Nanostructured materials for enzyme immobilization and biosensors

The New Frontiers of Organic and Composite Nanotechnology

Volumn , Issue , 2008, Pages 355-394

  Andreescu, Silvana ^a   Njagi, John ^a   Ispas, Cristina ^a  

^a CLARKSON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80755174598     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1016/B978-008045052-0.50009-9     Document Type: Chapter

References (138)

1. Moriarty P. Nanostructured materials. Rep. Prog. Phys. 2001, 64:297-381.
2. Kim J., Grate J.W., Wang P. Nanostructures for enzyme stabilization. Chem. Eng. Sci. 2006, 61:1017-1026.
3. Hartmann M. Ordered mesoporous materials for bioadsorption and biocatalysis. Chem. Mater. 2005, 17:4577-4593.
4. Tischer W., Kasche V. Immobilized enzymes: crystals or carriers? Trends Biotechnol. 1999, 17:326-335.
5. David A.E., Wang N.S., Yang V.C. Chemically surface modified gel (CSMG) an xcellent enzyme-immobilization matrix for industrial processes. J. Biotechnol. 2006, 125:395-407.
6. Krajewska B. Application of chitin- and chitosan- based materials for enzyme immobilizations: a review. Enzy. Microb. Technol. 2004, 35:126-139.
7. Böyükbayram A.E., Kiralp S., Toppare L. Preparation of biosensors by immobilization of polyphenol oxidase in conducting copolymers and their use in determination of phenolic compounds in red wine. Biolectrochemistry 2006, 69:164-171.
8. Chibata I. Immobilized Enzymes: Research and Development Halsted Press Book ISBN 0470265310 1978, John Wiley &Sons, New York.
9. Hudson S., Magner E., Cooney J. Methodology for the immobilization of enzyme onto mesoporous materials. J. Phys. Chem. B 2005, 109:19496-194506.
10. Sljukic B., Banks C.E., Salter C. Electrochemically polymerised composites of multi-walled carbon nanotubes and poly(vinylferrocene) and their use as modified electrodes: application to glucose sensing. Analyst 2006, 131:670-677.
11. Pan D., Chen J., Yao S. An amperometric glucose biosensor based on glucose oxidase immobilized in electropolymerized poly(oaminophenol) and carbon nanotubes composite film on gold electrode. Anal. Sci 2005, 21(4):367-671.
12. Wang J., Musameh M. Carbon-nanotubes doped polypyrrole glucose biosensor. Anal. Chim. Acta 2005, 539:209-213.
13. Luo X.-L., Xu J.-J., Wang J-L., et al. Electrochemically deposited nanocomposite of chitosan and carbon nanotubes for biosensor application. Chem., Comm. 2005, 16:2169-2171.
14. Jia H., Zhu G., Wang P. Catalytic behaviors associated with enzymes attached to nanoparticles: the effect of particle mobility. Biotechnol. Bioeng. 2003, 84:406-414.
15. Chen J.-P., Su D.-R. Latex particles with thermo-flocculation and magnetic properties for immobilization of α-chymotrypsin. Biotechnol. Prog. 2001, 17:369-375.
16. Mena M.L., Yanez-Sedeno P., Pingarron J.M. comparison of different strategies for the construction of amperometric biosensors using gold nanoparticles-modified electrodes. Anal. Biochem. 2005, 336:20-27.
17. 3 magnetic nanoparticles. J. Am. Chem. Soc. 2003, 125:1684-1685.
18. 2 matrix. Anal. Chem. Acta 2003, 478:59-66.
19. Heilmann A., Teuscher N., Kiesow A. Nanoporous aluminum oxide as novel support material for enzyme biosensors. J. Nanosci. Nanotechnol 2003, 3:375-379.
20. Jia H.F., Zhu G.Y., Vugrinovich B. Enzyme-carrying polymeric nanofibers prepared via electrospinning for use as unique biocatalysts. Biotechnol. Prog. 2002, 18:1027-1032.
21. Kim B.C., Nair S., Kim J. Preparation of biocatalytic nanofibers with high activity and stability via enzyme aggregate coating on polymer nanofibers. Nanotechnology 2005, 16:S382-S388.
22. Xiang Y., Hu Y., Liu F. Glucose biosensor based on Au nanoparticles - conductive polyaniline nanocomposites. Biosens. Bioelectron. 2006, 21:1996-2000.
23. Xu Q., Mao C., Liu N.-N., et al. Direct electrochemistry of horseradish peroxidase based on biocompatible carboxymethyl chitosan-gold nanoparticle nanocomposites. Biosens. Bioelectron. 2006, 22:768-773.
24. Yang M., Yang Y., Liu Y. Platinum nanoparticles-doped sol-gel/carbon nanotubes composite electrochemical sensors and biosensors. Biosens. Bioelectron. 2006, 21(7):1125-1131.
25. Yang M., Yang Y., Yang Y. Microbiosensor for acetylcholine and choline based on electropolymerization/sol - gel derived composite membrane. Anal. Chim. Acta. 2005, 530(2):205-211.
26. Tan X.-C., Tian Y.-X., Cai P.-X. Glucose biosensor based on glucose oxidase immobilized in sol - gel chitosan/silica hybrid composite film on Prussian blue modified glass carbon electrode. Anal. Bioanal. Chem. 2005, 381(2):500-507.
27. Kim J., Grate J.W. Single enzyme nanoparticles armored by nanometer nanometer-scale organic/inorganic network. Nano Lett. 2003, 3:1219-1222.
28. Gerard M., Chaubey A., Malhotra B.D. Application of conducting polymers to biosensors. Biosens. Bioelectron. 2002, 5:345.
29. Andreescu S., Marty J.-L. Twenty years research in cholinesterase biosensors: from basic research to practical applications. Biomol. Eng. 2006, 23:1-15.
30. Andreescu S., Sadik O.A. Trends and challenges in biochemical sensors for clinical and environmental monitoring. Pure Appl. Chem. 2004, 76(4):861.
31. Bonnet C., Andreescu S., Marty J.-L. Adsorption: an easy and efficient immobilisation of acetylcholinesterase on screen-printed electrodes. Anal. Chim. Acta. 2003, 481:209.
32. Collings A.F., Caruso F. Biosensors: recent advances. Rep. Prog. Phys. 1997, 60(11):1397.
33. Andreescu S., Barthelmebs L., Marty J.L. Immobilization of acetylcholinesterase on screen-printed electrodes: comparative study between three immobilization methods and applications to the detection of organophosphorus insecticides. Anal. Chim. Acta. 2002, 464(2):171.
34. Baker S.E., Tse K.Y., Hindin E. Covalent functionalization for biomolecular recognition on vertically aligned carbon nanofibers. Chem Mater. 2005, 17(20):4971.
35. Loscher F., Ruckstuhl T., Jaworek T. Immobilization of biomolecules on Langmuir-Blodgett films of regenerative cellulose derivatives. Langmuir 1998, 14(10):2786.
36. Senaratne W., Andruzzi L., Ober C.K. Self-assembled monolayers and polymer brushes in biotechnology: current applications and future perspectives. Biomacromolecules. 2005, 6(5):2427.
37. Sigal G.B., Bamdad C., Barberis A. self-assembled monolayer for the binding and study of histidine tagged proteins by surface plasmon resonance. Anal. Chem. 1996, 68(3):490.
38. Scouten W.H., Luong J.H.T., Brown R.S. Enzyme or protein immobilization techniques for applications in biosensors design. Tibtech. 1995, 13:178-185.
39. Thévenot D.R., Toth K., Durst R.A. Electrochemical biosensors: recommended definitions and classification. Biosens. Bioelectron. 2001, 16:121-131.
40. Vamvakaki V., Tsagaraki K., Chaniotakis N. Carbon nanofiberbased glucose biosensor. Anal. Chem. 2006, 78:5538-5542.
41. Phadtare S., Vinod V.P., Wadgaonkar P.P. Free-standing nanogold membranes as scaffolds for enzyme immobilization. Langmuir 2004, 20:3717-3723.
42. Andreescu S., Marty J.L. Twenty years research in cholinesterase biosensors: from basic research to practical applications. Biomol. Eng. 2006, 23:1-15.
43. Gooding J.J., Hibbert D.B. The application of alkanethiol self-assembled monolayers to enzyme electrodes. Trends Anal. Chem. 1999, 18(8):525.
44. Chaki N.K., Vijayamohanan K. Self-assembled monolayers as tunable platform for biosensor applications. Biosens. Bioelectron. 2002, 17:1-12.
45. Wink Th., van Zuilen S.J., Bult A., et al. Self-assembled monolayers for biosensors. Analyst 1997, 122:43R-50R.
46. Fink S., van Veggel F.C.J.M., Reinhoudt D.N. Sensor functionalities in self-assembled monolayers. Adv. Mater. 2000, 12:1315-1328.
47. Saleemuddin M. Bioaffinity based immobilization of enzymes. Adv. Biochem. Eng. Biotechnol. 1999, 64:203-226.
48. Kumada Y., Tokunaga Y., Imanaka H. Screening and characterization of affinity peptide tags specific to polystyrene supports for the orientated immobilization of proteins. Biotechnol. Prog. 2006, 22:401-405.
49. Andreescu S., Bucur B., Marty J.-L. Affinity immobilization of tagged enzymes. Immobilization of Enzymes and Cells 2006, 97-106. (ISBN 1-58829-290-8), Humana Press, Series: Methods in Biotechnology. Second. J.M. Guisan (Ed.).
50. Andreescu S., Magearu V., Lougarre A. Immobilization of enzymes on screen-printed sensors via an histidine tail. Application to the detection of pesticides using modified cholinesterase. Anal. Lett. 2001, 34(4):429.
51. Hsiao H., Royer G.P. Immobilization of glycoenzymes through carbohydrate side chain. Arch. Biochem. Biophys. 1979, 198:379-385.
52. Woodward J., Wiseman A. The involvement of salt linkages in the stabilization of bakers yeast invertase. Biochim. Biophys. Acta. 1978, 527:8-16.
53. Saleemuddin M., Husain Q. Concanavalin A: useful ligand for glycoenzyme immobilization. Enzyme Microbiol. Technol. 1991, 13:290-299.
54. Song J.M., Vo-Dinh T. Miniature biochip system for detection of Escherichia coli O157:H7 based on antibody-immobilized capillary reactors and enzyme-linked immunosorbent assay. Anal. Chim. Acta 2004, 507:115-121.
55. Oh B.K., Chun B.S., Park K.W. Fabrication of protein G LB film for immunoglobulin G immobilization. Mater. Sci. Eng. C 2004, 24:65-69.
56. Wang A.A., Lee J., Jenikova G. Controlled assembly of multi-segment nanowires by histidine-tagged peptides. Nanotechnology 2006, 17:3375-3379.
57. Cosnier S., Mousty C., Gondran C. Entrapment of enzyme within organic and inorganic materials for biosensor applications: Compartive study. Mater. Sci. Eng. C 2006, 26:442-447.
58. Cosnier S. Affinity biosensors based on electroplymerized films. Electroanal. 2005, 17(9):1701-1705.
59. Brahim S., Narnesigh D., Guiseppi-Elie A. Polypyrrole-hydrogel composites for the construction of clinically important biosensors. Biosens. Bioelectron. 2002, 17:53-59.
60. Njagi J., Andreescu D., Andreescu S. Nanostructures for enzyme immobilization and biosensors 2006.
61. Wang J. Nanomaterial-based electrochemical biosensors. Analyst. 2005, 130:421-426.
62. Rao C.N.R., Satishkumar B.C., Govindarai A. Nanotubes. Chemphyschem 2001, 2(2):78-105.
63. Baughmman R.H., Zaknidov A.A., Heer W.A. Carbon nanotubes-the route toward applications. Science 2002, 297:787-792.
64. Wang J. Carbon-nanotube based electrochemical biosensors: a review. Electroanalysis 2005, 17(1):7.
65. Davis J.J., Coleman K.S., Azamian B.R. Chemical and biochemical sensing with modified single walled carbon nanotubes. Chem. Eur. J. 2003, 9:3732.
66. Jianrong C., Yuqing M., Nongyue H. Nanotechnology and biosensors. Biotechnol. Advances. 2004, 22:505-518.
67. Barone P.W., Baik S., Heller D.A. Near-infrared optical sensors based on single-walled carbon nanotubes. Nat. Mater. 2005, 4(1):86-92.
68. Ziegler K.J. Developing implantable optical biosensors. Trends in Biotechnol. 2005, 23(9):440-444.
69. Sotiropoulou S., Gavalas V., Vamvakaki V. Novel carbon materials in biosensors systems. Biosens. Bioelectron. 2003, 18:211-215.
70. Guiseppi-Elie A., Lei C., Baughman R.H. Direct electron transfer of glucose oxidase on carbon nanotubes. Nanotechnology 2002, 13:559-564.
71. Wang J., Musameh M. Enzyme dispersed carbon-nanotube electrodes: a needle microsensor for monitoring glucose. Analyst. 2003, 128:1382.
72. Rubianes M.D., Rivas G.A. Carbon nanotubes paste electrodes. Electrochem. Comm. 2003, 5:689-694.
73. Perez B., Pumera M., del Valle M., et al. Glucose biosensor based on carbon nanotube epoxy composites. J. Nanosci. Nanotechnol. 2005, 5(10):1694-1698.
74. Liu Y., Wang M., Zhao F. The direct electron transfer of glucose oxidase and glucose biosensor based on carbon nanotubes/chitosan matrix. Biosens. Bioelectron. 2005, 21:984-988.
75. Wang J., Musameh M. Carbon nanotube/Teflon composite electrochemical sensors and biosensors. Anal. Chem. 2003, 75:2075.
76. Zhang M., Gorski W. Electrochemical sensing based on redox mediation at carbon nanotubes. Anal. Chem. 2005, 77:3960-3965.
77. Musameh M., Wang J., Merkoci A. Low-potential stable NADH detection at carbon-nanotube-modified glassy carbon electrodes. Electrochem. Comm. 2002, 4:743-746.
78. Joshi K.A., Tang J., Haddon R. A disposable biosensors for organophosphorus nerve agents based on carbon nanotubes modified thick film strip electrodes. Electroanalysis 2005, 17:54-58.
79. Lin Y.H., Lu F., Wang J. Disposable carbon nanotube modified screen-printed biosensor for amperometric detection of organophosphorus pesticides and nerve agents. Electroanalysis 2004, 16:145-149.
80. Wang S.G., Zhang Q., Wang R. A novel multi-walled carbon nanotube-based biosensor for glucose detection. Biochem. Biophys. Res. Comm. 2003, 311:572-576.
81. Lei C., Shin Y., Liu J. Entrapping enzyme in functionalized nanoporous support. J. Am. Chem. Soc. 2002, 124:11242-11243.
82. Bahr J.L., Tour J.M. Covalent chemistry of single-wall carbon nanotubes. J. Mater. Chem. 2002, 12:1952.
83. Lin Y., Lu F., Tu F. Glucose biosensors based on carbon nanotubes nanoelectrode ensemblies. Nano Lett. 2004, 4(2):191-195.
84. Ravidran S., Chaudhary S., Colburn B. Covalent coupling of quantum dots to multiwalled carbon nanotubes for electronic device applications. Nano Lett. 2003, 2(4):447-453.
85. Carrillo A., Swartz J.A., Gamba J.M. Non-covalent functionalization of graphite and carbon nanotubes with polymer multilayers and gold nanoparticles. Nano Lett. 2003, 3(10):1437-1440.
86. Musameh M., Wang J. Magnetically induced carbon nanotubemediated control of electrochemical reactivity. Langmuir 2005, 21:8565-8568.
87. Kim J., Jia H., Wang P. Challenges in biocatalysis for enzymebased biofuel cells. Biotechnol. Advances 2006, 24:296-308.
88. Baker S.E., Colavita P.E., Tse K-Y., et al. Functionalized vertically aligned carbon nanofibers as scaffolds for immobilization and electrochemical detection of redox-active proteins. Chem. Mater. 2006, 18:4415-4422.
89. Ye P., Xu Z.K., Wu J. Nanofibrous membranes containing reactive groups: electrospinning from poly(acrylonitrile-co-maleic acid) for lipase immobilization. Macromolecules 2006, 39(3):1041-1045.
90. Sawicka K., Gouma P., Simon S. Electrospun biocomposite nanofibers for urea biosensing. Sens. Actuators B. 2005, 108:585-588.
91. Patel A.C., Li S.X., Yuan J.M. In situ encapsulation of horseradish peroxidase in electrospun porous silica fibers for potential biosensor applications. Nano Lett. 2006, 6(5):1042-1046.
92. Chen W.W., Yao H., Tzang C.H. Silicon nanowires for high-sensitivity glucose detection. Appl. Phys. Lett. 2006, 88:21.
93. Mukhopadhyay K., Padtare S., Vinod V.P. Gold nanoparticles assembled on amine-functionalized Na-Y zeolite: a biocompatible surface for enzyme immobilization. Langmuir 2003, 19:3858-3863.
94. Lei C.X., Hu S.Q., Shen G.L. Immobilization of horseradish peroxidase to nano-Au monolayer modified chitosan-entrapped carbon paste electrode for the detection of hydrogen peroxide. Talanta 2003, 59(5):981-988.
95. Wu Z.S., Zhou G.Z., Jiang J.H. Gold colloid-bienzyme conjugates for glucose detecting surface-enhanced raman Scatterring. Talanta 2006, 70(3):533-539.
96. Katz E., Willner I., Wang J. Electroanalytical and Bioelectroanalytical systems based on metal and semiconductor nanoparticles. Electroanalysis 2004, 16(1-2):19.
97. Rossi L.M., Quach A.D., Rosenzweig Z. Glucose oxidasemagnetite nanoparticle bioconjugate for glucose sensing. Anal. Bioanal. Chem. 2004, 380(4):606.
98. Pardo-Yissar V., Katz E., Wasserman J. Acetylcholine esterase-labeled CdS nanoparticles on electrodes: photoelectrochemical sensing of the enzyme inhibitors. J. Am. Chem Soc. 2003, 125(3):622-623.
99. Katz E., Zayats M., Willner I. Controlling the direction of photocurrents by means of CdS nanoparticles and cytochrome c-mediated biocatalytic cascades. Chem. Comm. 2006, 13:1396-1397.
100. Ji X.S., Zheng J.Y., Xu J.M. (CdSe)ZnS quantum dots and organophosphorus hydrolase bioconjugate as biosensors for detection of paraoxon. J. PhysChem. B 2005, 109(9):3793-3799.
101. Huang Y.X., Zhang W.J., Xiao H. An electrochemical investigation of glucose oxidase at CdS nanoparticles modified electrode. Biosens. Bioelectron. 2005, 21(5):817-821.
102. Liu Z., Liu Y., Yang H. A phenol biosensor based on immobilizing tyrosinase to modified core-shell magnetic nanoparticles supported at carbon paste electrode. Anal. Chim. Acta 2005, 533:3-9.
103. Hong J., Gong P.-J., Yu J.H. Conjugation of alfa-chymotrypsin on polymeric hydrophobic nanolayer covering magnetic nanoparticles. Mol. Catal. B-Enzym 2006, 42(3-4):99-105.
104. Albareda-Sirvent M., Merkoci A., Alegret S. Configurations used in the design of screen-printed enzymatic biosensors. a review. Sens. Actuators. B 2000, 69(1-2):153-163.
105. Cespedes F., Alegret S. New materials for electrochemical sensing II. Rigid carbon-polymer biocomposites. Trends in Anal. Chem. 2000, 19(4):276-284.
106. Cosnier S. Biomolecule immobilization on electrode surfaces by entrapment or attachment to electrochemically polymerized film. A review. Biosens. Bioelectron. 1999, 14:443-456.
107. Yuqing M., Jianrong C., Xiaohua W. Using electropolymerized non-conducting polymers to develop enzyme amperometric biosensors. Trends in Biotechnol. 2004, 22(5):227-231.
108. Fan Q., Shan D., Xue H. Amperometric phenol biosensor based on laponite clay-Chitosan nanocomposite matrix. Biosens. Bioelectron 2007, 22:816-821.
109. 2 nanostructured films. Mater. Sci. Eng. C 2006, 26(5-7):947-951.
110. 2 nanostructured films to modify electrodes for assembling of new electrochemical probes. Sens. Actuators. B. 2005, 111-112:441-449.
111. Carrara S., Bavastrello V., Ricci D. Improved nanocompotite materials foir biosensor applications investigated by electrochemical impedance spectroscopy. Sens. Actuators, B. 2005, 109:221-226.
112. Butterfield D.A., Bhattacharyya D., Daunert S. Catalytic biofunctional membranes containing site-specifically immobilized enzyme arrays: a review. J. membrane science. 2001, 181(1):28-37.
113. Delouise L.A., Miller B.L. Enzyme immobilization in porous silicon: Quantitative analysis of the kinetic parameters for glutathione-S-transferases. Anal. Chem. 2005, 77(7):1950-1956.
114. Myler S., Collyer S.D., Bridge K.A. Ultra-thin-polysiloxanefilm-composite membranes for the optimisation of amperometric oxidase enzyme electrodes. Biosens. Bioelectron. 2002, 17:35-43.
115. Hernandez-Velez M. Nanowires and 1D arrays fabrication: an overview. Thin Solid Films. 2006, 495:51-63.
116. Schöning M.J., Kurowski A., Thust M. Capacitive microsensors for biochemical sensing based on porous silicon technology. Sens Actuators 2000, 64:59-64.
117. Darder M., Aranda P., Hernández-Vález M. Encapsulation of enzymes in alumina membranes of controlled pore size. Thin Solid Films. 2006, 495:321-326.
118. Crespilho F.N., Ghica M.E., Florescu M. A strategy for enzyme immobilization on layer-by-layer dendrimer-gold nanoparticle electrocatalytic membrane incorporating redox mediator. Electrochem. Commun. 2006, 8:1665-1670.
119. Piletsky S., Piletska E., Bossi A. Surface functionalization of porous polypropylene membranes with polyaniline for protein immobilization. Biotectnol. Bioeng. 2003, 82(1):86-92.
120. Wang Y., Caruso F. Enzyme encapsulation in nanoporous silica spheres. Chem. Comm. 2004, 13:1528-1529. issue.
121. Wang Y., Caruso F. Mesoporous silica spheres for enzyme immobilization and encapsulation. Chem. Mater. 2005, 17:953-961.
122. Sotiropoulou S., Vamvakaki V., Chaniotakis N.A. Stabilization of enzymes in nanoporous materials for biosensor applications. Biosens. Bioelectron. 2005, 20:1674-1679.
123. Ma H., He J., Evans D.G. Immobilization of lipase in mesoporous reactor based on MCM-41. J. Mol. Catal. Enzym. 2004, 30:209-217.
124. Blanco R.M., Terreros P., Fernandez-Perez M. Functionalization of mesoporous silica for lipase immobilization characterization of the support and the catalysts. J. Mol. Catal. B-Enzym 2004, 30:83-93.
125. Avnir D., Coradin T., Lev O. Recent bio-applications of sol-gel materials. J. Mater. Chem. 2005, 16:1013-1030.
126. Vinu A., Murugesan V., Tangermann O. Adsorption of cytochrome c on mesoporous molecular sieves: influence of pH, pore diameter, and aluminum incorporation. Chem. Mater. 2004, 16:3056-3065.
127. Diaz J.F., Balkus K.J. Enzyme immobilization in MCM-41 molecular sieve. J. Molec. Catal. B. Enzymatic 1996, 2:115-126.
128. Washmon-Kriel L., Jimerez V.L., Balkus K.J. Cytochrome c immobilization into mesoporous molecular sieves. J. Molec. Catal. B. Enzym. 2000, 10:453-469.
129. Takahashi H., Li B., Sasaki T., et al. Immobilized enzymes in ordered mesoporous silica materials and improvement of their stability and catalytic activity in an organic solvent. Micropor. Mesopor. Mater 2001, 44-45:755-762.
130. Sun J., Zhang H., Tian R., et al. Ultrafast enzyme immobilization over large-pore nanoscale mesoporous silica particles. Chem. Comm. 2006, 12:1322-1324. issue.
131. Yang X.-Y., Li Z.-Q., Liu B. "Fish in net" encapsulation of enzymes in macroporous cages as stable, reusable and active heterogeneous biocatalysts. Adv. Mater. 2006, 18:410-414.
132. Yang M., Yang Y., Qu F. Attachment of nickel hexacyanoferrates nanoparticles on carbon nanotubes: preparation, characterization and bioapplication. Anal. Chim. Acta. 2006, 571(2):211-217.
133. Wang S.G., Zhang Q., Wang R. A novel multi-walled carbon nanotube-based biosensor for glucose detection. Biochem. Biophys. Res. Commun. 2003, 311:572-576.
134. Agui L., Manso J., Yáñez-Sedeño P. Amperometric biosensor for hypoxanthine based on immobilized xanthine oxidase on nanocrystal gold-carbon paste electrodes. Sens. Actuators B. 2006, 113:272-280.
135. Shchipunov Y.A., Burtseva Y.V., Karpenko T.Y. Highly efficient immobilization of endo-1, 3β-d-glucanases (laminarinases) from marine mollusks in novel hybrid polysaccharidesilica nanocomposites with regulated composition. J. Mol. Catal. B: Enzym. 2006, 40:16-23.
136. Kim J.-K., Park J.-K., Kim H.-K. Synthesis and characterization of nanoporous silica support for enzyme immobilization. Colloids Surf. A Physicochem. Eng. Aspects. 2004, 241:113-117.
137. Xua J.-Z., Zhangb Y., Lia G.-X. An electrochemical biosensor constructed by nanosized silver particles doped sol-gel film. Mater. Sci. Eng. C. 2004, 24:833-836.
138. Chen J.-P., Lin W.-S. Sol-gel powders and supported sol-gel polymers for immobilization of lipase in ester synthesis. Enzyme Microb. Technol. 2003, 32:801-811.