Analytical aspects in doping control: Challenges and perspectives

Forensic Science International

Volumn 213, Issue 1-3, 2011, Pages 49-61

  Badoud, Flavia ^a,b   Guillarme, Davy ^a   Boccard, Julien ^a   Grata, Elia ^a,b   Saugy, Martial ^a,b   Rudaz, Serge ^a   Veuthey, Jean Luc ^a  

^a UNIVERSITY OF GENEVA   (Switzerland)

^b UNIVERSITY CENTER OF LEGAL MEDICINE   (Switzerland)

Author keywords

Doping control; Gas chromatography; Mass spectrometry; Proteins and peptides; Sample preparation; Ultra high pressure liquid chromatography

Indexed keywords

ACCURACY; ANALYTIC METHOD; ARTICLE; ATHLETE; CAPILLARY ZONE ELECTROPHORESIS; CHEMICAL COMPOSITION; CHEMICAL STRUCTURE; DOPING; DRUG CONTROL; FALSE NEGATIVE RESULT; FALSE POSITIVE RESULT; GAS CHROMATOGRAPHY; HEALTH CARE ORGANIZATION; HUMAN; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METABOLITE; PRIORITY JOURNAL; PROTEIN ANALYSIS; REACTION TIME; SENSITIVITY AND SPECIFICITY; SEPARATION TECHNIQUE; SPORTING EVENT; STANDARDIZATION; TANDEM MASS SPECTROMETRY; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY; URINALYSIS;

EID: 80655124636     PISSN: 03790738     EISSN: 18726283     Source Type: Journal    
DOI: 10.1016/j.forsciint.2011.07.024     Document Type: Article

References (119)

1. Hatton C.K. Beyond sports-doping headlines: the science of laboratory tests for performance-enhancing dugs. Pediatr. Clin. North Am. 2007, 54:713-733.
2. Mueller K.R. History of doping and doping control. Doping in Sports: Biochemical Principles, Effects and Analysis 2010, 1-23. Springer-Verlag, Heidelberg. D. Thieme, P. Hemmersbach (Eds.).
3. Bowers L.D. Analytical advances in detection of performance-enhancing compounds. Clin. Chem. 1997, 43:1299-1304.
4. Deutsch D.G. Gas chromatography mass spectrometry using tabletop instruments. Analytical Aspects of Drug Testing 1989, 87-128. John Wiley & Sons, New York. J.D. Winefordner (Ed.).
5. World Anti-Doping Agency (WADA), The World Anti-Doping Code. The 2011 Prohibited List, Montreal, 2011, (accessed June 2011). http://www.wada-ama.org/.
6. Kuuranne T. Phase-II metabolism of androgens and its relevance for doping control analysis. Doping in Sports: Biochemical Principles, Effects and Analysis 2010, 65-76. Springer-Verlag, Heidelberg. D. Thieme, P. Hemmersbach (Eds.).
7. Mueller K.R., Grosse J., Lang R., Thieme D. Chromatographic techniques: the basis of doping control. J. Chromatogr. B: Biomed. Sci. Appl. 1995, 674:1-11.
8. Hemmersbach P., Jetne A.H.H., Lund H.S. Determination of urinary norandrosterone excretion in females during one menstrual cycle by gas chromatography/mass spectrometry. Biomed. Chromatogr. 2006, 20:710-717.
9. Sottas P.E., Robinson N., Saugy M. The athlete's biological passport and indirect markers of blood doping. Doping in Sports: Biochemical Principles, Effects and Analysis 2010, 305-326. Springer-Verlag, Heidelberg. D. Thieme, P. Hemmersbach (Eds.).
10. World Anti-doping Agency (WADA), The World Anti-Doping Code. Minimal Required Performance Limits, Technical Document TD2010MRPL, Montreal, 2010, (accessed June 2011). http://www.wada-ama.org/.
11. World Anti-doping Agency (WADA), The World Anti-Doping Code. Identification Criteria for Qualitative Assays, Technical Document TD2010IDCR, Montreal, 2010, (accessed June 2011). http://www.wada-ama.org/.
12. Rivier L. Criteria for the identification of compounds by liquid chromatography-mass spectrometry and liquid chromatography-multiple mass spectrometry in forensic toxicology and doping analysis. Anal. Chim. Acta 2003, 492:69-82.
13. Couchman L., Morgan P.E. LC-MS in analytical toxicology: some practical considerations. Biomed. Chromatogr. 2011, 25:100-123.
14. Veuthey J.-L., Guillarme D., Rudaz S., Marchi I., Nguyen D., Saugy M., Mateus-Avois L. Fast analysis of doping agents by ultra performance liquid chromatography. GIT Lab. J. 2007, 5-6:14-15.
15. Thörngren J.-O., östervall F., Garle M. A high-throughput multicomponent screening method for diuretics, masking agents, central nervous system (CNS) stimulants and opiates in human urine by UPLC-MS/MS. J. Mass Spectrom. 2008, 43:980-992.
16. Badoud F., Grata E., Perrenoud L., Avois L., Saugy M., Rudaz S., Veuthey J.L. Fast analysis of doping agents in urine by ultra-high pressure liquid chromatography-quadrupole time-of-flight mass spectrometry: I. Screening analysis. J. Chromatogr. A 2009, 1216:4423-4433.
17. Guddat S., Thevis M., Thomas A., Schänzer W. Rapid screening of polysaccharide-based plasma volume expanders dextran and hydroxyethyl starch in human urine by liquid chromatography-tandem mass spectrometry. Biomed. Chromatogr. 2008, 22:695-701.
18. Thevis M., Sigmund G., Koch A., Guddat S., Maurer H.H., Schaenzer W. Doping control analysis of methoxyphenamine using liquid chromatography-tandem mass spectrometry. Eur. J. Mass Spectrom. 2008, 14:145-152.
19. Deventer K., Pozo O.J., Van Eenoo P., Delbeke F.T. Qualitative detection of diuretics and acidic metabolites of other doping agents in human urine by high-performance liquid chromatography-tandem mass spectrometry: comparison between liquid-liquid extraction and direct injection. J. Chromatogr. A 2009, 1216:5819-5827.
20. Polson C., Sarkar P., Incledon B., Raguvaran V., Grant R. Optimization of protein precipitation based upon effectiveness of protein removal and ionization effect in liquid chromatography-tandem mass spectrometry. J. Chromatogr. B 2003, 785:263-275.
21. Kole P.L., Venkatesh G., Kotecha J., Sheshala R. Recent advances in sample preparation techniques for effective bioanalytical methods. Biomed. Chromatogr. 2011, 25:199-217.
22. Kataoka H. Recent developments and applications of microextraction techniques in drug analysis. Anal. Bioanal. Chem. 2010, 396:339-364.
23. Bagheri H., Khalilian F., Ahangar L.E. Liquid-liquid-liquid microextraction followed by HPLC with UV detection for quantitation of ephedrine in urine. J. Sep. Sci. 2008, 31:3212-3217.
24. Zhang Z., Wang D., Zhang L., Du M., Chen G. Determination of diuretics in human urine by hollow fiber-based liquid-liquid-liquid microextraction coupled to high performance liquid chromatography. Analyst 2008, 133:1187-1194.
25. Marchi I., Rudaz S., Veuthey J.-L. Sample preparation development and matrix effects evaluation for multianalyte determination in urine. J. Pharm. Biomed. Anal. 2009, 49:459-467.
26. Pucci V., Di Palma S., Alfieri A., Bonelli F., Monteagudo E. A novel strategy for reducing phospholipids-based matrix effect in LC-ESI-MS bioanalysis by means of HybridSPE. J. Pharm. Biomed. Anal. 2009, 50:867-871.
27. Abdel-Rehim M. Recent advances in microextraction by packed sorbent for bioanalysis. J. Chromatogr. A 2010, 1217:2569-2580.
28. Claude B., Morin P., Bayoudh S., de Ceaurriz J. Interest of molecularly imprinted polymers in the fight against doping: extraction of tamoxifen and its main metabolite from urine followed by high-performance liquid chromatography with UV detection. J. Chromatogr. A 2008, 1196-1197:81-88.
29. Saito K., Yagi K., Ishizaki A., Kataoka H. Determination of anabolic steroids in human urine by automated in-tube solid-phase microextraction coupled with liquid chromatography-mass spectrometry. J. Pharm. Biomed. Anal. 2010, 52:727-733.
30. Kim S., Park J.-H., Myung S.-W., Lho D.-S. Determination of carphedon in human urine by solid-phase microextraction using capillary gas chromatography with nitrogen-phosphorus detection. Analyst 1999, 124:1559-1562.
31. Nageswara Rao R., Vali R.M., Ramachandra B., Maurya P.K. Rapid determination of rifaximin on dried blood spots by LC-ESI-MS. Biomed. Chromatogr. 2011, 25. 10.1002/bmc.1591.
32. Li W., Tse F.L.S. Dried blood spot sampling in combination with LC-MS/MS for quantitative analysis of small molecules. Biomed. Chromatogr. 2009, 24:49-65.
33. Majors R.E. New directions in whole blood analysis: dried blood spot analysis and beyond. LC-GC North Am. 2011, 29:14-27.
34. Peng S.-H., Segura J., Farre M., de la Torre X. Oral testosterone administration detected by testosterone glucuronidation measured in blood spots dried on filter paper. Clin. Chem. 2000, 46:515-522.
35. Mather J., Rainville P.D., Spooner N., Evans C.A., Smith N.W., Plumb R.S. Rapid analysis of dried blood spot samples with sub-2-μm LC-MS/MS. Bioanalysis 2011, 3:411-420.
36. Thomas A., Déglon J., Steimer T., Mangin P., Daali Y., Staub C. On-line desorption of dried blood spots coupled to hydrophilic interaction/reversed-phase LC/MS/MS system for the simultaneous analysis of drugs and their polar metabolites. J. Sep. Sci. 2010, 33:873-879.
37. Maurer H.H. Role of gas chromatography mass spectrometry with negative ion chemical ionization in clinical and forensic toxicology, doping control, and biomonitoring. Ther. Drug Monit. 2002, 24:247-254.
38. Leis H.J., Fauler G., Raspotnig G., Windischhofer W. Negative ion chemical ionization for the determination of methylphenidate in human plasma by stable isotope dilution gas chromatography/mass spectrometry. J. Mass Spectrom. 2000, 35:1100-1104.
39. Aebi B., Bernhard W. Advances in the use of mass spectral libraries for forensic toxicology. J. Anal. Toxicol. 2002, 26:149-156.
40. Krone N., Hughes B.A., Lavery G.G., Stewart P.M., Arlt W., Shackleton C.H.L. Gas chromatography/mass spectrometry (GC/MS) remains a pre-eminent discovery tool in clinical steroid investigations even in the era of fast liquid chromatography tandem mass spectrometry (LC/MS/MS). J. Ster. Biochem. Mol. Biol. 2010, 121:496-504.
41. Dallüge J., Beens J., Brinkman U.A.T. Comprehensive two-dimensional gas chromatography: a powerful and versatile analytical tool. J. Chromatogr. A 2003, 1000:69-108.
42. Silva A.I., Pereira H.M.G., Casilli A., Conceição F.C., Aquino Neto F.R. Analytical challenges in doping control: comprehensive two-dimensional gas chromatography with time of flight mass spectrometry, a promising option. J. Chromatogr. A 2009, 1216:2913-2922.
43. Mydlová-Memersheimerová J., Tienpont B., David F., Krupcík J., Sandra P. Gas chromatography of 209 polychlorinated biphenyl congeners on an extremely efficient nonselective capillary column. J. Chromatogr. A 2009, 1216:6043-6062.
44. Van Eenoo P., Van Gansbeke W., De Brabanter N., Deventer K., Delbeke F.T. A fast, comprehensive screening method for doping agents in urine by gas chromatography-triple quadrupole mass spectrometry. J. Chromatogr. A 2011, 1218:3306-3316.
45. Catlin D.H., Sekera M.H., Ahrens B.D., Starcevic B., Chang Y.-C., Hatton C.K. Tetrahydrogestrinone: discovery, synthesis, and detection in urine. Rapid Commun. Mass Spectrom. 2004, 18:1045-1049.
46. Parr M.K., Fußhöller G., Schlörer N., Opfermann G., Geyer H., Rodchenkov G., Schänzer W. Detection of [Delta]6-methyltestosterone in a " dietary supplement" and GC-MS/MS investigations on its urinary metabolism. Toxicol. Lett. 2011, 201:101-104.
47. de Boer D., Bernal M.E.G., van Ooyen R.D., Maes R.A.A. The analysis of trenbolone and the human urinary metabolites of trenbolone acetate by gas chromatography/mass spectrometry and gas chromatography/tandem mass spectrometry. Biol. Mass Spectrom. 1991, 20:459-466.
48. Saudan C., Saugy M. IRMS to detect doping in sports. The Encyclopedia of Mass Spectrometry 2010, 977-990. Elsevier, Oxford. D. Beauchemin, D.E. Matthews (Eds.).
49. Piper T., Geyer H., Gougoulidis V., Flenker U., Schänzer W. Determination of 13C/12C ratios of urinary excreted boldenone and its main metabolite 5β-androst-1-en-17β-ol-3-one. Drug Test. Anal. 2010, 2:217-224.
50. Ayotte C. Significance of 19-norandrosterone in athletes' urine samples. Br. J. Sports Med. 2006, 40:i25-i29.
51. Yamashita M., Fenn J.B. Electrospray ion source. Another variation on the free-jet theme. J. Phys. Chem. 1984, 88:4451-4459.
52. Ventura R., Fraisse D., Becchi M., Paisse O., Segura J. Approach to the analysis of diuretics and masking agents by high-performance liquid chromatography-mass spectrometry in doping control. J. Chromatogr. B: Biomed. Sci. Appl. 1991, 562:723-736.
53. Gray N., Musenga A., Cowan D.A., Plumb R., Smith N.W. A simple high pH liquid chromatography-tandem mass spectrometry method for basic compounds: application to ephedrines in doping control analysis. J. Chromatogr. A 2011, 1218:2098-2105.
54. Leinonen A., Kuuranne T., Kostiainen R. Liquid chromatography/mass spectrometry in anabolic steroid analysis - optimization and comparison of three ionization techniques: electrospray ionization, atmospheric pressure chemical ionization and atmospheric pressure photoionization. J. Mass Spectrom. 2002, 37:693-698.
55. Lips A.G.A.M., Lameijer W., Fokkens R.H., Nibbering N.M.M. Methodology for the development of a drug library based upon collision-induced fragmentation for the identification of toxicologically relevant drugs in plasma samples. J. Chromatogr. B: Biomed. Sci. Appl. 2001, 759:191-207.
56. Marquet P., Saint-Marcoux F., Gamble T.N., Leblanc J.C.Y. Comparison of a preliminary procedure for the general unknown screening of drugs and toxic compounds using a quadrupole-linear ion-trap mass spectrometer with a liquid chromatography-mass spectrometry reference technique. J. Chromatogr. B 2003, 789:9-18.
57. Maurer H.H. Position of chromatographic techniques in screening for detection of drugs or poisons in clinical and forensic toxicology and/or doping control. Clin. Chem. Lab. Med. 2004, 42:1310.
58. Dresen S., Gergov M., Politi L., Halter C., Weinmann W. ESI-MS/MS library of 1,253 compounds for application in forensic and clinical toxicology. Anal. Bioanal. Chem. 2009, 395:2521-2526.
59. Peters F.T. Recent advances of liquid chromatography-(tandem) mass spectrometry in clinical and forensic toxicology. Clin. Biochem. 2011, 44:54-65.
60. Thevis M., Kuuranne T., Geyer H., Schänzer W. Annual banned-substance review: analytical approaches in human sports drug testing. Drug Test. Anal. 2011, 3:1-14.
61. Kolmonen M., Leinonen A., Kuuranne T., Pelander A., Ojanperä I. Hydrophilic interaction liquid chromatography and accurate mass measurement for quantification and confirmation of morphine, codeine and their glucuronide conjugates in human urine. J. Chromatogr. B 2010, 878:2959-2966.
62. Maurer H.H. Liquid chromatography-mass spectrometry in forensic and clinical toxicology. J. Chromatogr. B: Biomed. Sci. Appl. 1998, 713:3.
63. Nguyen D.T.T., Guillarme D., Rudaz S., Veuthey J.-L. Fast analysis in liquid chromatography using small particle size and high pressure. J. Sep. Sci. 2006, 29:1836-1848.
64. Carr P.W., Wang X., Stoll D.R. Effect of pressure, particle size, and time on optimizing performance in liquid chromatography. Anal. Chem. 2009, 81:5342-5353.
65. Guillarme D., Ruta J., Rudaz S., Veuthey J.-L. New trends in fast and high-resolution liquid chromatography: a critical comparison of existing approaches. Anal. Bioanal. Chem. 2010, 397:1069-1082.
66. Guillarme D., Nguyen D.T.T., Rudaz S., Veuthey J.-L. Recent developments in liquid chromatography-impact on qualitative and quantitative performance. J. Chromatogr. A 2007, 1149:20-29.
67. Mazzarino M., de la Torre X., Botrè F., Gray N., Cowan D. A rapid screening LC-MS/MS method based on conventional HPLC pumps for the analysis of low molecular weight xenobiotics: application to doping control analysis. Drug Test. Anal. 2010, 2:311-322.
68. Chauve B., Guillarme D., Cléon P., Veuthey J.-L. Evaluation of various HILIC materials for the fast separation of polar compounds. J. Sep. Sci. 2010, 33:752-764.
69. Mazzarino M., Botrè F. A fast liquid chromatographic/mass spectrometric screening method for the simultaneous detection of synthetic glucocorticoids, some stimulants, anti-oestrogen drugs and synthetic anabolic steroids. Rapid Commun. Mass Spectrom. 2006, 20:3465-3476.
70. Touber M.E., van Engelen M.C., Georgakopoulus C., van Rhijn J.A., Nielen M.W.F. Multi-detection of corticosteroids in sports doping and veterinary control using high-resolution liquid chromatography/time-of-flight mass spectrometry. Anal. Chim. Acta 2007, 586:137-146.
71. Kolmonen M., Leinonen A., Kuuranne T., Pelander A., Ojanperä I. Generic sample preparation and dual polarity liquid chromatography-time-of-flight mass spectrometry for high-throughput screening in doping analysis. Drug Test. Anal. 2009, 1:250-266.
72. Guan F., Uboh C.E., Soma L.R., You Y., Liu Y., Li X. High-throughput UHPLC-MS/MS method for the detection, quantification and identification of fifty-five anabolic and androgenic steroids in equine plasma. J. Mass Spectrom. 2010, 45:1270-1279.
73. Murray G.J., Danaceau J.P. Simultaneous extraction and screening of diuretics, beta-blockers, selected stimulants and steroids in human urine by HPLC-MS/MS and UPLC-MS/MS. J. Chromatogr. B 2009, 877:3857-3864.
74. Peters R.J.B., Oosterink J.E., Stolker A.A.M., Georgakopoulos C., Nielen M.W.F. Generic sample preparation combined with high-resolution liquid chromatography-time-of-flight mass spectrometry for unification of urine screening in doping-control laboratories. Anal. Bioanal. Chem. 2010, 396:2583-2598.
75. Badoud F., Grata E., Perrenoud L., Saugy M., Rudaz S., Veuthey J.L. Fast analysis of doping agents in urine by ultra-high pressure liquid chromatography-quadrupole time-of-flight mass spectrometry. II: confirmatory analysis. J. Chromatogr. A 2010, 1217:4109-4119.
76. Badoud F., Grata E., Boccard J., Guillarme D., Veuthey J.-L., Rudaz S., Saugy M. Quantification of glucuronidated and sulfated steroids in human urine by ultra-high pressure liquid chromatography-quadrupole time-of-flight mass spectrometry. Anal. Bioanal. Chem. 2011, 1-14.
77. Strahm E., Baume N., Mangin P., Saugy M., Ayotte C., Saudan C. Profiling of 19-norandrosterone sulfate and glucuronide in human urine: implications in athlete's drug testing. Steroids 2009, 74:359-364.
78. Jian W., Edom R.W., Xu Y., Weng N. Recent advances in application of hydrophilic interaction chromatography for quantitative bioanalysis. J. Sep. Sci. 2010, 33:681-697.
79. Vonaparti A., Lyris E., Panderi I., Koupparis M., Georgakopoulos C. Direct injection horse-urine analysis for the quantification and confirmation of threshold substances for doping control. IV. Determination of 3-methoxytyramine by hydrophilic interaction liquid chromatography/quadrupole time-of-flight mass spectrometry. Drug Test. Anal. 2009, 1:365-371.
80. Marclay F., Saugy M. Determination of nicotine and nicotine metabolites in urine by hydrophilic interaction chromatography-tandem mass spectrometry: potential use of smokeless tobacco products by ice hockey players. J. Chromatogr. A 2010, 1217:7528-7538.
81. Bonfiglio R., King R.C., Olah T.V., Merkle K. The effects of sample preparation methods on the variability of the electrospray ionization response for model drug compounds. Rapid Commun. Mass Spectrom. 1999, 13:1175-1185.
82. Matuszewski B.K., Constanzer M.L., Chavez-Eng C.M. Strategies for the Assessment of matrix effect in quantitative bioanalytical methods based on HPLC-MS/MS. Anal. Chem. 2003, 75:3019-3030.
83. Food and Drug Administration Guidance for Industry: Bioanalytical Method Validation 2001, US Department of Health and Human Services, FDA, Centre for Drug Evaluation and Research, Rockville, MD.
84. Marchi I., Viette V., Badoud F., Fathi M., Saugy M., Rudaz S., Veuthey J.-L. Characterization and classification of matrix effects in biological samples analyses. J. Chromatogr. A 2010, 1217:4071-4078.
85. Van Eeckhaut A., Lanckmans K., Sarre S., Smolders I., Michotte Y. Validation of bioanalytical LC-MS/MS assays: evaluation of matrix effects. J. Chromatogr. B 2009, 877:2198-2207.
86. Dass C. Fundamentals of Contemporary Mass Spectrometry 2007, Wiley-Interscience ed., John Wiley & Sons, Hoboken.
87. Mazzarino M., Turi S., Botrè F. A screening method for the detection of synthetic glucocorticosteroids in human urine by liquid chromatography-mass spectrometry based on class-characteristic fragmentation pathways. Anal. Bioanal. Chem. 2008, 390:1389-1402.
88. Pozo O.J., Van Thuyne W., Deventer K., Van Eenoo P., Delbeke F.T. Elucidation of urinary metabolites of fluoxymesterone by liquid chromatography-tandem mass spectrometry and gas chromatography-mass spectrometry. J. Mass Spectrom. 2008, 43:394-408.
89. Vonaparti A., Lyris E., Angelis Y.S., Panderi I., Koupparis M., Tsantili-Kakoulidou A., Peters R.J.B., Nielen M.W.F., Georgakopoulos C. Preventive doping control screening analysis of prohibited substances in human urine using rapid-resolution liquid chromatography/high-resolution time-of-flight mass spectrometry. Rapid Commun. Mass Spectrom. 2010, 24:1595-1609.
90. Virus E.D., Sobolevsky T.G., Rodchenkov G.M. Introduction of HPLC/orbitrap mass spectrometry as screening method for doping control. J. Mass Spectrom. 2008, 43:949-957.
91. Thevis M., Thomas A., Schänzer W. Current role of LC-MS(/MS) in doping control. Anal. Bioanal. Chem. 2011, 1-16.
92. Broecker S., Herre S., Wüst B., Zweigenbaum J., Pragst F. Development and practical application of a library of CID accurate mass spectra of more than 2,500 toxic compounds for systematic toxicological analysis by LC-QTOF-MS with data-dependent acquisition. Anal. Bioanal. Chem. 2010, 1-17.
93. Brennen R.A., Yin H., Killeen K.P. Microfluidic gradient formation for nanoflow chip LC. Anal. Chem. 2007, 79:9302-9309.
94. Thomas A., Geyer H., Kamber M., Schänzer W., Thevis M. Mass spectrometric determination of gonadotrophin-releasing hormone (GnRH) in human urine for doping control purposes by means of LC-ESI-MS/MS. J. Mass Spectrom. 2008, 43:908-915.
95. Staub A., Rudaz S., Saugy M., Veuthey J.-L., Schappler J. Analysis of hemoglobin-based oxygen carriers by CE-UV/Vis and CE-ESI-TOF/MS. Electrophoresis 2010, 31:1241-1247.
96. Thomas A., Kohler M., Schänzer W., Kamber M., Delahaut P., Thevis M. Determination of Synacthen in urine for sports drug testing by means of nano-ultra-performance liquid chromatography/tandem mass spectrometry. Rapid Commun. Mass Spectrom. 2009, 23:2669-2674.
97. Thevis M., Thomas A., Schänzer W. Insulin. Doping in Sports: Biochemical Principles, Effects and Analysis 2010, 209-226. Springer-Verlag, Heidelberg. D. Thieme, P. Hemmersbach (Eds.).
98. Staub A., Guillarme D., Schappler J., Veuthey J.-L., Rudaz S. Intact protein analysis in the biopharmaceutical field. J. Pharm. Biomed. Anal. 2011, 55:810-822.
99. Kohler M., Walpurgis K., Thomas A., de Maree M., Mester J., Schänzer W., Thevis M. Effects of endurance exercise on the urinary proteome analyzed by 2-D PAGE and Orbitrap MS. Proteomics Clin. Appl. 2010, 4:568-576.
100. Fiehn O. Metabolomics - the link between genotypes and phenotypes. Plant Mol. Biol. 2002, 48:155-171.
101. Saugy M., Robinson N., Saudan C. The fight against doping: back on track with blood. Drug Test. Anal. 2009, 1:474-478.
102. Brown M., Dunn W.B., Ellis D.I., Goodacre R., Handl J., Knowles J.D., O'Hagan S., Spasić I., Kell D.B. A metabolome pipeline: from concept to data to knowledge. Metabolomics 2005, 1:39-51.
103. Pinel G., Weigel S., Antignac J.P., Mooney M.H., Elliott C., Nielen M.W.F., Le Bizec B. Targeted and untargeted profiling of biological fluids to screen for anabolic practices in cattle. Trends Anal. Chem. 2010, 29:1269-1280.
104. Kiss A., Jacquet A.-L., Paisse O., Flament-Waton M.-M., de Ceaurriz J., Bordes C., Gauvrit J.-Y., Lantéri P., Cren-Olivé C. Urinary signature of anabolic steroids and glucocorticoids in humans by LC-MS. Talanta 2011, 83:1769-1773.
105. Lay J.J.O., Liyanage R., Borgmann S., Wilkins C.L. Problems with the " omics" Trends Anal. Chem. 2006, 25:1046-1056.
106. Gerace E., Salomone A., Fasano F., Costa R., Boschi D., Di Stilo A., Vincenti M. Validation of a GC/MS method for the detection of two quinolinone-derived selective androgen receptor modulators in doping control analysis. Anal. Bioanal. Chem. 2011, 400:137-144.
107. Goebel C., Howe C.J., Ho K.K., Nelson A., Kazlauskas R., Trout G.J. Screening for testosterone abuse in male athletes using the measurement of urinary LH, a revision of the paradigm. Drug Test. Anal. 2009, 1:511-517.
108. Thomas A., Kohler M., Schanzer W., Delahaut P., Thevis M. Determination of IGF-1 and IGF-2, their degradation products and synthetic analogues in urine by LC-MS/MS. Analyst 2011, 136:1003-1012.
109. Holt R. Detecting growth hormone abuse in athletes. Anal. Bioanal. Chem. 2011, 1-14.
110. Galesio M., Rial-Otero R., Capelo-Martínez J.L. Comparative study of matrices for their use in the rapid screening of anabolic steroids by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. Rapid Commun. Mass Spectrom. 2009, 23:1783-1791.
111. Arsene C.G., Henrion A., Diekmann N., Manolopoulou J., Bidlingmaier M. Quantification of growth hormone in serum by isotope dilution mass spectrometry. Anal. Biochem. 2010, 401:228-235.
112. Stanley S.M.R., Wilhelmi B.S., Rodgers J.P., Bertschinger H. Immunoaffinity chromatography combined with gas chromatography - negative ion chemical ionisation mass spectrometry for the confirmation of flumethasone abuse in the equine. J. Chromatogr. B: Biomed. Sci. Appl. 1993, 614:77-86.
113. Caballero G.M., D'Angelo C.P., Fraguío M.S., Centurión O.T. Identification of 11-nor-D9-tetrahydrocannabinol-9-carboxylic acid in urine by ion trap GC-MS/MS in the context of doping analysis. J. Chromatogr. Sci. 2004, 42:540-544.
114. Chen H.-X., Deng Q.-P., Zhang L.-W., Zhang X.-X. Quantification of testosterone and epitestosterone in biological samples by capillary electrophoresis with immunoaffinity extraction. Talanta 2009, 78:464-470.
115. Dehnes Y., Lamon S., Lönnberg M. Erythropoietin (EPO) immunoaffinity columns: a powerful tool for purifying EPO and its recombinant analogues. J. Pharm. Biomed. Anal. 2010, 53:1028-1032.
116. Sanz-Nebot V., Benavente F., Vallverdú A., Guzman N.A., Barbosa J. Separation of recombinant human erythropoietin glycoforms by capillary electrophoresis using volatile electrolytes. Assessment of mass spectrometry for the characterization of erythropoietin glycoforms. Anal. Chem. 2003, 75:5220-5229.
117. Sanz-Nebot V., Benavente F., Giménez E., Barbosa J. Capillary electrophoresis and matrix-assisted laser desorption/ionization-time of flight-mass spectrometry for analysis of the novel erythropoiesis-stimulating protein (NESP). Electrophoresis 2005, 26:1451-1456.
118. Gallego R.G., Segura J. Rapid screening of plasma volume expanders in urine using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. Rapid Commun. Mass Spectrom. 2004, 18:1324-1330.
119. Giraud S., Robinson N., Mangin P., Saugy M. Scientific and forensic standards for homologous blood transfusion anti-doping analyses. Forensic Sci. Int. 2008, 179:23-33.