Large-scale preparation and characterization of non-pegylated and pegylated superactive ovine leptin antagonist

Protein Expression and Purification

Volumn 81, Issue 2, 2012, Pages 186-192

  Niv Spector, Leonora ^a   Shpilman, Michal ^a   Boisclair, Yves ^b   Gertler, Arieh ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^b Cornell University ^*  (United States)

Author keywords

Bioassay; Leptin antagonist; Ovine; Recombinant; Superactive

Indexed keywords

DRUG DERIVATIVE; LEPTIN; LEPTIN RECEPTOR; MACROGOL DERIVATIVE; PEGYLATED LEPTIN; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CELL INCLUSION; CELL LINE; CELL PROLIFERATION; DRUG ANTAGONISM; ESCHERICHIA COLI; GENE VECTOR; GENETICS; HALF LIFE TIME; HUMAN; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; MAMMAL; METABOLISM; MOLECULAR GENETICS; MOUSE; MUTATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN BINDING; PROTEIN FOLDING; PROTEIN SECONDARY STRUCTURE; REPORTER GENE; SEQUENCE ALIGNMENT; SHEEP; SOLUBILITY;

AMINO ACID SEQUENCE; ANIMALS; CELL LINE, TRANSFORMED; CELL PROLIFERATION; CHROMATOGRAPHY, ION EXCHANGE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENES, REPORTER; GENETIC VECTORS; HALF-LIFE; HUMANS; INCLUSION BODIES; LEPTIN; MAMMALS; MICE; MOLECULAR SEQUENCE DATA; MUTATION; POLYETHYLENE GLYCOLS; PROTEIN BINDING; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; RECEPTORS, LEPTIN; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SHEEP; SOLUBILITY;

BOVINAE; ESCHERICHIA COLI; OVIS; RATTUS; SUIDAE;

EID: 81155148574     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.10.003     Document Type: Article

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