Modelling the neurovascular unit and the blood-brain barrier with the unique function of pericytes

Current Neurovascular Research

Volumn 8, Issue 4, 2011, Pages 258-269

  Vandenhaute, Elodie ^a,b,c   Dehouck, Lucie ^a,b,c   Boucau, Marie Christine ^a,b,c   Sevin, Emmanuel ^a,b,c   Uzbekov, Rustem ^d   Tardivel, Meryem ^e   Gosselet, Fabien ^a,b,c   Fenart, Laurence ^a,b,c   Cecchelli, Roméo ^a,b,c   Dehouck, Marie Pierre ^a,b,c  

^a UNIV LILLE   (France)

^b UNIVERSITÉ D'ARTOIS   (France)

^c IMPRT IFR114   (France)

^d UNIVERSITÉ DE TOURS   (France)

^e UAR2014 Us 41 Plateformes Lilloises en Biol S BioImaging Ctr Lille   (France)

Author keywords

Blood brain barrier; Endothelial cells; In vitro models; Neurovascular unit; Pericytes; Three cell type model

Indexed keywords

GAMMA GLUTAMYLTRANSFERASE; GLYCOPROTEIN P;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BLOOD BRAIN BARRIER; BRAIN BLOOD VESSEL; CELL CULTURE; CELL MEMBRANE PERMEABILITY; CELL POPULATION; CONTROLLED STUDY; ENDOTHELIUM CELL; ENZYME ACTIVITY; GLIA CELL; IN VITRO STUDY; NONHUMAN; PERICYTE; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; SMOOTH MUSCLE FIBER;

EID: 82155191671     PISSN: 15672026     EISSN: 18755739     Source Type: Journal    
DOI: 10.2174/156720211798121016     Document Type: Article

References (36)

1. Cecchelli R, Berezowski V, Lundquist S, et al. Modelling of the blood-brain barrier in drug discovery and development. Nat Rev Drug Discov 2007;6(8):650-61. (Pubitemid 47202487)
2. Fisher M. Pericyte signaling in the neurovascular unit. Stroke 2009;40(3 Suppl):S13-5.
3. Deli MA, Abraham CS, Kataoka Y, Niwa M. Permeability studies on in vitro blood-brain barrier models: physiology, pathology, and pharmacology. Cell Mol Neurobiol 2005;25(1):59-127. (Pubitemid 40558585)
4. Prieto P, Blaauboer BJ, de Boer AG, et al. Blood-brain barrier in vitro models and their application in toxicology. The report and recommendations of ECVAM Workshop 49. Altern Lab Anim 2004;32(1):37-50. (Pubitemid 38524900)
5. Banumathi E, Haribalaganesh R, Babu SS, Kumar NS, Sangiliyandi G. High-yielding enzymatic method for isolation and culture of microvascular endothelial cells from bovine retinal blood vessels. Microvasc Res 2009;77(3):377-81.
6. Cecchelli R, Coisne C, Dehouck L, et al. In: Dermietzel R, Spray DC, Nedergaard M, Eds. Blood-Brain Barriers: From Ontogeny to Artificial Interfaces. Weinheim, Wiley-VCH. 2006: 337-55.
7. Lundquist S, Renftel M. The use of in vitro cell culture models for mechanistic studies and as permeability screens for the blood-brain barrier in the pharmaceutical industry-background and current status in the drug discovery process. Vascul Pharmacol 2002;38(6):355-64. (Pubitemid 36398257)
8. Lai CH, Kuo KH. The critical component to establish in vitro BBB model: Pericyte. Brain Res Brain Res Rev 2005;50(2):258-65.
9. Bonkowski D, Katyshev V, Balabanov RD, Borisov A, Dore-Duffy P. The CNS microvascular pericyte: pericyte-astrocyte crosstalk in the regulation of tissue survival. Fluids Barriers CNS 2011;8(1):8.
10. Bruinsma IB, Wilhelmus MM, Kox M, et al. Apolipoprotein E protects cultured pericytes and astrocytes from D-Abeta(1-40)-mediated cell death. Brain Res 2010;1315:169-80.
11. Bell RD, Winkler EA, Sagare AP, et al. Pericytes control key neurovascular functions and neuronal phenotype in the adult brain and during brain aging. Neuron 2010;68(3):409-27.
12. Yemisci M, Gursoy-Ozdemir Y, Vural A, et al. Pericyte contraction induced by oxidative-nitrative stress impairs capillary reflow despite successful opening of an occluded cerebral artery. Nat Med 2009;15(9):1031-7.
13. Dehouck MP, Meresse S, Delorme P, Fruchart JC, Cecchelli R. An easier, reproducible, and mass-production method to study the blood-brain barrier in vitro. J Neurochem 1990;54(5):1798-801.
14. Shine HD, Haber B. Immunocytochemical localization of gamma-glutamyl transpeptidase in the rat CNS. Brain Res 1981;217(2):339-49. (Pubitemid 11067803)
15. Berezowski V, Landry C, Dehouck MP, Cecchelli R, Fenart L. Contribution of glial cells and pericytes to the m RNA profiles of P-glycoprotein and multidrug resistance-associated proteins in an in vitro model of the blood-brain barrier. Brain Res 2004;1018(1):1-9. (Pubitemid 38942854)
16. Dehouck M-P, Vandenhaute E, Dehouck L, et al. In: Aschner M, Suñol C, Bal-Price A, Eds. Cell Culture Techniques. Breinigsville, PA, USA, Springer. 2011: 145-60.
17. Nehls V, Drenckhahn D. The versatility of microvascular pericytes: from mesenchyme to smooth muscle? Histochemistry 1993; 99(1): 1-12. (Pubitemid 23058525)
18. Risau W, Dingler A, Albrecht U, Dehouck MP, Cecchelli R. Blood-brain barrier pericytes are the main source of gamma- glutamyltranspeptidase activity in brain capillaries. J Neurochem 1992;58(2):667-72.
19. Shimizu F, Sano Y, Maeda T, et al. Peripheral nerve pericytes originating from the blood-nerve barrier expresses tight junctional molecules and transporters as barrier-forming cells. J Cell Physiol 2008;217(2):388-99.
20. Armulik A, Genove G, Mae M, et al. Pericytes regulate the blood- brain barrier. Nature 2010;468(7323):557-61.
21. Nakagawa S, Deli MA, Kawaguchi H, et al. A new blood-brain barrier model using primary rat brain endothelial cells, pericytes and astrocytes. Neurochem Int 2009;54(3-4):253-63.
22. Armulik A, Abramsson A, Betsholtz C. Endothelial/pericyte interactions. Circ Res 2005;97(6):512-23. (Pubitemid 41361741)
23. Collett GD, Canfield AE. Angiogenesis and pericytes in the initiation of ectopic calcification. Circ Res 2005;96(9):930-8. (Pubitemid 40712792)
24. Kirton JP, Wilkinson FL, Canfield AE, Alexander MY. Dexamethasone downregulates calcification-inhibitor molecules and accelerates osteogenic differentiation of vascular pericytes: implications for vascular calcification. Circ Res 2006;98(10):1264-72. (Pubitemid 43948081)
25. Dore-Duffy P. Pericytes: pluripotent cells of the blood brain barrier. Curr Pharm Des 2008;14(16):1581-93. (Pubitemid 352002985)
26. Frey A, Meckelein B, Weiler-Guttler H, et al. Pericytes of the brain microvasculature express gamma-glutamyl transpeptidase. Eur J Biochem 1991;202(2):421-9.
27. Bendayan R, Ronaldson PT, Gingras D, Bendayan M. In situ localization of P-glycoprotein (ABCB1) in human and rat brain. J Histochem Cytochem 2006;54(10):1159-67. (Pubitemid 44434719)
28. Hellstrom M, Gerhardt H, Kalen M, et al. Lack of pericytes leads to endothelial hyperplasia and abnormal vascular morphogenesis. J Cell Biol 2001;153(3):543-53. (Pubitemid 34280141)
29. Daneman R, Zhou L, Kebede AA, Barres BA. Pericytes are required for blood-brain barrier integrity during embryogenesis. Nature 2010;468:562-6
30. Allsopp G, Gamble HJ. An electron microscopic study of the pericytes of the developing capillaries in human fetal brain and muscle. J Anat 1979;128(Pt 1):155-68. (Pubitemid 9086887)
31. Caruso RA, Fedele F, Finocchiaro G, et al. Ultrastructural descriptions of pericyte/endothelium peg-socket interdigitations in the microvasculature of human gastric carcinomas. Anticancer Res 2009;29(1):449-53.
32. Orlidge A, D'Amore PA. Inhibition of capillary endothelial cell growth by pericytes and smooth muscle cells. J Cell Biol 1987;105(3):1455-62. (Pubitemid 18029870)
33. Wakui S, Yokoo K, Muto T, et al. Localization of Ang-1, -2, Tie-2, and VEGF expression at endothelial-pericyte interdigitation in rat angiogenesis. Lab Invest 2006;86(11):1172-84. (Pubitemid 44624153)
34. Fernandez-Klett F, Offenhauser N, Dirnagl U, Priller J, Lindauer U. Pericytes in capillaries are contractile in vivo, but arterioles mediate functional hyperemia in the mouse brain. Proc Natl Acad Sci U S A 2010;107:22290-5.
35. Lee S, Zeiger A, Maloney JM, et al. Pericyte actomyosin-mediated contraction at the cell-material interface can modulate the microvascular niche. J Phys Condens Matter 2010;22(19):194115.
36. Kamouchi M, Ago T, Kitazono T. Brain pericytes: emerging concepts and functional roles in brain homeostasis. Cell Mol Neurobiol 2011;31(2):175-93.