, A.; Raes, M.; Arnould, T. Mitochondrial dysfunction induces triglyceride accumulation in 3T3-L1 cells: role of fatty acid beta-oxidation and glucose. J. Lipid Res. 2005, 46, 1133-1149.
25. Maassen, J.A. Mitochondrial dysfunction in adipocytes: the culprit in type 2 diabetes? Diabetologia 2006, 49, 619-620.
26. Park, I.H.; Zhao, R.; West, J.A.; Yabuuchi, A.; Huo, H.; Ince, T.A.; Lerou, P.H.; Lensch, M.W.; Daley, G.Q. Reprogramming of human somatic cells to pluripotency with defined factors. Nature 2008, 451, 141-146.
27. Yu, J.; Vodyanik, M.A.; Smuga-Otto, K.; Antosiewicz-Bourget, J.; Frane, J.L.; Tian, S.; Nie, J.; Jonsdottir, G.A.; Ruotti, V.; Stewart, R., et al. Induced pluripotent stem cell lines derived from human somatic cells. Science 2007, 318, 1917-1920.
28. Takahashi, K.; Tanabe, K.; Ohnuki, M.; Narita, M.; Ichisaka, T.; Tomoda, K.; Yamanaka, S. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 2007, 131, 861-872.
29. Tseng, Y.H.; Kokkotou, E.; Schulz, T.J.; Huang, T.L.; Winnay, J.N.; Taniguchi, C.M.; Tran, T.T.; Suzuki, R.; Espinoza, D.O.; Yamamoto, Y., et al. New role of bone morphogenetic protein 7 in brown adipogenesis and energy expenditure. Nature 2008, 454, 1000-1004.