Erythropoietin (EPO) immunoaffinity columns-A powerful tool for purifying EPO and its recombinant analogues

Journal of Pharmaceutical and Biomedical Analysis

Volumn 53, Issue 4, 2010, Pages 1028-1032

  Dehnes, Yvette ^a   Lamon, Séverine ^b   Lönnberg, Maria ^c  

^a OSLO UNIVERSITY HOSPITAL   (Norway)

^b UNIVERSITY CENTER OF LEGAL MEDICINE   (Switzerland)

^c UPPSALA UNIVERSITY   (Sweden)

Author keywords

Affinity purification; Doping control; Erythropoietin; Isoelectric focusing; Validation

Indexed keywords

ERYTHROPOIETIN; ERYTHROPOIETIN ANTIBODY; RECOMBINANT ERYTHROPOIETIN;

ARTICLE; DOPING; DRUG PURIFICATION; IMMUNOAFFINITY CHROMATOGRAPHY; IMMUNOAFFINITY COLUMN; ISOELECTRIC FOCUSING; PRIORITY JOURNAL; PURIFICATION; RELIABILITY; REPRODUCIBILITY; SENSITIVITY AND SPECIFICITY; SIGNAL NOISE RATIO; ULTRAFILTRATION; URINALYSIS; VALIDATION PROCESS;

CHROMATOGRAPHY, AFFINITY; DOPING IN SPORTS; ERYTHROPOIETIN; ERYTHROPOIETIN, RECOMBINANT; HUMANS; ISOELECTRIC FOCUSING; REPRODUCIBILITY OF RESULTS;

EID: 77955562805     PISSN: 07317085     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jpba.2010.06.017     Document Type: Article

References (19)

1. Lasne F., de Ceaurriz J. Recombinant erythropoietin in urine. Nature 2000, 405:635.
2. Lasne F., Martin L., Crepin N., de Ceaurriz J. Detection of isoelectric profiles of erythropoietin in urine. Anal. Biochem. 2002, 311:119-126.
3. Lasne F. Double-blotting: a solution to the problem of nonspecific binding of secondary antibodies in immunoblotting procedures. J. Immunol. Methods 2003, 276:223-226.
4. Peltre G., Thormann W. Evaluation Report of the Urine EPO Test 2003, Council of the World Anti-Doping Agency (WADA), Paris and Bern.
5. Lasne F., Martin L., Martin J.A., de Ceaurriz J. Isoelectric profiles of human erythropoietin are different in serum and urine. Int. J. Biol. Macromol. 2007, 41:354-357.
6. Mallorqui J., Gutierrez-Gallego R., Segura J., de Bolos C., Pascual J.A. New screening protocol for recombinant human erythropoietins based on differential elution after immunoaffinity purification J. Pharm. Biomed. Anal. 2010, 51:255-259.
7. Hennion M.C., Pichon V. Immuno-based sample preparation for trace analysis. J. Chromatogr. A 2003, 1000:29-52.
8. Franke W.W., Heid H. Pitfalls, errors and risks of false-positive results in urinary EPO drug tests. Clin. Chim. Acta 2006, 373:189-190.
9. Kahn A., Baker M. Reply by the authors to "New urinary EPO drug testing method using two-dimensional gel electrophoresis" Clin. Chim. Acta 2006, 379:173-175.
10. Beullens M., Delanghe J.R., Bollen M. False-positive detection of recombinant human erythropoietin in urine following strenuous exercise. Blood 2006, 107:4711-4713.
11. Reichel C. Identification of zinc-alpha-2-glycoprotein binding to clone AE7A5 antihuman EPO antibody by means of nano-HPLC and high-resolution high-mass accuracy ESI-MS/MS. J. Mass Spectrom. 2008, 43:916-923.
12. Skibeli V., Nissen-Lie G., Torjesen P. Sugar profiling proves that human serum erythropoietin differs from recombinant erythropoietin. Blood 2001, 98:3626-3634.
13. Desharnais P., Ayotte C. Towards the screening of urinary EPO by SDS-PAGE analysis. Recent Advances in Doping Analysis 2007, vol. 15. Sportverlag Strauβ, Cologne, 303 p.
14. Kohler M., Ayotte C., Desharnais P., Flenker U., Lüdke S., Thevis M., Völker-Schänzer E., Schänzer W. Discrimination of urinary recombinant and enogenous erythropoietin by calculating relative mobility values from SDS gels. Int. J. Sports Med. 2008, 29:1-6.
15. Reichel C., Kulovics R., Jordan V., Watzinger M., Geisendorfer T. SDS-PAGE of recombinant and endogenous erythropoietins: benefits and limitations of the method for application in doping control. Drug Test. Anal. 2009, 1:43-50.
16. Bajla I., Holländer I., Minichmayr M., Gmeiner G., Reichel C. GASepo-a software solution for quantitative analysis of digital images in Epo doping control. Comput. Methods Prog. Biomed. 2005, 80:246-270.
17. C. Ayotte, J.A. Pascual, G. Gmeiner, C. Reichel, F. Lasne, M. Saugy, WADA Technical Document TD2009EPO.
18. Lamon S., Robinson N., Mangin P., Saugy M. Detection window of Darbepoietin-α following one singe subcutaneous injection. Clin. Chim. Acta 2007, 379:145-149.
19. ISO/IEC 17025, accessed on 10 June 2010. http://www.isoiec17025.com/.